Case studies - FocalPlane

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A journey of a novice in immunofluorescence assay

Posted by Seth Domfeh, on 15 November 2024

I am Seth Domfeh, a lecturer and an early-career researcher from the Department of Biochemistry and Biotechnology, Kwame Nkrumah University of Science and Technology, Ghana. I am delighted to share my experience before and during my research visit to the Africa Microscopy Initiative (AMI) Imaging Centre, Institute of Infectious Disease and Molecular Medicine, University of

Kidney elastic fiber network changes in Marfan's Syndrome

Posted by Mario Elias Ortega Sandoval, on 19 July 2024

by de Souza RB*, Meek KM#, Lewis PN#, Pereira LV*. *Department of Genetics and Evolutionary Biology, University of São Paulo, Brazil #StructuralbBiophysics Research Group, School of Optometry and Vision Sciences of the United Kingdom, Cardiff University, UK Challenge Marfan’s syndrome (MFS) is an autosomal dominant disorder that affects the connective tissue, resulting from a mutation

FocalPlane features... Samrat Mukhopadhyay with PicoQuant

Posted by FocalPlane, on 31 January 2024

In case you missed ‘FocalPlane features…’, here is the recording of the webinar given by Samrat Mukhopadhyay on 'A deep dive into biomolecular condensates using single-molecule FRET', which we co-hosted with PicoQuant.

Targeted vCLEM of heterogeneous samples

Posted by Mario Elias Ortega Sandoval, on 15 January 2024

by Karel Mocaer* and Paolo Ronchi# *Schwab Team and #EMCF, EMBL Heidelberg, Germany Challenge Studying cells or events within their biological context (i.e. in tissues, ecosystem etc…) is highly relevant. However, volume EM techniques are limited in the volume that can be acquired. Therefore, targeting approaches are needed to characterize specific regions of interest. This

Deciphering cardiac multiscale organization

Posted by Georgina Fletcher, on 11 December 2023

by Sandra Rugonyi and Claudia López Oregon Health & Science University, Portland Oregon, USA Challenge The morphology, microstructure, and ultrastructure of heart tissues is exquisitely organized to optimize cardiac efficiency. In cases of cardiac malformations, known as congenital heart disease (CHD), spatially heterogeneous and abnormal ultrastructural patterns emerge as early as fetal stages that worsen

Upcoming webinar: A Deep Dive into Biomolecular Condensates Using Single-Molecule FRET

Sponsored by PicoQuant, on 22 November 2023

In a brand-new paper, Ashish Joshi and colleagues from the team of Samrat Mukhopadhyay at IISER Mohali take a close look at the molecular events during phase separation of the FUS protein with single-molecule fluorescence methods. If you want to learn more about these techniques and what they revealed about the complex behavior of the

Have you heard of Euro-BioImaging?

Posted by FocalPlane, on 23 March 2023

Euro-BioImaging is a scheme that sounds too good to be true: access to advanced microscopy technologies and the experts that are responsible for them, what’s not to like! The 35 imaging facilities, known as Nodes, that make up the infrastructure of Euro-BioImaging are located across 16 European countries and are accessible to all scientists regardless

How volumeEM (vEM) can help map neuronal circuits

Posted by FocalPlane, on 2 March 2022

by Nadine Randel, University of Cambridge, UK DOI:10.5281/zenodo.6320414 Challenge The nervous system processes sensory information and generates different behavioural outputs accordingly. Synaptic resolution wiring diagrams form a basis for our understanding of how the brain works. Such a connectome provides the synaptic connectivity between all neurons and allows to identify all potential neuronal pathways which

Revealing the spatial regulation of the transcriptome using Split-FISH

Behind the Screen - behind the scenes of setting up a dynamic screening platform

Posted by Sravasti Mukherjee, on 22 November 2021

The Cell Biophysics lab has focused on FRET (Forster Resonance Energy Transfer) as it is a powerful technique to investigate dynamic protein-protein interactions, along with studying almost every aspect of cellular signaling with biosensors. FRET is detected either by ratioing the intensities of the FRET donor and acceptor, or by FLIM (Fluorescence Lifetime Imaging ;

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Microscopy-related articles from our journals

HCR spectral imaging: 10-plex, quantitative, high-resolution RNA and protein imaging in highly autofluorescent samples Development 2024 151: dev202307
filoVision – using deep learning and tip markers to automate filopodia analysis J Cell Sci 2024 137: jcs261274
C omputational tools for quantifying actin filament numbers, lengths, and bundling Biology Open 2024 13: bio.060267

Microscopy-related preprint highlights

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