Displaying posts with the tag: is_archive

Using Nile Blue and Nile Red to visualise the presence of lipids in cryosectioned tissues

Posted by , on 14 September 2021

Lipids are crucial elements of mammalian (and non-mammalian) cell biology and yet lipids are challenging to visualise in situ. In comparison to proteins, which we can generate antibodies for, or carbohydrates, some of which we can detect using fluorescent lectins, there are relatively few lipid-specific fluorescent probes. Many lipids are highly conserved across species making

Considerations for expression of fluorescent proteins and imaging in mammalian cells

Posted by , on 7 July 2021

Introduction to fluorescent proteins  Fluorescent proteins have the property of absorbing light at one wavelength and emit light in a longer wavelength. These proteins were observed first in bioluminescent organisms known to humanity for centuries. We can find examples of light-emitting organisms in multiple taxa: from single cell organisms like bacteria, to vertebrates like fish.