FocalPlane - Where biology meets microscopy

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The FocalPlane image competition 2020 winning image ‘Owl Eyes’ has made the Journal of Cell Science issue 21 cover! Tejeshwar Rao from Alexa L. Mattheyses’ lab at the University of Alabama submitted this image depicting duplicated Cos-7 cell plated on a tension gauge tether surface. After stimulation with epidermal growth factor (EGF), the cell was fixed and stained for F-actin (orange hot) using phalloidin and for focal adhesions (blue-green) using a paxillin antibody. Integrin forces, i.e. open tension probes, are shown in greyscale. Total Internal Reflection Fluorescence (TIRF) microscopy was employed to acquire the images and processed using NIS elements and reconstructed in Fiji (ImageJ).

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