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This 3D image highlights the star-shaped basal cell network encircling milk-producing alveolar units in the murine mammary gland. The contractile nature of these cells enables milk ejection into the mouths of feeding pups. The tissue was cleared via CUBIC, immunostained for smooth muscle actin (SMA) and imaged using the Leica SP8 inverted laser scanning microscope. The z-stacks, covering a distance of 24um, were combined into this final, maximum projection image in ImageJ. Image by Krystyna Gieniec.

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Recent posts

Microscopy preprints – applications in cell biology

Posted by , on 1 July 2022

Here is a curated selection of preprints published recently. In this post, we focus specifically on preprints using microscopy tools in cell biology.

FocalPlane News Digest

Posted by , on 29 June 2022

Talks, training and workshops Don’t miss our FocalPlane features… webinar on quantitative phase imaging and its cell biological applications. If you haven’t already, book your place https://focalplane.biologists.com/focalplane-features/ Have you submitted an abstract for the Abercrombie Meeting yet? Fear not – the submission deadline has been extended: https://www.rms.org.uk/rms-event-calendar/2022-events/abercrombie-meeting-2022/abstracts.html The Microscopy and Microanalysis (M&M) Meeting is taking

Using an autocollimator to align 4f systems

Posted by , on 29 June 2022

Graeme Johnstone, Brian Patton, Department of Physics and SUPA, University of Strathclyde, Glasgow G4 0NG, United Kingdom Modern microscope systems comprise many optical elements with precise alignment tolerances. As such, as the complexity of the system increases, the challenge to align them rapidly becomes a major hurdle to implementing home-built designs. One recurring problem is

Imaging Specialist – University of Warwick

Posted by , on 28 June 2022

Permanent Position, Full time – 36.5 hours per week The postholder will provide expert-level hands-on technical and user support for optical microscopy. The appointee will need to combine enthusiasm and careful attention to detail with adaptability. The post would suit an individual with a background in physics who wants to apply their skills to advance

An interview with Flavio Zolessi

Posted by , on 28 June 2022

Dr. Flavio Zolessi is a group leader and head of the Cell Biology Unit at the Faculty of Science at Universidad de la República in Montevideo, Uruguay. He is also an Associate Researcher at Institut Pasteur Montevideo, Researcher Grade 4 of Programa de Desarrollo de las Ciencias Básicas (PEDECIBA) and Reseacrher Level II at the

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Updated on 28 June 2022