Shoh Asano received his PhD in Prof. Wolfgang Baumeister’s group at the Max-Planck Institute of Biochemistry in Munich (Germany). During that time, his major contributions included novel applications of cryo-electron tomography, enabling the first in-situ detection of single protein complexes in neurons without any staining. He proceeded to join Prof. Ed Boyden’s Synthetic Neurobiology group at MIT as a postdoctoral associate. In that role, he began developing and applying expansion microscopy concepts across many imaging modalities and species. One of the projects was an exciting collaboration with his colleague and friend Rui together with Prof. Eric Betzig from HHMI Janelia Research Campus, which enabled imaging of an expanded entire fly brain with a lattice light-sheet microscope and offering unprecedented imaging at scale. Shoh now works at Pfizer as a Principal Scientist.
Microscopy background: Optical System Development, Image Analysis
Posted by Shoh Asano, on 29 July 2020Written by Shoh Asano and Ruixuan Gao Light microscopy and diffraction limit For centuries, light microscopy has played a central role in biological studies. The first implementations of a light microscope dates back to as early as the late 16th and early 17th century, when an array of polished lenses was used to magnify (biological)