Sravasti Mukherjee

Currently a PhD student in the group of Kees Jalink(NKI, Amsterdam), working on developing a high content imaging based screening platform. I use FRET-FLIM and optogenetic systems to study the dynamics of signaling pathways, at single cell resolution.

Behind the Screen - behind the scenes of setting up a dynamic screening platform

Posted by Sravasti Mukherjee, on 22 November 2021

The Cell Biophysics lab has focused on FRET (Forster Resonance Energy Transfer) as it is a powerful technique to investigate dynamic protein-protein interactions, along with studying almost every aspect of cellular signaling with biosensors. FRET is detected either by ratioing the  intensities of the FRET donor and acceptor,  or by FLIM (Fluorescence Lifetime Imaging ;