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Elisabeth Kugler

Elisabeth Kugler is a PostDoc at UCL, developing a quantitative model of retina neurovascular unit formation. She conducted her PhD at the University of Sheffield (UK) developing image segmentation, registration, and quantification pipelines for the zebrafish cerebral vasculature. Additionally, she discovered and characterised a previously undescribed cerebral endothelial cell (EC) membrane behaviour which she termed kugeln. Elisabeth combines experimental sciences with in silico modelling to understand the biological processes that underpin vascular development and disease.

About Elisabeth Kugler

Scientific field: Developmental biology, Computational biology

Microscopy background: Image Analysis

Posts by Elisabeth Kugler

Part V The future: The hope of smart microscopes and phantoms

Posted by , on 24 April 2021

Elisabeth Kugler 1 and Emmanuel G. Reynaud 2 Contact: kugler.elisabeth@gmail.com; emmanuel.reynaud@ucd.ie We are reaching the end of our LSFM journey. Now it is time to look into the future, riding the plane of light! So, what is next for the LSFM? 1. Four-to-two, then four, then one. The story of SPIM, and LSFM per se,

LSFM series – Part IV: Data post-processing: Cropping, tailoring, and trimming

Posted by , on 23 January 2021

Elisabeth Kugler 1 and Emmanuel G. Reynaud 2 Contact:kugler.elisabeth@gmail.com; emmanuel.reynaud@ucd.ie There is a very thin sheet of light between gathering data and hoarding. In Science, between pilling up manuscripts on desk, books on shelves and samples in cold freezer, most of us fit in the latter category. It is OK, if you have space available

LSFM series – Part III: Image acquisition: Calibration and acquisition

Posted by , on 17 December 2020

Elisabeth Kugler 1 and Emmanuel G. Reynaud 2 Contact:kugler.elisabeth@gmail.com; emmanuel.reynaud@ucd.ie Now, we are getting closer to the point of setting up our sample in the chamber to image it. Now that we know which microscope we are about to use (Part I) and have mounted it the right way (Part II), we need to trust

LSFM series – Surfing on the data freak wave! Part II: Before imaging: Know your sample (geometry)

Posted by , on 10 October 2020

This post, part of the blog series "LSFM series – Surfing on the data freak wave!", discusses (a) Sample preparation, (b) Light interaction with matter, (c) sample alignment and (d) checking fluorescence and calibration

LSFM series – Surfing on the data freak wave! PART I: Knowing your turf, knowing your surf!

Posted by , on 5 September 2020

Here we present a series of five blog posts with tips and tricks about light sheet microscopy. 1. The basics of LSFM (Sept 2020) 2. Improving sample mounting (Oct 2020) 3. Calibration and Acquisition (Nov 2020) 4. Tailoring the data (Dec 2020) 5. What is next? AI and smarter than us LSFM (Jan 2021)

Recent gallery items by Elisabeth Kugler