Ask Erin / Dear Beth

On Ask Erin/Dear Beth, bioimage analysis experts Beth Cimini and Erin Weisbart, of the Imaging Platform at the Broad Institute of MIT and Harvard, answer your image analysis questions! Whether it’s about tuning or troubleshooting, segmenting or measuring, Erin and Beth have opinions and by golly are they going to share them!

Using mostly CellProfiler, but with expertise in many other image analysis softwares such as ImageJ/Fiji, Cellpose, Piximi, and more, no image analysis question is too hard, so ask away!

Submit your own questions at broad.io/AskErinDearBeth Images and pipelines we’ve used are available for you to download and play with at https://ciminilab.github.io/AskErin_DearBeth/

Ask Erin/Dear Beth is partially funded by grants from both the NIH (P41 GM135019) and CZI (2023-329649) to BAC – but our funders aren’t responsible for any of the cursing!