Elisabeth Kugler is a PostDoc at UCL, developing a quantitative model of retina neurovascular unit formation. She conducted her PhD at the University of Sheffield (UK) developing image segmentation, registration, and quantification pipelines for the zebrafish cerebral vasculature. Additionally, she discovered and characterised a previously undescribed cerebral endothelial cell (EC) membrane behaviour which she termed kugeln. Elisabeth combines experimental sciences with in silico modelling to understand the biological processes that underpin vascular development and disease.
Scientific field: Developmental biology, Computational biology
Microscopy background: Image Analysis
Posted by Elisabeth Kugler, on 24 April 2021Elisabeth Kugler 1 and Emmanuel G. Reynaud 2 Contact: email@example.com; firstname.lastname@example.org We are reaching the end of our LSFM journey. Now it is time to look into the future, riding the plane of light! So, what is next for the LSFM? 1. Four-to-two, then four, then one. The story of SPIM, and LSFM per se,
Posted by Elisabeth Kugler, on 23 January 2021Elisabeth Kugler 1 and Emmanuel G. Reynaud 2 Contact:email@example.com; firstname.lastname@example.org There is a very thin sheet of light between gathering data and hoarding. In Science, between pilling up manuscripts on desk, books on shelves and samples in cold freezer, most of us fit in the latter category. It is OK, if you have space available
Posted by Elisabeth Kugler, on 17 December 2020Elisabeth Kugler 1 and Emmanuel G. Reynaud 2 Contact:email@example.com; firstname.lastname@example.org Now, we are getting closer to the point of setting up our sample in the chamber to image it. Now that we know which microscope we are about to use (Part I) and have mounted it the right way (Part II), we need to trust
Posted by Elisabeth Kugler, on 10 October 2020This post, part of the blog series "LSFM series – Surfing on the data freak wave!", discusses (a) Sample preparation, (b) Light interaction with matter, (c) sample alignment and (d) checking fluorescence and calibration
Posted by Elisabeth Kugler, on 5 September 2020Here we present a series of five blog posts with tips and tricks about light sheet microscopy. 1. The basics of LSFM (Sept 2020) 2. Improving sample mounting (Oct 2020) 3. Calibration and Acquisition (Nov 2020) 4. Tailoring the data (Dec 2020) 5. What is next? AI and smarter than us LSFM (Jan 2021)