Microscopy preprints – new tools and techniques

Posted by FocalPlane, on 6 May 2022

Here is a curated selection of preprints published recently. In this post, we focus specifically on new imaging tools only.

Content-aware image restoration improves spatiotemporal resolution in luminescence microscopy
Tobias Boothe, Mario Ivanković, Markus A. Grohme, Jochen C. Rink

Focus-ISM for Sharp and Gentle Super-Resolved Microscopy
Giorgio Tortarolo, Alessandro Zunino, Francesco Fersini, Marco Castello, Simonluca Piazza, Colin J.R. Sheppard, Paolo Bianchini, Alberto Diaspro, Sami Koho, Giuseppe Vicidomini

Figure extracted from Tortarolo *et al.*

Improved imaging and preservation of lysosome dynamics using silver nanoparticle-enhanced fluorescence
Sumaiya A. Soha, Araniy Santhireswaran, Gregory K. Hodgson, Michael Sugiyama, Costin N. Antonescu, Stefania Impellizzeri, Roberto J. Botelho

Integrated optical device for Structured Illumination Microscopy
Matteo Calvarese, Petra Paiè, Alessia Candeo, Gianmaria Calisesi, Francesco Ceccarelli, Gianluca Valentini, Roberto Osellame, Hai Gong, Mark A. Neil, Francesca Bragheri, Andrea Bassi

High-fidelity 3D live-cell nanoscopy through data-driven enhanced super-resolution radial fluctuation
Romain F. Laine, Hannah S. Heil, Simao Coelho, Jonathon Nixon-Abell, Angélique Jimenez, Tommaso Galgani, Aki Stubb, Gautier Follain, Siân Culley, Guillaume Jacquemet, Bassam Hajj, Christophe Leterrier, Ricardo Henriques

An Integrated Platform for High-Throughput Nanoscopy
Andrew E S Barentine, Yu Lin, Edward M Courvan, Phylicia Kidd, Miao Liu, Leonhard Balduf, Timy Phan, Felix Rivera-Molina, Michael R Grace, Zach Marin, Mark Lessard, Juliana Rios Chen, Siyuan Wang, Karla M Neugebauer, Joerg Bewersdorf, David Baddeley

Scanning Single Molecule Localization Microscopy (scanSMLM) for super-resolution optical volume imaging
Jigmi Basumatary, Neptune Baro, Prakash Joshi, Partha Pratim Mondal

Figure extracted from Basumatary *et al.*

resPAINT: Accelerating volumetric super-resolution localisation microscopy by active control of probe emission
Edward W. Sanders, Alexander R. Carr, Ezra Bruggeman, Markus Koerbel, Sarah I. Benaissa, Robert F. Donat, Ana Mafalda Santos, James McColl, Kevin O’Holleran, David Klenerman, Simon J. Davis, Steven F. Lee, Aleks Ponjavic

Stimulated emission depletion microscopy with a single depletion laser using five fluorochromes and fluorescence lifetime phasor separation
Mariano Gonzalez Pisfil, Iliya Nadelson, Brigitte Bergner, Sonja Rottmeier, Andreas W. Thomae, Steffen Dietzel

Figure extracted from Gonzalez Pisfil *et al.*

LILAC: Enhanced actin imaging with an optogenetic Lifeact
Kourtney L. Kroll, Alexander R. French, Tobin R. Sosnick, Ronald S. Rock

All-optical visualization of specific molecules in the ultrastructural context of brain tissue
Ons M’Saad, Ravikiran Kasula, Ilona Kondratiuk, Phylicia Kidd, Hanieh Falahati, Juliana E. Gentile, Robert F. Niescier, Katherine Watters, Robert C. Sterner, Seong Lee, Xinran Liu, Pietro De Camilli, James E. Rothman, Anthony J. Koleske, Thomas Biederer, Joerg Bewersdorf