Microscopy preprints – new tools and techniques in imaging

Posted by FocalPlane, on 20 September 2024

Here is a curated selection of preprints posted recently on new tools and techniques in imaging. Let us know if we are missing any recent preprints that are on your reading list!

In Vivo Optical Clearing of Mammalian Brain
Giovanni Talei Franzesi, Ishan Gupta, Ming Hu, Kiryl Piatkevich, Murat Yildirim, Jian-Ping Zhao, Minho Eom, Seungjae Han, Demian Park, Himashi Andaraarachchi, Zhaohan Li, Jesse Greenhagen, Amirul Muhammad Islam, Parth Vashishtha, Zahid Yaqoob, Nikita Pak, Alexander D Wissner-Gross, Daniel A. Martin-Alarcon, Jonathan J. Veinot, Peter T. C. So, Uwe Kortshagen, Young-Gyu Yoon, Mriganka Sur, Edward S. Boyden

Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo
Shigenori Inagaki, Nao Nakagawa-Tamagawa, Nathan Huynh, Yuki Kambe, Rei Yagasaki, Satoshi Manita, Satoshi Fujimoto, Takahiro Noda, Misato Mori, Aki Teranishi, Hikari Takeshima, Yuki Naitou, Tatsushi Yokoyama, Masayuki Sakamoto, Katsuhiko Hayashi, Kazuo Kitamura, Yoshiaki Tagawa, Satoru Okuda, Tatsuo K. Sato, Takeshi Imai

Figure extracted from Inagaki, *et al.* made available under a CC-BY-NC 4.0 International license.

Molecular recording of cellular protein kinase activity with chemical labeling
De-en Sun, Siu Wang Ng, Yu Zheng, Shu Xie, Niklas Schwan, Paula Breuer, Dirk C. Hoffmann, Julius Michel, Daniel D. Azorin, Kim E. Boonekamp, Frank Winkler, Wolfgang Wick, Michael Boutros, Yulong Li, Kai Johnsson

Genetically Encoded FerriTag as a Specific Label for Cryo-Electron Tomography
Chang Wang, Ioan Iacovache, Benoît Zuber

Figure extracted from Wang, *et al.* made available under a CC-BY 4.0 International license.

The eduWOSM: an advanced optical microscope for education
N.J. Carter, D.S. Martin, J.E. Molloy, R.A. Cross

Figure extracted from Carter, *et al.* made available under a CC-BY 4.0 International license.

Miniaturizing, Modifying, and Augmenting Nature’s Proteins with Raygun
Kapil Devkota, Daichi Shonai, Joey Mao, Scott Soderling, Rohit Singh

Tunable Cytosolic Chloride Indicators for Real-Time Chloride Imaging in Live Cells
Jared Morse, Maheshwara Reddy Nadiveedhi, Matthias Schmidt, Fung-Kit Tang, Colby Hladun, Prasanna Ganesh, Zhaozhu Qiu, Kaho Leung

Quantitative Comparison of Monomeric StayGold Variants Using Protein Nanocages in Living Cells
Giulia Viola, Kyle A. Jacobs, Joël Lemière, Matthew L. Kutys, Torsten Wittmann

Nanoscale volumetric fluorescence imaging via photochemical sectioning
Wei Wang, Xiongtao Ruan, Gaoxiang Liu, Daniel E. Milkie, Wenping Li, Eric Betzig, Srigokul Upadhyayula, Ruixuan Gao

Multi-camera Simultaneous Total Internal Reflection and Interference Reflection Microscopy
Jeffrey O. Spector, Jiayi Chen, Antonina Roll-Mecak

Implementation and calibration of spectroscopic single-molecule localization microscopy
Benjamin Brenner, Wei-Hong Yeo, Youngseop Lee, Junghun Kweon, Cheng Sun, Hao F. Zhang

Open-hardware solutions for light sheet microscopy specimen chambers
Artemiy Golden, Julien Colombelli, Ernst H.K. Stelzer, Francesco Pampaloni

Figure extracted from Golden, *et al.* made available under a CC-BY 4.0 International license.

Sidelobe suppressed Bessel beams for one-photon light-sheet microscopy
Chetna Taneja, Jerin Geogy George, Stella Corsetti, Philip Wijesinge, Graham D. Bruce, Maarten F. Zwart, Shanti Bhattacharya, Kishan Dholakia

Figure extracted from Taneja, *et al.* made available under a CC-BY 4.0 International license.

