Microscopy preprints – new tools and techniques in imaging

Posted by FocalPlane, on 20 October 2023

Here is a curated selection of preprints posted recently on new tools and techniques in imaging. Let us know if we are missing any recent preprints that are on your reading list!

Deep learning-based aberration compensation improves contrast and resolution in fluorescence microscopy
Min Guo, Yicong Wu, Yijun Su, Shuhao Qian, Eric Krueger, Ryan Christensen, Gran Kroeschell, Johnny Bui, Matthew Chaw, Lixia Zhang, Jiamin Liu, Xuekai Hou, Xiaofei Han, Xuefei Ma, Alexander Zhovmer, Christian Combs, Mark Moyle, Eviatar Yemini, Huafeng Liu, Zhiyi Liu, Patrick La Riviere, Daniel Colón-Ramos, Hari Shroff

Figure extracted from Guo, *et al.* made available under a CC-BY 4.0 International license.

Axial de-scanning using remote focusing in the detection arm of light-sheet microscopy
Hassan Dibaji, Ali Kazemi Nasaban Shotorban, Mahsa Habibi, Rachel M Grattan, Shayna Lucero, David J Schodt, Keith A Lidke, Jonathan Petruccelli, Diane S Lidke, Sheng Liu, Tonmoy Chakraborty

Optimal Sampling Rate for 3D Single Molecule Localization
Huanzhi Chang, Shuang Fu, Yiming Li

Quantum-assisted Adaptive Optics for Microscopy
Patrick Cameron, Baptiste Courme, Daniele Faccio, Hugo Defienne

Figure extracted from Cameron, *et al.* made available under a CC-BY 4.0 International license.

Single-shot experimental-numerical twin-image removal in lensless digital holographic microscopy
Piotr Arcab, Mikolaj Rogalski, Maciej Trusiak

Figure extracted from Arcab, *et al.* made available under a CC-BY 4.0 International license.

Motion Blur Microscopy
Utku Goreke, Ayesha Gonzales, Brandon Shipley, Yuncheng Man, William Wulftange, Ran An, Michael Hinczewski, Umut A. Gurkan

Figure extracted from Goreke, *et al.* made available under a CC-BY-NC 4.0 International license.

Multi-scale tissue fluorescence mapping with fibre optic ultraviolet excitation and generative modelling
Joel Lang Yi Ang, Ko Hui Tan, Alexander Si Kai Yong, Chiyo Wan Xuan Tan, Jessica Sze Jia Kng, Cyrus Jia Jun Tan, Rachael Hui Kie Soh, Julian Yi Hong Tan, Kaicheng Liang

Whole-cell multi-target single-molecule super-resolution imaging in 3D with microfluidics and a single-objective tilted light sheet
Nahima Saliba, Gabriella Gagliano, Anna-Karin Gustavsson

Open top multi sample dual view light sheet microscope for live imaging of large multicellular systems
F. Moos, S. Suppinger, G. de Medeiros, K. Oost, A. Boni, C. Rémy, S. Weevers, C. Tsiairis, P. Strnad, P. Liberali

Label-free 3-D molecular imaging of living tissues using Raman Spectral Projection Tomography
Elzbieta Stepula, Anders R. Walther, Dev Mehrotra, Magnus Jensen, Mu H. Yuan, Simon V. Pedersen, Eileen Gentleman, Michael B. Albro, Martin A. B. Hedegaard, Mads S. Bergholt

A Low-Cost Stage-Top Incubation Device For Human Cell Imaging Using Rapid Prototyping Methods
Michael Worcester, Melissa Gomez, Pratyasha Mishra, Quintin Meyers, Thomas E. Kuhlman

XLuminA: An Auto-differentiating Discovery Framework for Super-Resolution Microscopy
Carla Rodríguez, Sören Arlt, Leonhard Möckl, Mario Krenn

Dipole-Spread Function Engineering for 6D Super-Resolution Microscopy
Tingting Wu, Matthew D. Lew

Millimeter-deep micron-resolution vibrational imaging by shortwave infrared photothermal microscopy
Hongli Ni, Yuhao Yuan, Mingsheng Li, Yifan Zhu, Xiaowei Ge, Chinmayee Prabhu Dessai, Le Wang, Ji-Xin Cheng

Smartphone-based Optical Sectioning (SOS) Microscopy with A Telecentric Design for Fluorescence Imaging
Ziao Jiao, Mingliang Pan, Khadija Yousaf, Daniel Doveiko, Michelle Maclean, David Griffin, Yu Chen, David Day Uei Lia

Figure extracted from Jiao, *et al.* made available under a CC-BY 4.0 International license.

EventLFM: Event Camera integrated Fourier Light Field Microscopy for Ultrafast 3D imaging
Ruipeng Guo, Qianwan Yang, Andrew S. Chang, Guorong Hu, Joseph Greene, Christopher V. Gabel, Sixian You, Lei Tian

Spatial wavefront shaping with a multipolar-resonant metasurface for structured illumination microscopy
Tamal Roy, Peter T. Brown, Douglas P. Shepherd, Lisa V. Poulikakos

Multi-Spectral Reflection Matrix for Ultra-Fast 3D Label-Free Microscopy
Paul Balondrade, Victor Barolle, Nicolas Guigui, Emeric Auriant, Nathan Rougier, Claude Boccara, Mathias Fink, Alexandre Aubry

Laboratory-Based Correlative Soft X-ray and Fluorescence Microscopy in an Integrated Setup
Julius Reinhard, Sophia Kaleta, Johann Jakob Abel, Felix Wiesner, Martin Wünsche, Eric Seemann, Martin Westermann, Thomas Weber, Jan Nathanael, Alexander Iliou, Henryk Fiedorowicz, Falk Hillmann, Christian Eggeling, Gerhard G. Paulus, Silvio Fuchs

Figure extracted from Reinhard, *et al.* made available under a CC-BY 4.0 International license.

