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Our featured image shows the full morphology of Relaxin-3 neurons in an entire adult mouse brain. The neurons of interest are expressing GFP after viral injections in a pontine nucleus called nucleus incertus. To observe the full depth of this brain, it was made transparent by applying an optical clearing technique called Adipoclear. This method is a variant of IDISCO+, based on organic solvents. The sample was observed on an Ultramicroscope II light sheet microscope, using a 4x magnification. The image is a maximum intensity projection over the entire depth, created in FIJI.
The sample was prepared by Thibault Dhellemmes and imaged by Jérémie Teillon.

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Updated on 1 March 2024