This image by Jamie Whitelaw was the Judges Choice winner of the image competition at the Scottish Microscopy Society Symposium. The image shows NckAP1 KO B16-F1 cells migrating through matrigel in a circular invasion assay. Cells were fixed and stained using Phalloidin in magenta, Tubulin in cyan and DAPI in yellow. Z-stacked, tile scan images were taken on the Zeiss 880 AiryScan at 20X objective and displayed at a max projection. Note that for some unknown reason, the tubulin staining appears reduced in follower cells compared to the leader cells, while F-actin remains uniform throughout.
Jamie obtained a PhD in Molecular Parasitology at the University of Glasgow, after which, he moved to the CRUK- Beatson Institute for cancer research as a Post-Doctoral researcher with Prof Laura Machesky. He is interested in cancer cell migration and invasion and with that he enjoys spending time at the microscope, primarily using various confocal and super-resolution systems. His research has focused on the interplay between the cell’s cytoskeleton and mechanosensing. More recently he has also taken on a project using FLIM-FRET to study signaling mechanisms across collective cell migration.
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Janelia+EMBL BioImaging Seminar Series: How We Started a Successful Seminar Series during the Pandemic
Posted by Ulrike Boehm, on 25 January 2022How we started a global BioImaging seminar series in the middle of a global pandemic…
Posted by FocalPlane, on 21 January 2022Here is a selection of interesting news, publications and discussions related to microscopy that happened in the last two weeks. Thank you to our ‘FocalPlane reporter’ Martin Jones who helped us create this list. Twitter Microscopes and imaging tools: Cryo-Expansion microscopy (Cryo-ExM) published in Nature Methods https://twitter.com/MarineLaporte8/… New quantitative biosensors that work in live animals https://twitter.com/AndrewGYork/…
Posted by FocalPlane, on 14 January 2022Here is a curated selection of preprints published recently. In this post, we focus specifically on preprints using microscopy tools in different fields such as cell biology, plant biology, microbiology, and neuroscience. Nanoscale details of mitochondrial fission revealed by cryo-electron tomography. Shrawan Kumar Mageswaran, Danielle Ann Grotjahn, Xiangrui Zeng, Benjamin Asher Barad, Michaela A Medina,
Posted by FocalPlane, on 12 January 2022Join us for our first FocalPlane features… webinar of 2022 on Monday 17 January to learn more about Correlative Light Electron Microscopy (CLEM) with Yannick Schwab, Ori Avinoam, and Alexandra Pacureanu. The talks will be hosted in Remo, our browser-based conferencing platform. Following the Q&A session we will host a more informal session especially designed
Posted by FocalPlane, on 7 January 2022Here is a selection of interesting news, publications and discussions related to microscopy that happened in the last two weeks. Thank you to our ‘FocalPlane reporters’ Martin Jones and Andrey Andreev who helped us create this list. Twitter Microscopes and imaging tools: Temperature-sensitive optogenetic tools https://twitter.com/BugajLab/… Foldscope now has a TIRF adaptor https://twitter.com/PrakashLab/… Confocal vs
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Microscopy-related articles from our journals
- L-selectin regulates human neutrophil transendothelial migration. Journal of Cell Science 2021 134: jcs.250340
- The 20S proteasome activator PA28γ controls the compaction of chromatin. Journal of Cell Science 2021 134: jcs.257717
- Tissue-resident macrophages regulate lymphatic vessel growth and patterning in the developing heart. Development 2021 148: dev.194563
- Disruption of a Hedgehog-Foxf1-Rspo2 signaling axis leads to tracheomalacia and a loss of Sox9+ tracheal chondrocytes. Disease Models & Mechanisms 2021 14: dmm046573
- Mutations in the splicing regulator Prp31 lead to retinal degeneration in Drosophila. Biology Open 2021 10: bio052332
Microscopy-related preprint highlights
- InsectBrainDatabase – A unified platform to manage, share, and archive morphological and functional data.
- Endothelial junctional membrane protrusions serve as hotspots for neutrophil transmigration.
- Ciliary control of meiotic chromosomal pairing mechanics and germ cell morphogenesis. doi: 10.1242/prelights.27402
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