Elisabeth Kugler

Elisabeth Kugler is a PostDoc at UCL, developing a quantitative model of retina neurovascular unit formation. She conducted her PhD at the University of Sheffield (UK) developing image segmentation, registration, and quantification pipelines for the zebrafish cerebral vasculature. Additionally, she discovered and characterised a previously undescribed cerebral endothelial cell (EC) membrane behaviour which she termed kugeln. Elisabeth combines experimental sciences with in silico modelling to understand the biological processes that underpin vascular development and disease.

#GliaMorph - a tool to analyse glial cell morphology in 3D

Posted by Elisabeth Kugler, on 17 May 2022

Contents What is #GliaMorph? What can you do and analyse with #GliaMorph? Links to manuscripts and data Contributions and Feedback Thanks to all contributors and people providing feedback References What is GliaMorph? GliaMorph is a toolkit implemented as Fiji Macros to allow modular application to process and analyse Müller glia morphology in the retina. During

LSFM series – Part IV: Data post-processing: Cropping, tailoring, and trimming

Posted by Elisabeth Kugler, on 23 January 2021

Elisabeth Kugler 1 and Emmanuel G. Reynaud 2 Contact:kugler.elisabeth@gmail.com; emmanuel.reynaud@ucd.ie There is a very thin sheet of light between gathering data and hoarding. In Science, between pilling up manuscripts on desk, books on shelves and samples in cold freezer, most of us fit in the latter category. It is OK, if you have space available

LSFM series – Surfing on the data freak wave! Part II: Before imaging: Know your sample (geometry)

Posted by Elisabeth Kugler, on 10 October 2020

This post, part of the blog series "LSFM series – Surfing on the data freak wave!", discusses (a) Sample preparation, (b) Light interaction with matter, (c) sample alignment and (d) checking fluorescence and calibration