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Posted by , on 8 June 2021

Today we start a new monthly post. We have asked three researchers to propose their three favourite recently published papers (related to microscopy, of course).

You might be up to date with the recent literature in your field of research, but what about other disciplines? We hope this list, provided this month by Claudia Almeida, Joachim Goedhart and Stephen Royle, helps you find some interesting publications that were not included in your reading list.

These are the suggestions for this month:

Claudia G. Almeida

Laboratory of neuronal trafficking in aging

CEDOC – Universidade Nova de Lisboa

Image extracted from Skjeldal et al., 2021

Joachim Goedhart

Swammerdam Institute for Life Sciences – University of Amsterdam

  • ‘The HaloTag as a general scaffold for far-red tunable chemigenetic indicators’ https://www.nature.com/articles/s41589-021-00775-w Hybrid fluorescent probes that combine a genetically encoded tag with an exogenously applied fluorophore have made substantial progress over recent years. This paper presents another leap forward, generating hybrid biosensors with flexible fluorophore choice to visualize calcium changes in cells.
  • ‘Dynamic FRET-FLIM based screens of signal transduction pathways: a feasibility study’ https://doi.org/10.1101/2021.05.23.445328 Lifetime-based methods have several advantages over intensity based microscopy methods, but is lifetime based screening with FRET sensors feasible? This study presents a quantitative platform for screening of cAMP dynamics in single cells.
Image extracted from Harkes et al., 2021

Stephen J. Royle

Centre for Mechanochemical Cell Biology
Warwick Medical School – University of Warwick

  • ‘Morpho-functional characterization of the endo-lysosomal system by high-throughput correlative light-electron microscopy’ https://www.biorxiv.org/content/10.1101/2021.05.21.445146v1 This paper demonstrates that CLEM can be used in place of immunoEM to identify distinct stages of the endo-lysosomal system by the presence of well-established molecular marker proteins.
Image extracted from van der Beek et al., 2021
  • ‘STED super-resolution imaging of membrane packing and dynamics by exchangeable polarity-sensitive dyes’ https://www.biorxiv.org/content/10.1101/2021.06.05.446432v1 A new membrane dye, which is remarkably resistant to photobleaching, is developed (based on Nile Red) and enables high resolution 3D-STED imaging of plasma membrane events.






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