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Displaying posts in the category: How to

behind DeXtrusion: an automated pipeline to detect epithelial cell extrusion through machine learning

Posted by Alexis Villars, on 6 October 2023

The problem In animals, epithelial tissue covers most of our organs. In order to create the very intricate and complex shapes of these organs, these tissues are drastically remodelled during embryogenesis through growth and shape changes. Once they achieve these final shapes, they maintain their cell number in a dynamic equilibrium. Both of these mechanisms,

How to… Publish Images blog series

Posted by FocalPlane, on 27 September 2023

by Helena Jambor In biology, images document appearances or structures of animals, issues, or cells, and increasingly are a source of quantitative insights of biological processes. Many papers are published every single day with images (Lee, 2018).Not all of these images are truthful or insightful. Some images are manipulated to mislead audiences (Bik et al.,

The FocalPlane Network: joining and using the database

Posted by FocalPlane, on 20 September 2023

Our instructional videos take you step-by-step through the process of joining and using the FocalPlane Network. You can contact us at focalplane@biologists.com with any feedback on the Network. Joining the FocalPlane Network Using the FocalPlane Network

An interview with Helen Spiers

Posted by Helen Zenner, on 18 September 2023

Citizen science is a fantastic way to engage the public with scientific research by getting them involved with real research projects. For scientists, the power of the crowd can help them overcome analysis bottlenecks that might otherwise slow the pace of their research. Martin Jones and Lucy Collinson, from the Crick Electron Microscopy Science Technology

All things unequal – live microscopy reveals asymmetric RNA localization in the early mammalian embryo

Posted by Jennifer Zenker, on 4 September 2023

Authors: Jennifer Zenker1,*, Julian Heng2 1Australian Regenerative Medicine Institute (ARMI), Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton, Australia 2Remotely Consulting, Perth, Australia *Corresponding author Non-mammalian systems have been invaluable for us to decipher evolutionarily-conserved processes, orthologous molecules and cellular phenomena underlying early embryo development. One fundamental discovery from the work on non-mammalian

Sharing Your Poster on Figshare: A Community Guide to How-To and Why

Posted by Elisabeth Kugler, on 26 July 2023

Join the Open Science revolution and increase the impact of your research by sharing it on Figshare. Discover the benefits of transparency, collaboration, and accessibility in science as you make your work citable, shareable, and discoverable. Our step-by-step guide will walk you through uploading your conference posters and data, ensuring your research reaches a wider

Tracking in napari

Posted by Mara Lampert, on 1 June 2023

In this blog post we are exploring cell tracking in napari. Two important processes in normal tissue development and disease are cell migration and proliferation. To gain a better understanding on these processes, tracking in time-lapse datasets is needed. By measuring track properties, like velocity and the total travelled distance, spatio-temporal relationships can be studied.

Feature extraction in napari

Posted by Mara Lampert, on 3 May 2023

This blog post revolves around extracting and selecting features from segmented images. We will define the terms feature extraction and selection. Also, we will learn how to categorize features and can look up specific features in a glossary. Furthermore, we will explore how to extract features in napari. Definition of feature extraction During feature extraction,

Conference Tips from the yDGE

Posted by yDGE, on 26 April 2023

About yDGE DGE Young Microscopists, abbreviated yDGE, is a working group within the German Society for Electron Microscopy (DGE) for students, doctoral researchers, as well as early-career post-doctoral researchers and professionals. In January 2022, we started as a small (but all the more motivated) group of peers. Our vision was, and still is, to promote

Bypassing 164 years of tradition with 'any immersion microscopy'

Posted by Alfred Millett-Sikking, on 24 April 2023

The optical microscope is a classic scientific instrument with a straightforward purpose: to observe objects in more detail than is possible with the naked eye. Many microscope variations exist, from the rudimentary examples of the 17th century, to modern computer controlled systems with sophisticated designs. Despite the variety, most optical microscopes reuse the same physical

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Microscopy-related articles from our journals

HCR spectral imaging: 10-plex, quantitative, high-resolution RNA and protein imaging in highly autofluorescent samples Development 2024 151: dev202307
filoVision – using deep learning and tip markers to automate filopodia analysis J Cell Sci 2024 137: jcs261274
C omputational tools for quantifying actin filament numbers, lengths, and bundling Biology Open 2024 13: bio.060267

Microscopy-related preprint highlights

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