Microscopy preprints – new tools and techniques in imaging

Here is a curated selection of preprints posted recently on new tools and techniques in imaging. Let us know if we are missing any recent preprints that are on your reading list!

Reusable Microfluidic Chambers for Single-Molecule Microscopy
Janan Alfehaid, Sineth G. Kodikara, Tuqa Alhajri, Mohammad Lutful Kabir, Hamza Balci

Model-free machine learning-based 3D single molecule localisation microscopy
Miguel A. Boland, Jonathan P. E. Lightley, Edwin Garcia, Sunil Kumar, Chris Dunsby, Seth Flaxman, Mark A. A. Neil, Paul M. W. French, Edward A. K. Cohen

Ultrasensitive in vivo infrared spectroscopic imaging via oblique photothermal microscopy
Mingsheng Li, Sheng Xiao, Hongli Ni, Guangrui Ding, Yuhao Yuan, Carolyn Marar, Jerome Mertz, Ji-Xin Cheng

FASER: A TOOL TO SIMULATE PSF DISTORTIONS IN STED MICROSCOPY
Stephane Bancelin, Johannes Roos, U. Valentin Nägerl

HiExM: high-throughput expansion microscopy enables scalable super-resolution imaging
John H. Day, Catherine Marin Della Santina, Pema Maretich, Alexander L. Auld, Kirsten K. Schnieder, Tay Shin, Edward S. Boyden, Laurie A. Boyer

Directed evolution of the multicopper oxidase laccase for cell surface proximity labeling and electron microscopy
Song-Yi Lee, Heegwang Roh, David Gonzalez-Perez, Mason R Mackey, Keun-Young Kim, Daniel Hoces, Colleen McLaughlin, Stephen R Adams, Khanh Nguyen, David J Luginbuhl, Liqun Luo, Namrata Udeshi, Steven A Carr, Rogelio A Hernandez-Lopez, Mark Ellisman, Miguel Alcalde, Alice Y Ting

Adaptive optical correction in in vivo two-photon fluorescence microscopy with neural fields
Iksung Kang, Hyeonggeon Kim, Ryan Natan, Qinrong Zhang, Stella X. Yu, Na Ji

CelFDrive: Artificial Intelligence assisted microscopy for automated detection of rare events
Scott Brooks, Sara Toral-Pérez, David S. Corcoran, Karl Kilborn, Brian Bodensteiner, Hella Baumann, Nigel J. Burroughs, Andrew D. McAinsh, Till Bretschneider

Kilohertz volumetric imaging of in-vivo dynamics using squeezed light field microscopy
Zhaoqiang Wang, Ruixuan Zhao, Daniel A Wagenaar, Diego Espino, Liron Sheintuch, Ohr Benshlomo, Wenjun Kang, Calvin K. Lee, William C. Schmidt, Aryan Pammar, Enbo Zhu, Jing Wang, Gerard C.L. Wong, Rongguang Liang, Peyman Golshani, Tzung K. Hsiai, Liang Gao

A Versatile Light Field Microscopy Platform for Multi-purpose Dynamic Volumetric Bio-imaging
Yangyang Bai, Matt Jones, Lauro Sebastian Ojeda, Janielle Cuala, Lynne Cherchia, Kate L. White, Senta K. Georgia, Scott E. Fraser, Thai V. Truong

The Roboscope: Smart and Fast Microscopy for Generic Event-Driven Acquisition
Julia Bonnet, Youssef El-Habouz, Célia Martin, Maelle Guillout, Louis Ruel, Baptiste Giroux, Claire Demeautis, Benjamin Mercat, Otmane Bouchareb, Jacques Pécreaux, Marc Tramier

Carbon nanodots as a red emissive fluorescent probe for the super-resolution microscopy of DNA dynamics during paclitaxel treatment
Richa Garg, Kush Kaushik, Abdul Salam, Runmi Kundu, Shilpa Chandra, Chayan Kanti Nandi

Live-HPF CLEM using the HPM Live µ: Finding back all needles in every haystack. The last frontier in CLEM
Xavier Heiligenstein, Chie Kodera, Yann Bret, Frederic Eyraud, Vincent Muczynski, Jérôme Heiligenstein, Martin Belle

High-speed 3D Imaging with 25-Camera Multifocus Microscope
Eduardo Hirata Miyasaki, Antone A. Bajor, Gustav M. Pettersson, Maximilian L. Senftleben, Kaitlyn E. Fouke, Thomas G.W. Graham, Demis D. John, Jennifer R. Morgan, Gal Haspel, Sara Abrahamsson

