FocalPlane

Posts from the FocalPlane team

Featured image with Jovan Brockett

Posted by FocalPlane, on 19 July 2024

Our featured image, acquired by Jovan Brockett, shows a Drosophila melanogaster embryo undergoing syncytial nuclear divisions during early embryonic development. It was prepared using PFA fixation and hand dissected to remove the outer vitelline membrane. The image was captured using a Nikon Ti-E system fitted with a Yokagawa CSU-X1 spinning disk head, Hamamatsu Orca Flash

Featured image with Hoang Anh Le

Posted by FocalPlane, on 5 July 2024

Our featured image, acquired by Hoang Anh Le, shows an Ewing’s sarcoma cancer cell plated on a fibronectin-coated surface. The cell was fixed with 4% formaldehyde and stained using phalloidin (white) and DAPI (in pink). The sample was imaged using a confocal Zeiss 880 microscope with Airyscan.

Featured image with Theresa Wiesner

Posted by FocalPlane, on 21 June 2024

Our featured image, acquired by Theresa Wiesner, shows a neuron in dissociated hippocampal culture and was imaged using highly inclined thin illumination microscopy (HiLO). The neuron is transfected with a neurotransmitter (glutamate) sensor and shown in orange. The image was acquired using Nikon Elements software / microscope and post-processing was done using FIJI (ImageJ). Find