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Microscopy Open Access collection 2020

Posted by , on 19 October 2020

Here on FocalPlane, Open Access Week is especially close to our hearts as it coincides with the birthday of the father of microscopy, Antonie van Leeuwenhoek (24 October). So every year for Open Access Week, we will be listing all the microscopy-related Open Access articles from The Company of Biologists journals on FocalPlane under the

Technology highlights - Spinning Disk Microscopy

Posted by , on 14 October 2020

Interview with Stoyno Stoynov, Ph.D. from the Center of Advanced Microscopy, Bulgarian Academy of Sciences, Bulgaria. Tell us a bit about the facility you run and what your focus is. The Bulgarian Node of Euro-Bioimaging ERIC is based in the Institute of Molecular Biology of the Bulgarian Academy of Sciences, Sofia. Our node is part

How I got into the Flamingo project, a new way of sharing custom-built light sheet microscopes

Posted by , on 12 October 2020

Sharing microscopy technology is crucial for biomedical research. This is the story how I joined Jan Huisken and his team to give other scientists quick access to custom-built light sheet microscopes.

LSFM series – Surfing on the data freak wave! Part II: Before imaging: Know your sample (geometry)

Posted by , on 10 October 2020

This post, part of the blog series "LSFM series – Surfing on the data freak wave!", discusses (a) Sample preparation, (b) Light interaction with matter, (c) sample alignment and (d) checking fluorescence and calibration

FocalPlane image competition voting is open!

Posted by , on 5 October 2020

After 3 weeks of submissions we are happy to announce that the submissions gallery is now up and running for 1 week (until 12 October, 5pm UK time)! We have 83 fantastic entries from 45 different people who have used a range of different microscopy techniques to acquire their beautiful images. To see our competition

Technology highlights – Structured Illumination Microscopy (SIM)

Posted by , on 30 September 2020

Interview with Ivan Novotný, Ph.D., from the Light Microscopy Core facility at the Institute of Molecular Genetics, in Prague, Czech Republic. Please tell us a bit about yourself and the facility where you work. I am employed as an imaging specialist at the Light Microscopy Core facility at the Institute of Molecular Genetics of the

Bioimage Analysis in FIJI - Resource List

Posted by , on 14 September 2020

If you are on this site, you might be aware of some of the open source image processing and analysis tools are available to you. The toolbox in this space is rapidly expanding. But that doesn’t always mean it’s easy to navigate – it can actually be quite daunting. Luckily the bio-imaging community is friendly

FocalPlane image competition

Posted by , on 9 September 2020

For our first FocalPlane image competition we are delighted to invite our community (you!) to submit your favourite images that you have acquired using a microscope of any modality. The images you submit (up to 3 images per applicant) will be featured on FocalPlane and will be voted on by the community. The image with

‘Under the scope’: an interview with Ann Wheeler

Posted by , on 8 September 2020

Ann Wheeler received her PhD from University College London as part of a molecular cell biology 4-year rotation programme where she got to use confocal imaging quite extensively. She then moved to Columbia University to do some total internal reflection fluorescence (TIRF) microscopy. She was actually one of the first people to use TIRF as

LSFM series – Surfing on the data freak wave! PART I: Knowing your turf, knowing your surf!

Posted by , on 5 September 2020

Here we present a series of five blog posts with tips and tricks about light sheet microscopy. 1. The basics of LSFM (Sept 2020) 2. Improving sample mounting (Oct 2020) 3. Calibration and Acquisition (Nov 2020) 4. Tailoring the data (Dec 2020) 5. What is next? AI and smarter than us LSFM (Jan 2021)