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Displaying posts in the category: Case studies

How volumeEM (vEM) can help map neuronal circuits

Posted by , on 2 March 2022

by Nadine Randel, University of Cambridge, UK DOI:10.5281/zenodo.6320414 Challenge The nervous system processes sensory information and generates different behavioural outputs accordingly. Synaptic resolution wiring diagrams form a basis for our understanding of how the brain works. Such a connectome provides the synaptic connectivity between all neurons and allows to identify all potential neuronal pathways which

Revealing the spatial regulation of the transcriptome using Split-FISH

Sponsored by Andor, on 9 February 2022

Featured Lab: The Chen Lab, Genome Institute of Singapore  As cells develop and function in tissues, many changes occur not just at the level of RNA transcription, but also at the larger level of spatial organisation of cellular gene expression within the tissue. Studying these changes to RNA profiles at the single-cell level can thus provide

Behind the Screen - behind the scenes of setting up a dynamic screening platform

Posted by , on 22 November 2021

The Cell Biophysics lab has focused on FRET (Forster Resonance Energy Transfer) as it is a powerful technique to investigate dynamic protein-protein interactions, along with studying almost every aspect of cellular signaling with biosensors. FRET is detected either by ratioing the  intensities of the FRET donor and acceptor,  or by FLIM (Fluorescence Lifetime Imaging ;

Asymmetric cell divisions in 3 dimension stem cell colonies

Posted by , on 27 September 2021

When looking at the development of a multicellular organism, for example a human, the first striking feature is the progressive increase in cell numbers due to successive divisions, from one single tiny cell 80 mm in diameter to a 3.5 kg ball of organised cells forming a new-born baby. The way cells divide, in particular

SRRF-Stream+ Super-Resolution Microscopy Accessible to All

Sponsored by Andor, on 12 August 2020

Fast, reliable & live-cell compatible Super-Resolution Science has limits imposed by the laws of physics that constrain discoveries and the advance of knowledge. In microscopy, up until the beginning of the XXI century, the diffraction limit of light was an unbreakable barrier. This law of physics imposes that two points could not be resolved (clearly

Fixation artifacts and how to minimize them

Posted by , on 7 July 2020

Sample preparation is the first step for having high-quality images that will impress everyone, but it is often overlooked. Many times I have tried to help others improve their microscope images, only to find out that improvement was not possible due to the quality of the sample. No matter how expensive your microscope is, you