Comparison of polystyrene and hydrogel microcarriers for optical imaging of adherent cells
Oscar R. Benavides, Berkley P. White, Holly C. Gibbs, Roland Kaunas, Carl A. Gregory, Kristen C. Maitland, Alex J. Walsh

Per-pixel unmixing of spectrally overlapping fluorophores using intra-exposure excitation modulation
Hana Valenta, Franziska Bierbuesse, Raffaele Vitale, Cyril Ruckebusch, Wim Vandenberg, Peter Dedecker

Expansion Mass Spectrometry Imaging: Achieving One-Micron Level Resolution for Lipid Analysis in Tissues
Yik Ling Winnie Hung, Chengyi Xie, Jianing Wang, Xin Diao, Xiaoxiao Wang, Shulan Qiu, Jiacheng Fang, Zongwei Cai

Sample preparation and data collection for Serial Block Face Scanning Electron Microscopy of Mammalian Cell Monolayers
Noelle V. Antao, Joseph Sall, Christopher Petzold, Damian C. Ekiert, Gira Bhabha, Feng-Xia Liang

Unveiling the ultrastructural landscape of native extracellular matrix via lift-out cryo-FIBSEM and cryo-ET
Bettina Zens, Florian Fäßler, Jesse M Hansen, Robert Hauschild, Julia Datler, Victor-Valentin Hodirnau, Vanessa Zheden, Jonna Alanko, Michael Sixt, Florian KM Schur

Advancement in Cellular Topographic and Nanoparticle Capture Imaging by High Resolution Microscopy Incorporating a Freeze-Drying and Gaseous Nitrogen-based Approach
Kunihiro Uryu, Nadine Soplop, Timothy P. Sheahan, Maria-Teresa Catanese, Chuong Huynh, John Pena, Nancy Boudreau, Irina Matei, Candia Kenific, Ayako Hashimoto, Ayuko Hoshino, Charles M. Rice, David Lyden

An optogenetic method for the controlled release of single molecules
Purba Kashyap, Sara Bertelli, Fakun Cao, Yulia Kostritskaia, Fenja Blank, Niranjan Srikanth, Roberto Saleppico, Dolf Bierhuizen, Xiaocen Lu, Walter Nickel, Robert E. Campbell, Andrew Plested, Tobias Stauber, Marcus J. Taylor, Helge Ewers

CaBLAM! A high-contrast bioluminescent Ca2+ indicator derived from an engineered Oplophorus gracilirostris luciferase
Gerard G. Lambert, Emmanuel L. Crespo, Jeremy Murphy, Daniela Boassa, Selena Luong, Dmitrijs Celinskis, Stephanie Venn, Junru Hu, Brittany Sprecher, Maya O. Tree, Richard Orcutt, Daniel Heydari, Aidan B. Bell, Albertina Torreblanca-Zanca, Ali Hakimi, Diane Lipscombe, Christopher I. Moore, Ute Hochgeschwender, Nathan C. Shaner

A General Method to Accurately Count Molecular Complexes and Determine the Degree of Labelling in Cells Using Protein Tags
Stanimir Asenov Tashev, Jonas Euchner, Klaus Yserentant, Siegfried Hänselmann, Felix Hild, Wioleta Chmielewicz, Johan Hummert, Florian Schwörer, Nikolaos Tsopoulidis, Stefan Germer, Zoe Saßmannshausen, Oliver T. Fackler, Ursula Klingmüller, Dirk-Peter Herten

Electrochemically controlled blinking of fluorophores to enable quantitative stochastic optical reconstruction microscopy (STORM) imaging
Ying Yang, Yuanqing Ma, Richard Tilley, Katharina Gaus, J. Justin Gooding

Recombinant biosensors for multiplex and super-resolution imaging of phosphoinositides
Hannes Maib, Petia Adarska, Robert Hunton, James Vines, David Strutt, Francesca Bottanelli, David H Murray

Figure extracted from Maib, *et al.* made available under a CC-BY-NC 4.0 International license.

Contact-FP: a dimerization-dependent fluorescent protein toolkit for visualizing membrane contact site dynamics
Gregory E. Miner, Sidney Y. Smith, Wendy K. Showalter, Christina M. So, Joey V. Ragusa, Alex E. Powers, Maria Clara Zanellati, Chih-Hsuan Hsu, Michelle F. Marchan, Sarah Cohen

Fast, whole-cell DNA-PAINT imaging with dual-labeled self-quenched imager probes
Arindam Ghosh, Mara Meub, Dominic A. Helmerich, Sören Doose, Markus Sauer

A red-emitting carborhodamine for monitoring and measuring membrane potential
Anneliese M. M. Gest, Julia R. Lazzari-Dean, Gloria Ortiz, Susanna K. Yaeger-Weiss, Steven C. Boggess, Evan W. Miller