Displaying posts in the category: How to

The FocalPlane Network: joining and using the database

Posted by , on 20 September 2023

Our instructional videos take you step-by-step through the process of joining and using the FocalPlane Network. You can contact us at with any feedback on the Network. Joining the FocalPlane Network Using the FocalPlane Network

An interview with Helen Spiers

Posted by , on 18 September 2023

Citizen science is a fantastic way to engage the public with scientific research by getting them involved with real research projects. For scientists, the power of the crowd can help them overcome analysis bottlenecks that might otherwise slow the pace of their research. Martin Jones and Lucy Collinson, from the Crick Electron Microscopy Science Technology

All things unequal – live microscopy reveals asymmetric RNA localization in the early mammalian embryo

Posted by , on 4 September 2023

Authors: Jennifer Zenker1,*, Julian Heng2 1Australian Regenerative Medicine Institute (ARMI), Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton, Australia 2Remotely Consulting, Perth, Australia *Corresponding author Non-mammalian systems have been invaluable for us to decipher evolutionarily-conserved processes, orthologous molecules and cellular phenomena underlying early embryo development. One fundamental discovery from the work on non-mammalian

Sharing Your Poster on Figshare: A Community Guide to How-To and Why

Posted by , on 26 July 2023

Join the Open Science revolution and increase the impact of your research by sharing it on Figshare. Discover the benefits of transparency, collaboration, and accessibility in science as you make your work citable, shareable, and discoverable. Our step-by-step guide will walk you through uploading your conference posters and data, ensuring your research reaches a wider

Tracking in napari

Posted by , on 1 June 2023

In this blog post we are exploring cell tracking in napari. Two important processes in normal tissue development and disease are cell migration and proliferation. To gain a better understanding on these processes, tracking in time-lapse datasets is needed. By measuring track properties, like velocity and the total travelled distance, spatio-temporal relationships can be studied.

Feature extraction in napari

Posted by , on 3 May 2023

This blog post revolves around extracting and selecting features from segmented images. We will define the terms feature extraction and selection. Also, we will learn how to categorize features and can look up specific features in a glossary. Furthermore, we will explore how to extract features in napari. Definition of feature extraction During feature extraction,

Conference Tips from the yDGE

Posted by , on 26 April 2023

About yDGE DGE Young Microscopists, abbreviated yDGE, is a working group within the German Society for Electron Microscopy (DGE) for students, doctoral researchers, as well as early-career post-doctoral researchers and professionals. In January 2022, we started as a small (but all the more motivated) group of peers. Our vision was, and still is, to promote

Bypassing 164 years of tradition with 'any immersion microscopy'

Posted by , on 24 April 2023

The optical microscope is a classic scientific instrument with a straightforward purpose: to observe objects in more detail than is possible with the naked eye. Many microscope variations exist, from the rudimentary examples of the 17th century, to modern computer controlled systems with sophisticated designs. Despite the variety, most optical microscopes reuse the same physical

Quality assurance of segmentation results

Posted by , on 13 April 2023

This blog post revolves around determining and improving the quality of segmentation results. A common problem is that this step is often omitted and done rather by the appearance of the image segmentation than by actually quantifying it. This blogpost aims to show different ways to achieve this quantification as this leads to reproducibility. Therefore,

Annotating 3D images in napari

Posted by , on 30 March 2023

This blog post revolves around generating ground truth in 3D images for segmentation. Therefore, we will define what ground truth is and how we can generate it using napari in a time-efficient way. We will also learn about difficulties of annotating alone or in groups and address possible solutions for both. Challenges of image segmentation