We are excited to announce our new webinar series FocalPlane features…, covering all aspects of microscopy, from technology development to cell biology applications to image analysis.
On the first Tuesday of every month, an invited speaker will talk about their research involving microscopy. The talks will be hosted in the platform Remo, our browser-based conferencing platform (read our guide to using Remo). Following the Q&A session, we will host a more informal session especially designed for early-career researchers to meet the speaker. In order to facilitate the conversation, a maximum of 7 people will meet with the speaker at a time. During this session, we will have two more ‘tables’ available: one for FocalPlane, hosted by Esperanza Agullo-Pascual, and another for Journal of Cell Science Editors, so you will also have the chance to meet and speak with us if you would like to.
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Follicular Helper T cells, Cytotoxic T cells (CTL) and Natural Killer (NK) cells are effectors of the immune system that play important roles in defense against viral infection and cancer. In this talk I will present three vignettes related to super-resolution exploration of effector immunological synapses with relevance to infection and cancer.
Early studies of the immunological synapse revealed a canonical architecture with a striking accumulation of T cell receptor (TCR) in the center driven by actin cytoskeleton and endosomal sorting complexes required for transport (ESCRT). The ESCRT machinery can mediate in situ conversion of TCR microclusters into extracellular vesicles we refer to as synaptic ectosomes. We have shown that one function of synaptic ectosomes is the provision of highly active CD40 ligand clusters, which is a key effector molecule of helper T cells. Super-resolution imaging revealed the possibility of recognition and effector subdomains in synaptic ectosomes.
The second topic is the new discovery of cytotoxic bombs deployed by CTL and NK cells against targets. CTL and NK cells assemble cytotoxic proteins into supramolecular attack particles (SMAPs) with a core of perforin and granzymes and a shell of thrombospondin-1. Super-resolution dSTORM imaging again revealed that
The third topic relates to the role of the adhesion molecule CD2- which mediates “liquid” adhesions. I will describe how CD2, the receptor for CD58 on T cells, contributes to signal amplification in T cells. CD2 expression is often low in “exhausted” CD8 tumor infiltrating lymphocytes in several types of cancer, although the regulation of CD2 seems to be distinct from other exhaustion associated receptors like PD1. The CD2-CD58 corolla recruits CD28 and PD1 interactions, but super-resolution imaging also suggests nanoscale subdomains.
In summary, the super-resolution microscopy analysis of immunological synapses with supported lipid bilayers continues to generate insights into T cell effector mechanisms.
If you missed FocalPlane features… you can still watch the recorded webinars.
6 July 2021, 5pm BST – Eva Nogales, University of California, Berkeley, USA.
4 May 2021, 2PM BST – Jennifer Lippincott-Schwartz, Janelia Research Campus, HHMI, Ashburn, VA.
How to use Remo
Joining the seminar
The conference centre will open five minutes before the start of the webinar and we recommend using the Chrome browser to take part.
To join, first click the link in the email you’ll receive on the day of the webinar, then register or log in to Remo and click ‘Join Event’. Please allow access to your webcam and microphone and carry out the system check before joining. Passing these checks avoids technical problems during the discussion session. Note that your webcam and microphone will not be shared during the presentations.
Once you enter the conference centre, you’ll see some introductory slides, which will play (without sound) until the FocalPlane Community Manager introduces the session. During this time, you can create your profile – add a photo and some personal information if you want. You can also put any questions to the organisers via the chat. If you want to leave the event at any point, either click ‘Quit Event’ or simply close your browser window.
Watching the webinar
The webinar will begin after the FocalPlane Community Manager introductory remarks. Please use the Q&A tab to ask the speaker questions during or at the end of the talk. After the talk, the Chair will host a live Q&A session using these questions. For a more interactive discussion with the speaker, we encourage you to use the ‘Raise Hand’ icon if you would like to ask your question directly to the speaker. The Chair will bring you to the stage and your camera and microphone will turn on for you to speak. You can also upvote other participants’ questions in the Q&A tab by clicking the up arrow on the individual question.
Meet-the-speaker session and virtual networking
Once the Q&A finishes, you’ll be transferred into the Remo conference centre, which will be open for an hour or so to give you the chance to meet the speaker, the FocalPlane Community Manager and the Journal of Cell Science team, as well as other participants.
You’ll find yourself on a table with up to seven other people – you’ll only see the video feeds of everyone on the table (Remo works best if camera and mic are switched on). If you want to move tables, simply double click on the table you want to go to. Be aware that new people can drop into your table at any time unless the table is full. You’ll hear a ‘knock, knock’ noise when someone is joining your table.
You’ll also find the lift on the left of the screen, which you can use to move between floors. The ‘Meet the speaker’ table as well as FocalPlane and ‘Meet the Editors’ tables will be located on the first floor.
Remember that the ‘meet the speaker’ session is especially designed for early-career researchers, so please be considerate and let them join first the speaker’s table. The table has only 7 seats available in order to facilitate the conversation of the group. Depending on the number of participants willing to meet the speaker, we will set rounds of 10-15 minutes with the speaker. Once the time is over, an announcement will pop-up in Remo asking the participants at the table to leave their spot. Please move to a different table and let other participants join the speaker’s table.
The majority of issues can be solved by refreshing your browser. Have you given permission for your browser to use your camera and mic? You can always ask for help during the session in the Chat, or email us afterwards at firstname.lastname@example.org to let us know about any problems you weren’t able to resolve.
Banner image credits
COS cells, super-resolution microscopy – Christophe Leterrier
Breast xenograft tumor tissue, two-photon microscopy – Battuya Bayarmagnai
Bacterium cryo-electron tomogram segmentation – Kanika Khanna
Solanum citrullifolium flower, scanning electron microscopy – Casper van der Kooi
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