Confocal Microscopy - FocalPlane

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Microscopy preprints – applications in biology

Posted by FocalPlane, on 23 January 2026

Here is a curated selection of preprints published recently. In this post, we share preprints that use microscopy tools to answer questions in biology.

Imaging spotlight: Initiation of chromosome congression deconstructed by multi-scale live lattice light-sheet and super-resolution microscopy

Posted by Kruno Vukušić, on 16 January 2026

In this highlight, Kruno Vukušić and Iva M. Tolić describe how they use multi-scale live lattice light-sheet and super-resolution microscopy to decipher the earliest steps of chromosome congression.

Featured image with Ivan Radin

Posted by FocalPlane, on 16 January 2026

Our featured image, acquired by Ivan Radin, shows a few epidermal cells of tobacco (Nicotiana benthamiana) leaf, expressing cytosolic GFP. There is a significant variability in expression levels between cells. The brightest region is the nucleus, which is surrounded by a large central vacuole. The cytosol is restricted to the cell periphery. Transvacuolar strands connect

Featured image with Christina Daly

Posted by FocalPlane, on 6 January 2026

Our featured image, acquired by Christina Daly, is of an E9.5 murine neural tube. The embryo was fixed in 4% Paraformaldehyde, embedded in agarose, and vibratome-sectioned at 100 mm thickness. The sections were stained with for F-actin with phalloidin and imaged on a Nikon A1R confocal.

Vote for your favourite 'Featured image' from 2025

Posted by FocalPlane, on 19 December 2025

Throughout 2025, we highlighted some fantastic images and researchers in our 'Featured image' series. Here is your chance to vote for your favourite.

Featured image with Kaiden Power

Posted by FocalPlane, on 26 September 2025

Our featured image, acquired by Kaiden Power, shows the head and probolae of an Acrobeles complexus nematode. The cuticle furrows (annuli) and cilia pores are stained with Concanavalin A (red). The sample was mounted and imaged live on a DeltaVision widefield microscope with deconvolution post-acquisition.

Featured image with David Grainger

Posted by FocalPlane, on 29 August 2025

Our featured image, acquired by David Grainger, is a colour-coded depth projection of the blood endothelial cells of the E14.5 mouse embryonic thymus and surrounding structures. A 200 μm-thick vibratome section was immunostained for endomucin and imaged on a Zeiss LSM980 confocal microscope and depth-encoded using a rainbow LUT before performing a maximum intensity projection.

Featured image with Seth Agyei Domfeh

Posted by FocalPlane, on 20 June 2025

Our featured image, acquired by Seth Agyei Domfeh, shows the nuclear localisation of interferon regulatory factor 9 (IRF9) in human hepatoma (HepG2) cells stimulated with interferon α (IFN-α). HepG2 cells were cultured on a poly-D-lysine-coated coverslip for 24 hours and stimulated with 1000 IU/ml IFN-α. At 24 hours post-stimulation, the cells were fixed using 4%

Featured image with Rohit Nautiyal

Posted by FocalPlane, on 9 May 2025

Our featured image, acquired by Rohit Nautiyal, shows a monolayer of brain endothelial cells seeded on a glass substrate coated with fibronectin. The cells were fixed and stained for ZO-1 (LUT- Red Hot) to visualize the tight junctions and actin (LUT-blue). The image was captured with a spinning disk confocal microscope and post-processed with Fiji

A journey of a novice in immunofluorescence assay

Posted by Seth Domfeh, on 15 November 2024

I am Seth Domfeh, a lecturer and an early-career researcher from the Department of Biochemistry and Biotechnology, Kwame Nkrumah University of Science and Technology, Ghana. I am delighted to share my experience before and during my research visit to the Africa Microscopy Initiative (AMI) Imaging Centre, Institute of Infectious Disease and Molecular Medicine, University of

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Microscopy-related articles from our journals

Toggle-Untoggle – a cell segmentation tool with an interactive user verification interface J Cell Sci 2025 138: jcs264154
The ratio of Wnt signaling activity to Sox2 transcription factor levels predicts neuromesodermal fate potential Development 2025 152: dev204661
The short isoform of Tango1 is dispensable for zebrafish survival but is required for skeletal patterning and integrity Biology Open 2025 14: bio062117

Microscopy-related preprint highlights

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Microscopy-related articles from our journals

Microscopy-related preprint highlights

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