The FocalPlane image competition 2020 winning image ‘Owl Eyes’ has made the Journal of Cell Science issue 21 cover! Tejeshwar Rao from Alexa L. Mattheyses’ lab at the University of Alabama submitted this image depicting duplicated Cos-7 cell plated on a tension gauge tether surface. After stimulation with epidermal growth factor (EGF), the cell was fixed and stained for F-actin (orange hot) using phalloidin and for focal adhesions (blue-green) using a paxillin antibody. Integrin forces, i.e. open tension probes, are shown in greyscale. Total Internal Reflection Fluorescence (TIRF) microscopy was employed to acquire the images and processed using NIS elements and reconstructed in Fiji (ImageJ).
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Posted by Paola Moreno-Roman, on 18 November 2020Foldscope Instruments, Inc. is a company that was founded in 2016. We develop low-cost scientific tools with the goal of making science accessible to everyone around the world. In 2018, the Foldscope team visited Peru, Argentina, and Brazil. At the time, I was a Stanford graduate student in Biology and, due to school-related commitments, I
Posted by Johanna Bischof, on 11 November 2020Interview with Enzo Terreno, Ph.D. from the University of Torino, Italy. Please tell us a bit about yourself and the facility where you work. My name is Enzo Terreno, and I have the pleasure to be the Coordinator of the Multi-Modal Molecular Imaging (MMMI) Italian Node belonging to Euro-BioImaging. This is a multi-sited Node comprising
Sponsored by Andor, on 4 November 2020Multiplexing in cell biology is the unveiling of several (Xn) RNAs in its 2D or 3D biological context. Multiplexing has become a hot topic in neurosciences, oncobiology, disease target diagnostics, development, behavioural studies, etc. Several techniques have been developed to allow multiplex imaging; examples of such are FISSEQ, instaSEQ, osmFISH, STARmap, MERFISH and seqFISH. The advantage of
Posted by Johanna Bischof, on 28 October 2020Interview with Antti Isomäki, PhD from the Biomedicum Imaging Unit of the University of Helsinki, Finland and Dalibor Pánek, PhD from the BIOCEV in Prague, Czech Republic. Tell us a bit about who you are and where your facility is based. AI: My name is Antti Isomäki and I am an optical physicist by training.
Sponsored by Andor, on 27 October 2020Challenge Background Understanding the molecular basis of development, brain function, neurodegenerative diseases, cancer, and behaviour is an enormous task. Up until recently, RNAs could be sequenced in bulk, or at the single-cell level, but unfortunately, the tissue environment information was lost. With smFISH  (single molecule FISH), the spatial information is retained, but this is
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Microscopy-related articles from our journals
- Experimental toolbox for quantitative evaluation of clathrin-mediated endocytosis in the plant model Arabidopsis. Journal of Cell Science 2020 133: jcs248062
- The visible skeleton 2.0: phenotyping of cartilage and bone in fixed vertebrate embryos and foetuses based on X-ray microCT. Development 2020 147: dev187633
- A lineage-tracing tool to map the fate of hypoxic tumour cells Disease Models & Mechanisms 2020 13: dmm044768
- Functional effect of vaterite – the presence of an alternative crystalline structure in otoliths alters escape kinematics of the brown trout. Journal of Experimental Biology 2020 223: jeb222034
- Nanomorphological and mechanical reconstruction of mesenchymal stem cells during early apoptosis detected by atomic force microscopy. Biology Open 2020 9: bio048108
Microscopy-related preprint highlights
- Improving the toolkit for 3D imaging with deep learning: HyLFM and HyLFM-Net. doi: 10.1242/prelights.25619
- Configuring high-resolution light sheet imaging to use one objective brings fast volumetric imaging to any sample format. doi: 10.1242/prelights.21204
- Microscopy and zebrafish: Understanding vasculature– part III. doi: 10.1242/prelights.24427
From the gallery
- Confocal microscopyApply
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- Single plane illumin..ation microscopy (SPIM / Light sheet)Apply
- Total internal refle..ction fluorescence (TIRF) microscopyApply
- Electron microscopyApply
- Multi-photon microsc..opyApply
- Label-free microscop..yApply
- Genetic probesApply
- Optical manipulationApply
- Functional imagingApply