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Kidney elastic fiber network changes in Marfan's Syndrome

Posted by Mario Elias Ortega Sandoval, on 19 July 2024

by de Souza RB*, Meek KM#, Lewis PN#, Pereira LV*. *Department of Genetics and Evolutionary Biology, University of São Paulo, Brazil #StructuralbBiophysics Research Group, School of Optometry and Vision Sciences of the United Kingdom, Cardiff University, UK Challenge Marfan’s syndrome (MFS) is an autosomal dominant disorder that affects the connective tissue, resulting from a mutation

Featured image with Jovan Brockett

Posted by FocalPlane, on 19 July 2024

Our featured image, acquired by Jovan Brockett, shows a Drosophila melanogaster embryo undergoing syncytial nuclear divisions during early embryonic development. It was prepared using PFA fixation and hand dissected to remove the outer vitelline membrane. The image was captured using a Nikon Ti-E system fitted with a Yokagawa CSU-X1 spinning disk head, Hamamatsu Orca Flash

Microscopy preprints - applications in biology

Posted by FocalPlane, on 12 July 2024

Here is a curated selection of preprints published recently. In this post, we share preprints that use microscopy tools to answer questions in biology.

Featured image with Hoang Anh Le

Posted by FocalPlane, on 5 July 2024

Our featured image, acquired by Hoang Anh Le, shows an Ewing’s sarcoma cancer cell plated on a fibronectin-coated surface. The cell was fixed with 4% formaldehyde and stained using phalloidin (white) and DAPI (in pink). The sample was imaged using a confocal Zeiss 880 microscope with Airyscan.

Microscopy preprints - new tools and techniques in imaging

Posted by FocalPlane, on 28 June 2024

Here is a curated selection of preprints posted recently on new tools and techniques in imaging. Let us know if we are missing any recent preprints that are on your reading list!

Featured image with Theresa Wiesner

Posted by FocalPlane, on 21 June 2024

Our featured image, acquired by Theresa Wiesner, shows a neuron in dissociated hippocampal culture and was imaged using highly inclined thin illumination microscopy (HiLO). The neuron is transfected with a neurotransmitter (glutamate) sensor and shown in orange. The image was acquired using Nikon Elements software / microscope and post-processing was done using FIJI (ImageJ). Find

Microscopy Training Needs Survey

Posted by Georgina Fletcher, on 20 June 2024

The Royal Microscopical Society (RMS) are currently running a Training Needs Survey, which will be live until 30th July, 2024. Fill in the survey here: https://www.surveymonkey.com/r/66TLZQW In a recent review exercise the RMS established that in 2014 over 30 training courses for different microscopy modalities and techniques were running in the UK. By 2024 this

Microscopy preprints – bioimage analysis

Posted by FocalPlane, on 19 June 2024

Here is a curated selection of preprints published recently. In this post, we focus specifically on bioimage analysis.

And the winner is…

Posted by FocalPlane, on 7 June 2024

In an exciting turn of events, we are delighted to announce that the winner of the FocalPlane/elmi (European Light Microscopy Initiative) is the electron micrograph, ‘Glorious Lymphocyte’ from Rossana Melo! The image shows a leukocyte (lymphocyte) trapped among aggregates of red blood cells in a small lung vessel of a patient with asthma. The image

Microscopy preprints - applications in biology

Posted by FocalPlane, on 31 May 2024

Here is a curated selection of preprints published recently. In this post, we share preprints, released before 24 May, that use microscopy tools to answer questions in biology.

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Microscopy-related articles from our journals

HCR spectral imaging: 10-plex, quantitative, high-resolution RNA and protein imaging in highly autofluorescent samples Development 2024 151: dev202307
filoVision – using deep learning and tip markers to automate filopodia analysis J Cell Sci 2024 137: jcs261274
C omputational tools for quantifying actin filament numbers, lengths, and bundling Biology Open 2024 13: bio.060267

Microscopy-related preprint highlights

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