Single-shot impulsive stimulated Brillouin microscopy by tailored ultrashort pulses
David Krause, John Boehm, Leon Liebig, Nektarios Koukourakis, Juergen W. Czarske

Novel Genetically Encoded Biosensors for Functional Imaging of Cell Signaling by Polarization Microscopy
Paul Miclea, Vendula Nagy-Marková, Robin Van den Eynde, Wim Vandenberg, Alina Sakhi, Alexey Bondar, Peter Dedecker, Josef Lazar

Ultraplex microscopy: versatile highly-multiplexed molecular labeling and imaging across scale and resolution
Janeth Pérez-Garza, Jairo Orea, Zachary Deane, Gianna Raimondi, Rebecca Tripp, Imani Charles, Linnaea Ostroff

Event-based Single Molecule Localization Microscopy (eventSMLM) for High Spatio-Temporal Super-resolution Imaging
Jigmi Basumatary, S Aravinth, Neeraj Pant, Vignesh Ramanathan, Chetan Singh Thakur, Partha Pratim Mondal

One step 4x and 12x 3D-ExM: robust super-resolution microscopy in cell biology
Roshan X Norman, Yu-Chia Chen, Emma E Recchia, Jonathan Loi, Quincy Rosemarie, Sydney L Lesko, Smit Patel, Nathan Sherer, Motoki Takaku, Mark E Burkard, Aussie Suzuki

Figure extracted from Norman, *et al.* made available under a CC-BY-NC 4.0 International license.

A Cryo-to-Liquid Phase Correlative Light Electron Microscopy Workflow for the Visualization of Biological Processes in Graphene Liquid Cells
Luco Rutten, Ben Joosten, Judith Schaart, Marit de Beer, Rona Roverts, Steffen Gräber, Willi Jahnen-Dechent, Anat Akiva, Elena Macías-Sánchez, Nico Sommerdijk

A method to increase the imaging efficiency of tiling light sheet microscopy using scanning non-coaxial beam arrays synchronized with regional virtual confocal slits
Liang Gao

Super-Resolution Simplified: Sub-10nm Imaging Over Large Areas and Deep Penetration via SDC-OPR and DNA-PAINT
Cecilia Zaza, Megan D. Joseph, Olivia P. L. Dalby, Rhian F. Walther, Karol Kołątaj, Germán Chiarelli, Franck Pichaud, Guillermo P. Acuna, Sabrina Simoncelli

Figure extracted from Zaza, *et al.* made available under a CC-BY 4.0 International license.

Exploring transient states of PAmKate to enable improved cryogenic single-molecule imaging
Davis Perez, Dara P. Dowlatshahi, Christopher A. Azaldegui, T. Bertie Ansell, Peter D. Dahlberg, W. E. Moerner

ClearScope: a fully integrated light sheet theta microscope for sub-cellular resolution imaging without lateral size constraints
Matthew G. Fay, Peter J. Lang, David S. Denu, Nathan J. O’Connor, Benjamin Haydock, Jeffrey Blaisdell, Nicolas Roussel, Alissa Wilson, Sage M. Aronson, Paul J. Angstman, Cheng Gong, Tanvi Butola, Orrin Devinsky, Jayeeta Basu, Raju Tomer, Jacob R. Glaser

High-speed 4D fluorescence light field tomography of whole freely moving organisms
Kevin C. Zhou, Clare Cook, Archan Chakraborty, Jennifer Bagwell, Joakim Jönsson, Kyung Chul Lee, Xi Yang, Shiqi Xu, Ramana Balla, Mark Harfouche, Donald T. Fox, Michel Bagnat, Roarke Horstmeyer

Figure extracted from Zhou, *et al.* made available under a CC-BY 4.0 International license.

Reduction of SEM charging artefacts in native cryogenic biological samples
Abner Velazco, Thomas Glen, Sven Klumpe, Avery Pennington, Jianguo Zhang, Jake LR Smith, Calina Glynn, William Bowles, Maryna Kobylynska, Roland A. Fleck, James H. Naismith, Judy S Kim, Michele C. Darrow, Michael Grange, Angus I Kirkland, Maud Dumoux

Whole-cell multi-target single-molecule super-resolution imaging in 3D with microfluidics and a single-objective tilted light sheet
Nahima Saliba, Gabriella Gagliano, Anna-Karin Gustavsson

SNAP-tag2: faster and brighter protein labeling
Stefanie Kühn, Veselin Nasufovic, Jonas Wilhelm, Julian Kompa, Eline M.F. de Lange, Yin-Hsi Lin, Cornelia Egoldt, Jonas Fischer, Artem Lennoi, Miroslaw Tarnawski, Jochen Reinstein, Rifka Vlijm, Julien Hiblot, Kai Johnsson