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Featured image with Denise Scuffi

Posted by FocalPlane, on 30 January 2026

Our featured image, acquired by Denise Scuffi, shows stomata – small pores surrounded by a pair of specialised cells known as guard cells – from Arabidopsis thaliana expressing the fluorescent biosensor roGFP2-Orp1 in the cytosol, which enables in vivo monitoring of intracellular H₂O₂ dynamics.

Featured image with Ivan Radin

Posted by FocalPlane, on 16 January 2026

Our featured image, acquired by Ivan Radin, shows a few epidermal cells of tobacco (Nicotiana benthamiana) leaf, expressing cytosolic GFP. There is a significant variability in expression levels between cells. The brightest region is the nucleus, which is surrounded by a large central vacuole. The cytosol is restricted to the cell periphery. Transvacuolar strands connect

Featured image with Christina Daly

Posted by FocalPlane, on 6 January 2026

Our featured image, acquired by Christina Daly, is of an E9.5 murine neural tube. The embryo was fixed in 4% Paraformaldehyde, embedded in agarose, and vibratome-sectioned at 100 mm thickness. The sections were stained with for F-actin with phalloidin and imaged on a Nikon A1R confocal.

Vote for your favourite 'Featured image' from 2025

Posted by FocalPlane, on 19 December 2025

Throughout 2025, we highlighted some fantastic images and researchers in our 'Featured image' series. Here is your chance to vote for your favourite.

Featured image with Aswathy G Krishnan

Posted by FocalPlane, on 10 October 2025

Our featured image, acquired by Aswathy G Krishnan, depicts a neural rosette of a cerebral organoid derived from human induced pluripotent stem cells (hiPSC). The organoids were collected, fixed, dehydrated, embedded in tissue freezing media and cryosectioned at 20 µm thickness. Then, the sections were immunolabelled and imaged under confocal microscope at 60X magnification. The

Featured image with Kaiden Power

Posted by FocalPlane, on 26 September 2025

Our featured image, acquired by Kaiden Power, shows the head and probolae of an Acrobeles complexus nematode. The cuticle furrows (annuli) and cilia pores are stained with Concanavalin A (red). The sample was mounted and imaged live on a DeltaVision widefield microscope with deconvolution post-acquisition.

Featured image with David Grainger

Posted by FocalPlane, on 29 August 2025

Our featured image, acquired by David Grainger, is a colour-coded depth projection of the blood endothelial cells of the E14.5 mouse embryonic thymus and surrounding structures. A 200 μm-thick vibratome section was immunostained for endomucin and imaged on a Zeiss LSM980 confocal microscope and depth-encoded using a rainbow LUT before performing a maximum intensity projection.

Featured image with Ludovica Altieri

Posted by FocalPlane, on 15 August 2025

Our featured image, acquired by Ludovica Altieri, shows murine primary cortical neurons developing interconnections. The cells are stained for neuronal tubulin (TUJI antibody, cyan) and DNA (DAPI, blue). Cultures were prepared by dissecting cortices from mouse embryos (E18), then mechanically dissociating single cells for plating. Explanted primary neurons placed in culture regress several stages back,

Featured image with Krystyna Gieniec

Posted by FocalPlane, on 1 August 2025

Our featured image, 'Unexpected guests' acquired by Krystyna Gieniec, is a 2D culture of mouse mammary fibroblasts stained for Acta2 (magenta) and Vimentin (gold), with some contaminating epithelial cells stained for pan-Cytokeratin (cyan). The image was acquired using a Leica Stellaris 8 confocal microscope.

Featured image with Maik Bischoff

Posted by FocalPlane, on 18 July 2025

Our featured image, acquired by Maik Bischoff, captures a portion of the testis from the fruit fly Drosophila hydei. Like all insect testes, it is enveloped by a muscle layer. F-actin was stained using phalloidin, making the muscle fibers visible, while DAPI staining revealed the multiple nuclei within each syncytial myotube. An antibody against Drosophila

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Microscopy-related articles from our journals

Toggle-Untoggle – a cell segmentation tool with an interactive user verification interface J Cell Sci 2025 138: jcs264154
The ratio of Wnt signaling activity to Sox2 transcription factor levels predicts neuromesodermal fate potential Development 2025 152: dev204661
The short isoform of Tango1 is dispensable for zebrafish survival but is required for skeletal patterning and integrity Biology Open 2025 14: bio062117

Microscopy-related preprint highlights

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Microscopy-related articles from our journals

Microscopy-related preprint highlights

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