Andrey Andreev, Desiderio AscencioCalifornia Institute of Technology, David Prober labaandreev@caltech.edu Calcium imaging is a widely used tool in neuroscience, and our community now has access to multiple computational tools to process and analyze these data. However, a significant portion of work with imaging data relies on much simpler approaches than offered in these software packages.
Technological advancement constantly makes these methods more accessible, however, there are a number of understated complexities involved with these types of imaging-based experiments