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FocalPlane

Posts from the FocalPlane team

About FocalPlane

Microscopy background: Optical System Development, Image Analysis, Probe Development

Posts by FocalPlane

Featured image with Nick Gatford

Posted by , on 16 November 2023

Our featured image shows a single human dopaminergic neuron generated from a human stem cell, acquired via super-resolution Airyscan confocal microscopy at the University of Oxford Micron facility by Nick Gatford. We caught up with Nick to find out about his research and what he is excited about in microscopy.

Featured image with Oona Paavolainen

Posted by , on 15 November 2023

Our featured image, acquired by Oona Paavolainen, shows a primary mammary gland organoid, in a collagen-I matrix, formed from a single primary epithelial cell isolated from material obtained from a breast reduction surgery. The image was taken with a spinning disk confocal microscope (3i CSU-W1 Spinning disk) using a 20x Zeiss Plan-Apochromat (NA=0.8) objective. The

How to Minimise Photodamage with LED illumination

Sponsored by CoolLED, on 15 November 2023

Both fixed and live samples are susceptible to photodamage during fluorescence microscopy, which can shorten time-lapse experiments and cast doubt on observations. LED illumination for widefield fluorescence has the potential to counter this serious problem, whether running a complete experiment or simply finding the region of interest prior to confocal microscopy. Exposing biological samples to

Featured image with Till Stephan

Posted by , on 14 November 2023

Our featured image, acquired by Till Stephan, shows a live-cell stimulated emission depletion (STED) microscopy recording of a HeLa cell in which mitochondria (orange-magenta) and microtubules (cyan) are labelled. The super-resolution image reveals the densely stacked mitochondrial cristae, coordinated invaginations of the mitochondrial inner membrane.

New look for FocalPlane

Posted by , on 13 November 2023

We are delighted to launch a new look for FocalPlane. Every day this week, we’ll be releasing a new banner image and highlighting the scientist that acquired it. Today, we start with an image from Rebecca Simkin. Rebecca won our 2023 image competition, and you can read our featured image post with Rebecca here: https://focalplane.biologists.com/2023/09/01/and-the-winner-is/

Recent events by FocalPlane

Analytical Microscopy Meeting

Abstract submission deadline: 29th September 2025

Location: London , United Kingdom

Date: 26 February 2026 — 27 February 2026

Live cell imaging

Location: Prague, Czech Republic

Date: 3 December 2025 — 5 December 2025

Mechanical characterization of biological samples using correlative methods

Location: Prague, Czech Republic

Date: 25 November 2025 — 26 November 2025