Probes 101 – How to choose a fluorescent probe for your imaging experiment

Posted by Lina El Hajji, on 14 December 2024

Fluorescence microscopy is a powerful tool for the visualization of the intricate biological processes occurring within biological organisms. Just like you can make important pieces of information pop up in their notes using colorful highlighters, biologists have at hand a large toolkit of “highlighters” to make their favorite targets stand out in the complex cellular

Considerations for expression of fluorescent proteins and imaging in mammalian cells

Posted by Daniel Cabezas de la Fuente, on 7 July 2021

Introduction to fluorescent proteins  Fluorescent proteins have the property of absorbing light at one wavelength and emit light in a longer wavelength. These proteins were observed first in bioluminescent organisms known to humanity for centuries. We can find examples of light-emitting organisms in multiple taxa: from single cell organisms like bacteria, to vertebrates like fish.