Gallery

Featured image

Our featured image, acquired by Subhajit Karmakar, depicts a dividing MDA-MB-231 cancer cell that has been stained to visualise the localisation of two key proteins: CK2, a highly oncogenic kinase, and an E3 ligase, a tumour suppressor. The study focusses on the variation in CK2 localisation under serum-starved conditions, aiming to understand its cellular distribution in response to nutrient deprivation. To prepare the sample, the cells were fixed with 4% paraformaldehyde to preserve their structure. They were then incubated with primary antibodies specific to CK2 and the E3 ligase, followed by secondary antibodies conjugated to Alexa Fluor dyes for fluorescence detection. Finally, the cells were mounted with DAPI to stain the nuclei, allowing for clear visualisation of the cell’s overall structure and the specific localisation of the proteins. This approach provides valuable insights into the dynamic behaviour of these proteins in response to cellular stress.

The copyright for all the images in our gallery remains with the author unless otherwise stated. Please contact the author if you would like to use an image for any purpose.

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