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Displaying posts in the category: How to

Teaching co-localisation analysis from lecture to leisure

Posted by , on 20 October 2024

The origin There was one thing I was always skeptical about: colocalization analysis. When I started out as a BioImage Analyst, it was one of the first things I had to help users with in the BIOP at EPFL. I started learning more about it (and still continue to do so), tried with my own

FocalPlane features... data management

Posted by , on 23 September 2024

The fourth webinar in the latest series of FocalPlane features… was on data management. Caterina Strambio De Castillia introduced us to the topic of research data management, while Tom discussed how to use OMERO from the perspective of a group leader, a microscopist and an image analyst. We wrapped up the webinar with a short

MicroList and FocalPlane join forces

Posted by , on 2 September 2024

MicroList’s database of resources are now available on FocalPlane. The resources, which have been created by the global imaging community, are presented as cards that are divided into community resources, education resources and tools. The database is fully searchable using our search bar or can be filtered using a variety of categories.

DL4MicEverywhere - Overcoming reproducibility challenges in deep learning microscopy imaging

Posted by , on 29 July 2024

DL4MicEverywhere is out! Learn about it and how you can apply deep learning workflows on microscopy imaging in reproducible research and in a user-friendly manner across different systems.

Prompt Engineering in Bio-image Analysis

Posted by , on 18 July 2024

Prompt engineering and its importance Communication is key. This saying is not only true in day-to-day life, but also when interacting with Generative Artificial Intelligence, a system able to generate text, images or other output types in response to prompts. In prompt engineering, we use natural language to describe the task that a Large Language

How to write a bug report

Posted by , on 3 April 2024

Imagine you try out a software tool for the analysis of your data and for some reason it is not working in the way you expected. Neither the user documentation, nor forums like image.sc or stack overflow can provide answers. Then it is likely that you either found a bug in the software tool or

FocalPlane features... microscopy-based outreach

Posted by , on 11 March 2024

The first webinar of our new series of FocalPlane features... focussed on microscopy-based outreach. We hosted fantastic talks from Paola Moreno-Roman from Foldscope Instruments and Martin Jones from The Francis Crick Institute and we share the recordings here.

New FocalPlane features... webinar series

Posted by , on 22 February 2024

We are excited to announce a new series of FocalPlane features… webinars. Our first webinar, on 7 March at 15:00 GMT (10:00 PET) focusses on outreach projects using microscopy, with talks from Paola Moreno-Roman, Alexander Strachan and Martin Jones.

How to Minimise Photodamage with LED illumination

Sponsored by CoolLED, on 15 November 2023

Both fixed and live samples are susceptible to photodamage during fluorescence microscopy, which can shorten time-lapse experiments and cast doubt on observations. LED illumination for widefield fluorescence has the potential to counter this serious problem, whether running a complete experiment or simply finding the region of interest prior to confocal microscopy. Exposing biological samples to

Analyzing calcium imaging data using Python

Posted by , on 27 October 2023

Andrey AndreevCalifornia Institute of Technology, David Prober labaandreev@caltech.edu Calcium imaging allows tracking neural activity in time with single-cell resolution. There are many questions you might be interested in answering using this technique: which cells are tuned to the stimuli? is there periodic activity present in the data? which cells act together? how complex is the activity