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Displaying posts in the category: Tools

Technology highlights - Spinning Disk Microscopy

Posted by , on 14 October 2020

Interview with Stoyno Stoynov, Ph.D. from the Center of Advanced Microscopy, Bulgarian Academy of Sciences, Bulgaria. Tell us a bit about the facility you run and what your focus is. The Bulgarian Node of Euro-Bioimaging ERIC is based in the Institute of Molecular Biology of the Bulgarian Academy of Sciences, Sofia. Our node is part

LSFM series – Surfing on the data freak wave! Part II: Before imaging: Know your sample (geometry)

Posted by , on 10 October 2020

This post, part of the blog series "LSFM series – Surfing on the data freak wave!", discusses (a) Sample preparation, (b) Light interaction with matter, (c) sample alignment and (d) checking fluorescence and calibration

Technology highlights – Structured Illumination Microscopy (SIM)

Posted by , on 30 September 2020

Interview with Ivan Novotný, Ph.D., from the Light Microscopy Core facility at the Institute of Molecular Genetics, in Prague, Czech Republic. Please tell us a bit about yourself and the facility where you work. I am employed as an imaging specialist at the Light Microscopy Core facility at the Institute of Molecular Genetics of the

LSFM series – Surfing on the data freak wave! PART I: Knowing your turf, knowing your surf!

Posted by , on 5 September 2020

Here we present a series of five blog posts with tips and tricks about light sheet microscopy. 1. The basics of LSFM (Sept 2020) 2. Improving sample mounting (Oct 2020) 3. Calibration and Acquisition (Nov 2020) 4. Tailoring the data (Dec 2020) 5. What is next? AI and smarter than us LSFM (Jan 2021)

SRRF-Stream+ Super-Resolution Microscopy Accessible to All

Sponsored by Andor, on 12 August 2020

Fast, reliable & live-cell compatible Super-Resolution Science has limits imposed by the laws of physics that constrain discoveries and the advance of knowledge. In microscopy, up until the beginning of the XXI century, the diffraction limit of light was an unbreakable barrier. This law of physics imposes that two points could not be resolved (clearly

“openFrame” for modular, extensible, easily maintained, open-source microscopy

Posted by , on 23 July 2020

The openFrame [1] is an open-source microscopy hardware project initiated by the Photonics Group in the Physics Department at Imperial College London that is intended to provide access to a modular, upgradable, easily maintained and modifiable microscope frame that can be implemented at relatively low cost compared to existing commercial instruments. openFrame is a component

How CLIJ2 can make your bio-image analysis workflows incredibly fast

Posted by , on 14 July 2020

Do you also spend a substantial amount of your time waiting for automated image analysis to finish? I did, again and again for more than a decade. And, then,  the morning after running a script overnight, you realize that a parameter was wrongly set. It was about time to change that. History Processing on graphics

Lessons learned from an open-hardware project in microscopy: The mesoSPIM initiative

Posted by , on 1 July 2020

The mesoscale selective plane illumination microscope (mesoSPIM) is a highly versatile open-source light-sheet microscope for imaging large (cm-sized) cleared tissue samples. Using the documentation and software available via mesospim.org and on Github, interested labs can set up their own microscope.  Currently, ten mesoSPIM instruments are in operation around the globe and several more are under