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Illuminating the Brain: Microscopy Techniques in Neuroscience and My Applications in the Lab

Posted by Sally Horton, on 23 June 2025

Understanding the brain’s complexity requires more than theory or behaviour; it demands a look at the smallest units of structure and function. Microscopy has long been a cornerstone of neuroscience, evolving rapidly from conventional optical methods to cutting-edge super-resolution and electron techniques. This article explores how confocal microscopy, STORM, and electron microscopy are advancing our

Workshop report: France BioImaging - Advanced Training on Light-Sheet Microscopy

Posted by Clara Hayn, on 22 May 2025

From November 4th to 7th, 2024, I had the privilege to attend the Advanced Training on Light-Sheet Microscopy‘s Principles and Applications to Neuroscience and 3D Cell Culture at France BioImaging in Bordeaux. This comprehensive training, rich in both theoretical and practical knowledge, provided an exceptional platform for learning and engaging with experts in the field.

Advantages of LED Illumination for Neuroscience Research

Sponsored by CoolLED, on 26 September 2024

LED microscopy illumination has disrupted traditional approaches to high-speed imaging and optogenetic stimulation for neuroscience, and the CoolLED Support Team are available to help researchers understand which filters, light sources and other components are right for each individual setup.

An interview with Maribel Torres Velázquez

Posted by Mariana De Niz, on 6 August 2024

MiniBio: Maribel Torres Velázquez is a NURTURE postdoctoral fellow in the lab of Prof. Gregory Schwartz at the Feinberg School of Medicine at Northwestern University, where she uses microscopy to study the retina-to-brain circuitry. Specifically, she aims to uncover the retina-brain connectivity map by identifying to which unique brain areas different retinal ganglion cell sub-types

Microscopy preprints – applications in biology

Posted by FocalPlane, on 19 April 2024

In this post, we share preprints that use microscopy tools to answer questions in biology.

Featured image with Thibault Dhellemmes and Jérémie Teillon

Posted by FocalPlane, on 1 March 2024

Our featured image shows the full morphology of Relaxin-3 neurons in an entire adult mouse brain. To observe the full depth of this brain, it was made transparent by applying an optical clearing technique called Adipoclear. The sample was observed on an Ultramicroscope II light sheet microscope and is a maximum intensity projection over the

Enhancing Global Access: interview with CZI grantee Michelle Itano

Posted by Mariana De Niz, on 24 January 2024

ENHANCING THE ROLE OF A MICROSCOPY CORE FACILITY TO CATALYSE BIOMEDICAL RESEARCH Michelle Itano is a cellular biophysicist and Director of the University of North Carolina Chapel Hill Neuroscience Microscopy Core. Her project for the Cycle 1 Imaging Scientists CZI funding call aims to actively engage and grow collaboration between researchers and computer scientists to

Featured image with Nick Gatford

Posted by FocalPlane, on 16 November 2023

Our featured image shows a single human dopaminergic neuron generated from a human stem cell, acquired via super-resolution Airyscan confocal microscopy at the University of Oxford Micron facility by Nick Gatford. We caught up with Nick to find out about his research and what he is excited about in microscopy.

The FocalPlane correspondents: Marta Forcella

Posted by FocalPlane, on 26 July 2023

At the beginning of the year, we announced that we will be working with three FocalPlane correspondents, who will be helping us to develop and write content for FocalPlane. We caught up with each of them to chat about their research backgrounds and the topics that they’re excited to write about. Here, we talk to

An interview with Brenda Delfin

Posted by Mariana De Niz, on 6 June 2023

MiniBio: Brenda Delfin is a researcher at Universidad Peruana Cayetano Heredia, in Lima, Peru. She is a Biologist by training, and has specialized in Neuroscience. She is currently head of the animal house and interim leader of the Neuroscience of development lab at UPCH. Joining her expertise in neuroscience and her passion for teaching, learning

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Microscopy-related articles from our journals

HCR spectral imaging: 10-plex, quantitative, high-resolution RNA and protein imaging in highly autofluorescent samples Development 2024 151: dev202307
filoVision – using deep learning and tip markers to automate filopodia analysis J Cell Sci 2024 137: jcs261274
C omputational tools for quantifying actin filament numbers, lengths, and bundling Biology Open 2024 13: bio.060267

Microscopy-related preprint highlights

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Microscopy-related articles from our journals

Microscopy-related preprint highlights

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