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The FocalPlane image competition 2020 winner is Tejeshwar Rao from Alexa L. Mattheyses’ lab at the University of Alabama for his image ‘Owl Eyes’ (above). The image depicts duplicated Cos-7 cell plated on a tension gauge tether surface. After stimulation with epidermal growth factor (EGF), the cell was fixed and stained for F-actin (orange hot) using phalloidin and for focal adhesions (blue-green) using a paxillin antibody. Integrin forces, i.e. open tension probes, are shown in greyscale. Total Internal Reflection Fluorescence (TIRF) microscopy was employed to acquire the images and processed using NIS elements and reconstructed in Fiji (ImageJ). More about Tejeshwar’s work coming on FocalPlane soon.

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Microscopy Open Access collection 2020

Posted by , on 19 October 2020

Here on FocalPlane, Open Access Week is especially close to our hearts as it coincides with the birthday of the father of microscopy, Antonie van Leeuwenhoek (24 October). So every year for Open Access Week, we will be listing all the microscopy-related Open Access articles from The Company of Biologists journals on FocalPlane under the

Technology highlights – Spinning Disk Microscopy

Posted by , on 14 October 2020

Interview with Stoyno Stoynov, Ph.D. from the Center of Advanced Microscopy, Bulgarian Academy of Sciences, Bulgaria. Tell us a bit about the facility you run and what your focus is. The Bulgarian Node of Euro-Bioimaging ERIC is based in the Institute of Molecular Biology of the Bulgarian Academy of Sciences, Sofia. Our node is part

How I got into the Flamingo project, a new way of sharing custom-built light sheet microscopes

Posted by , on 12 October 2020

Sharing microscopy technology is crucial for biomedical research. This is the story how I joined Jan Huisken and his team to give other scientists quick access to custom-built light sheet microscopes.

LSFM series – Surfing on the data freak wave! Part II: Before imaging: Know your sample (geometry)

Posted by , on 10 October 2020

This post, part of the blog series “LSFM series – Surfing on the data freak wave!”, discusses (a) Sample preparation, (b) Light interaction with matter, (c) sample alignment and (d) checking fluorescence and calibration

FocalPlane image competition voting is open!

Posted by , on 5 October 2020

After 3 weeks of submissions we are happy to announce that the submissions gallery is now up and running for 1 week (until 12 October, 5pm UK time)! We have 83 fantastic entries from 45 different people who have used a range of different microscopy techniques to acquire their beautiful images. To see our competition

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Updated on 15 October 2020