Achieving Single Cell Acoustic Localisation with Deactivation Super Resolution
Cameron A. B. Smith, Mengtong Duan, Jipeng Yan, Laura Taylor, Mikhail G. Shapiro, Meng-Xing Tang

The eduWOSM: a benchtop advanced microscope for education and research
N.J. Carter, D.S. Martin, J.E. Molloy, R.A. Cross

Biological volume EM with focused Ga ion beam depends on formation of radiation-resistant Ga-rich layer at block face
Zijie Yang, Joshua Kim, Guofeng Zhang, Maria A. Aronova, Richard D. Leapman

Ten-Fold Expansion MALDI Mass Spectrometry Imaging of Tissues and Cells at 500 nm Resolution
Chengyi Xie, Jianing Wang, Xin Diao, Lei Guo, Thomas Ka-Yam Lam, Ruxin Li, Yanyan Chen, Yue Zhang, Xiaoxiao Wang, Jiacheng Fang, Zongwei Cai

TWINKLE: An open-source two-photon microscope for teaching and research
Manuel Schottdorf, P. Dylan Rich, E. Mika Diamanti, Albert Lin, Sina Tafazoli, Edward H. Nieh, Stephan Y. Thiberge

A one-step protocol to generate impermeable fluorescent HaloTag substrates for in situ live cell application and super-resolution imaging
Kilian Roßmann, Siqi Sun, Christina Holmboe Olesen, Maria Kowald, Eleni Tapp, Ulrich Pabst, Marie Bieck, Ramona Birke, Brenda C. Shields, PyeongHwa Jeong, Jiyong Hong, Michael R. Tadross, Joshua Levitz, Martin Lehmann, Noa Lipstein, Johannes Broichhagen

Tissue stiffness mapping by light sheet elastography
Min Zhu, Kaiwen Zhang, Evan C. Thomas, Ran Xu, Brian Ciruna, Sevan Hopyan, Yu Sun

Fluorescent anionic cyanine plasma membrane probes for live cell and in vivo imaging
Dmytro I. Danylchuk, Igor Khalin, Yelisetty V. Suseela, Severin Filser, Nikolaus Plesnila, Andrey S. Klymchenko

ExoSloNano: Multi-Modal Nanogold Tags for identification of Macromolecules in Live Cells & Cryo-Electron Tomograms
Lindsey N. Young, Alice Sherrard, Huabin Zhou, Farhaz Shaikh, Joshua Hutchings, Margot Riggi, Michael K. Rosen, Antonio J. Giraldez, Elizabeth Villa

Innovative Strategies for Enhancing Signal-to-Noise Ratio in Single-molecule Imaging: The Influence of Molecular Motion
Gayun Bu, Gyeongho Kang, Seongyu Park, Jae-Hyung Jeon, Jong-Bong Lee

MINFLUX — molecular resolution with minimal photons
Lukas Scheiderer, Zach Marin, Jonas Ries

Decoding mEos4b Day-Long Maturation and Engineering Fast Maturing Variants
Arijit Maity, Oleksandr Glushonkov, Isabel Ayala, Pascale Tacnet, Jip Wulffelé, Philippe Frachet, Bernhard Brutscher, Dominique Bourgeois, Virgile Adam

Vortex Interference Enables optimal 3D Interferometric Nanoscopy
Wei Wang, Zengxin Huang, Yilin Wang, Hangfeng Li, Pakorn Kanchanawong

Cytosolic Enhanced Dark Epac-Based FRET Sensors for Intracellular cAMP detection in live cells via FLIM
Giulia Zanetti, Jeffrey B. Klarenbeek, Kees Jalink

Optimising image capture for low-light widefield quantitative fluorescence microscopy
Zane Peterkovic, Avinash Upadhya, Christopher Perrella, Admir Bajraktarevic, Ramses Bautista Gonzalez, Megan Lim, Kylie R Dunning, Kishan Dholakia

Surface-Emitting Resonator Interference Microscopy for Label-Free Monitoring of Membrane Dynamics
Chaoyang Gong, Yu-Cheng Chen

Crossed laser phase plates for transmission electron microscopy
Petar N. Petrov, Jessie T. Zhang, Jeremy J. Axelrod, Holger Müller

Increasing the spatial bandwidth product in Light Field Microscopy with remote scanning
Aymerick Bazin, Amaury Badon

Semicircular illumination scanning transmission electron microscopy
Akira Yasuhara, Fumio Hosokawa, Sadayuki Asaoka, Teppei Akiyama, Tomokazu Iyoda, Chikako Nakayama, Takumi Sannomiya

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