Our lab develops, improves and compares genetically encoded fluorescent probes, that are inspired by Green Fluorescent Protein (GFP). We use the most optimal fluorescent proteins to generate biosensors for quantitative functional imaging with the overarching goal to unravel (G protein) signaling networks in time and space in cells and tissues. The majority of the genetically encoded tools that we have developped are shared through addgene. To simplify the quantification and presentation of data, we develop analysis tools and data visualization apps that are gathered here.
Scientific field: Cell biology, Molecular biology, Biophysics, Biochemistry, Biotechnology
Microscopy background: Probe Development, Image Analysis
Posted by Joachim Goedhart, on 4 October 2022In talks about fluorescent proteins I usually include a timeline of events related to Green Fluorescent Protein (GFP). The timeline highlights some of the key moments in the history of fluorescent protein discovery and engineering. I am generally fond of timelines, since they provide a way to pay tribute to the pioneers, and other researchers
Posted by Joachim Goedhart, on 7 July 2020I first crossed paths with green fluorescent protein (GFP) while doing an internship in 1996 at the Max Planck Institute in Göttingen. I was chemically labeling epidermal growth factor (EGF) with the fluorescent dyes Cy3 and Cy5 to study growth-factor signaling. Two post-docs were discussing the use of GFP as an alternative fluorescent tag and