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This image by Jamie Whitelaw was the Judges Choice winner of the image competition at the Scottish Microscopy Society Symposium. The image shows NckAP1 KO B16-F1 cells migrating through matrigel in a circular invasion assay. Cells were fixed and stained using Phalloidin in magenta, Tubulin in cyan and DAPI in yellow. Z-stacked, tile scan images were taken on the Zeiss 880 AiryScan at 20X objective and displayed at a max projection. Note that for some unknown reason, the tubulin staining appears reduced in follower cells compared to the leader cells, while F-actin remains uniform throughout.

Jamie obtained a PhD in Molecular Parasitology at the University of Glasgow, after which, he moved to the CRUK- Beatson Institute for cancer research as a Post-Doctoral researcher with Prof Laura Machesky.  He is interested in cancer cell migration and invasion and with that he enjoys spending time at the microscope, primarily using various confocal and super-resolution systems.  His research has focused on the interplay between the cell’s cytoskeleton and mechanosensing.  More recently he has also taken on a project using FLIM-FRET to study signaling mechanisms across collective cell migration.

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Updated on 20 December 2021