Microscopy preprints – new tool and techniques in imaging

Here is a curated selection of preprints posted recently on new tools and techniques in imaging, including a tool from Lukas Kapitein and colleagues for accurately measuring expansion for ExM and a comparison between spectral separation and demixing for fast multicolour SMLM from Christophe Leterrier and colleagues.

Self-pressurized rapid freezing at arbitrary cryoprotectant concentrations
K. Rolle, K.A. Okotrub, I.V. Zaytseva, S.A. Babin, N.V. Surovtsev

Correlation Single Molecule Localization Microscopy (corrSMLM) Detects Fortunate Molecules for High Signal-to-Background Ratio and Better Localization Precision
S Aravinth, Francesca C. Zanacchi, Partha P. Mondal

The K2: Open-source simultaneous triple-color TIRF microscope for live-cell and single-molecule imaging
Christian Niederauer, Marco Seynen, Jan Zomerdijk, Marko Kamp, Kristina A. Ganzinger

GelMap: Intrinsic calibration and deformation mapping for expansion microscopy
Hugo G.J. Damstra, Josiah B. Passmore, Albert K. Serweta, Ioannis Koutlas, Mithila Burute, Frank J. Meye, Anna Akhmanova, Lukas C. Kapitein

Genetically encoded multimeric tags for intracellular protein localisation in cryo-EM
Herman KH Fung, Yuki Hayashi, Veijo T Salo, Anastasiia Babenko, Ievgeniia Zagoriy, Andreas Brunner, Jan Ellenberg, Christoph W Müller, Sara Cuylen-Haering, Julia Mahamid

Enhancing detectable fluorescence fluctuation for high-throughput and four-dimensional live-cell super-resolution imaging
Weisong Zhao, Shiqun Zhao, Zhenqian Han, Xiangyan Ding, Guangwei Hu, Xinwei Wang, Heng Mao, Yaming Jiu, Ying Hu, Jiubin Tan, Xumin Ding, Changliang Guo, Liangyi Chen, Haoyu Li

Dual-color single molecule localization microscopy on transparent polymer waveguide chips
Anders Kokkvoll Engdahl, Surjendu Bikash Dutta, Stefan Belle, Jasmin Schürstedt, Karolina Szafranska, Peter McCourt, Ralf Hellmann, Thomas Huser, Mark Schüttpelz

Spatial frequency-based correction of the spherical aberration in living brain imaging
Aoi Gohma, Naoya Adachi, Daiki Horiba, Yasuo Yonemaru, Daisuke Nishiwaki, Eiji Yokoi, Kaori Higuchi, Yoshihiro Ue, Atsushi Miyawaki, Hiromu Monai

Bleaching-resistant, near-continuous single-molecule fluorescence and FRET based on fluorogenic and transient DNA binding
Mirjam Kümmerlin, Abhishek Mazumder, Achillefs N. Kapanidis

Selective volumetric excitation and imaging for single molecule localization microscopy in multicellular systems
Tommaso Galgani, Yasmina Fedala, Romeo Zapata, Laura Caccianini, Virgile Viasnoff, Jean-Baptiste Sibarita, Rémi Galland, Maxime Dahan, Bassam Hajj

A Modular Approach to Active Focus Stabilization for Fluorescence Microscopy
Birthe van den Berg, Robin Van den Eynde, Baptiste Amouroux, Marcel Müller, Peter Dedecker, Wim Vandenberg

Femtosecond laser preparation of resin embedded samples for correlative microscopy workflows in life sciences
C Bosch, J Lindenau, A Pacureanu, CJ Peddie, M Majkut, AC Douglas, R Carzaniga, A Rack, L Collinson, AT Schaefer, H Stegmann

Label-free and non-destructive pathology of human lung adenocarcinomas with ultraviolet single-plane illumination microscopy
Yan Zhang, Bingxin Huang, Lei Kang, Victor T. C. Tsang, Jiajie Wu, Claudia T. K. Lo, Terence T. W. Wong

Enhanced neuroimaging with a calcium sensor in the live adult Drosophila Melanogaster brain using closed-loop adaptive optics light-sheet microscopy
Antoine Hubert, Georges Farkouh, Fabrice Harms, Cynthia Veilly, Sophia Imperato, Mathias Mercier, Vincent Loriette, François Rouyer, Alexandra Fragola

Label-free high-resolution infrared spectroscopy for spatiotemporal analysis of complex living systems
Nika Gvazava, Sabine Konings, Efrain Cepeda-Prado, Valeriia Skoryk, Chimezie H. Umeano, Jiao Dong, Iran A.N. Silva, Daniella Rylander Ottosson, Nicholas D. Leigh, Darcy E. Wagner, Oxana Klementieva

Oxygen-induced chromophore degradation in the photoswitchable red fluorescent protein rsCherry
Thi Yen Hang Bui, Elke De Zitter, Benjamien Moeyaert, Ludovic Pecqueur, Bindu Y Srinivasu, Anastassios Economou, Marc Fontecave, Luc Van Meervelt, Peter Dedecker, Brandán Pedre

Enhanced neuroimaging with a calcium sensor in the live adult Drosophila Melanogaster brain using closed-loop adaptive optics light-sheet microscopy
Antoine Hubert, Georges Farkouh, Fabrice Harms, Cynthia Veilly, Sophia Imperato, Mathias Mercier, Vincent Loriette, François Rouyer, Alexandra Fragola

Technical insights into fluorescence lifetime microscopy of mechanosensitive Flipper probes
Chloe Roffay, Juan Manuel Garcia Arcos, Pierrik Chapuis, Javier Lopez-Andarias, Falk Schneider, Adai Colom, Caterina Tomba, Ilaria Di Meglio, Valentin Dunsing, Stefan Matile, Aurelien Roux, Vincent Mercier

Extracting nanoscale morphology from localizations
Zach Marin, Lukas A. Fuentes, Joerg Bewersdorf, David Baddeley

Robust and fast multicolor Single Molecule Localization Microscopy using spectral separation and demixing
Karoline Friedl, Adrien Mau, Valentina Caorsi, Nicolas Bourg, Sandrine Lévêque-Fort, Christophe Leterrier

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