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FocalPlane

Posts from the FocalPlane team

About FocalPlane

Microscopy background: Optical System Development, Image Analysis, Probe Development

Posts by FocalPlane

Featured image with Oona Paavolainen

Posted by , on 15 November 2023

Our featured image, acquired by Oona Paavolainen, shows a primary mammary gland organoid, in a collagen-I matrix, formed from a single primary epithelial cell isolated from material obtained from a breast reduction surgery. The image was taken with a spinning disk confocal microscope (3i CSU-W1 Spinning disk) using a 20x Zeiss Plan-Apochromat (NA=0.8) objective. The

How to Minimise Photodamage with LED illumination

Sponsored by CoolLED, on 15 November 2023

Both fixed and live samples are susceptible to photodamage during fluorescence microscopy, which can shorten time-lapse experiments and cast doubt on observations. LED illumination for widefield fluorescence has the potential to counter this serious problem, whether running a complete experiment or simply finding the region of interest prior to confocal microscopy. Exposing biological samples to

Featured image with Till Stephan

Posted by , on 14 November 2023

Our featured image, acquired by Till Stephan, shows a live-cell stimulated emission depletion (STED) microscopy recording of a HeLa cell in which mitochondria (orange-magenta) and microtubules (cyan) are labelled. The super-resolution image reveals the densely stacked mitochondrial cristae, coordinated invaginations of the mitochondrial inner membrane.

New look for FocalPlane

Posted by , on 13 November 2023

We are delighted to launch a new look for FocalPlane. Every day this week, we’ll be releasing a new banner image and highlighting the scientist that acquired it. Today, we start with an image from Rebecca Simkin. Rebecca won our 2023 image competition, and you can read our featured image post with Rebecca here: https://focalplane.biologists.com/2023/09/01/and-the-winner-is/

Imaging with the Microscopy and BioImaging Facility at the Leloir Institute Foundation, Buenos Aires, Argentina.

Posted by , on 9 November 2023

In our ‘Imaging with…’ blog post, we meet the staff of the Microscopy and BioImaging Facility at the Leloir Institute Foundation, Buenos Aires.

Recent events by FocalPlane

International conference on Imaging and Characterisation for a Sustainable World ICWORLD 2027

Abstract submission deadline: 31st July 2026

Early registration deadline: 30th September 2026

Location: Oulu, Finland

Date: 13 January 2027 — 15 January 2027

AI and computational biology

Abstract submission deadline: 7th October 2026

Early registration deadline: 28th October 2026

Location: Leuven, Belgium

Date: 9 December 2026 — 10 December 2026

Processing and Analysis of Biological Images

Location: Brno, Czech Republic

Date: 1 December 2026 — 4 December 2026