Microscopy preprints – new tools and techniques in imaging

Posted by FocalPlane, on 1 December 2023

Here is a curated selection of preprints posted recently on new tools and techniques in imaging. Let us know if we are missing any recent preprints that are on your reading list!

Simultaneous multimodal three-photon and optical coherence microscopy of the mouse brain in the 1700 nm optical window in vivo
Xusan Yang, Siyang Liu, Fei Xia, Meiqi Wu, Steven Adie, Chris Xu

SmartEM: machine-learning guided electron microscopy
Yaron Meirovitch, Core Francisco Park, Lu Mi, Pavel Potocek, Shashata Sawmya, Yicong Li, Yuelong Wu, Richard Schalek, Hanspeter Pfister, Remco Schoenmakers, Maurice Peemen, Jeff W. Lichtman, Aravinthan D.T. Samuel, Nir Shavit

Figure extracted from Meirovitch, *et al.* made available under a CC-BY-NC 4.0 International license.

Sample preparation and data collection for Serial Block Face Scanning Electron Microscopy of Mammalian Cell Monolayers
Noelle V. Antao, Joseph Sall, Christopher Petzold, Damian C. Ekiert, Gira Bhabha, Feng-Xia Liang

Impact of a tilted coverslip on two-photon and STED microscopy
Guillaume Le Bourdelles, Luc Mercier, Johannes Roos, Stephane Bancelin, U. Valentin Nägerl

A fluorogenic complementation tool kit for interrogating lipid droplet-organelle interaction
Xiao Li, Rico Gamuyao, Ming-Lun Wu, Woo Jung Cho, Nathan B Kurtz, Sharon V King, R A Petersen, Daniel R Stabley, Caleb Lindow, Leslie Climer, Abbas Shirinifard, Francesca Ferrara, Robert E Throm, Camenzind G Robinson, Alex Carisey, Alison G Tebo, Chi-Lun Chang

Printing, Characterising, and Assessing Transparent 3D Printed Lenses for Optical Imaging
Liam M. Rooney, Jay Christopher, Ben Watson, Yash Susir Kumar, Laura Copeland, Lewis D. Walker, Shannan Foylan, William B. Amos, Ralf Bauer, Gail McConnell

Figure extracted from Rooney, *et al.* made available under a CC-BY 4.0 International license.

Quantifying fluorescence lifetime responsiveness of environment sensitive probes for membrane fluidity measurements
Franziska Ragaller, Ellen Sjule, Yagmur Balim Urem, Jan Schlegel, Rojbin El, Dunja Urbancic, Iztok Urbancic, Hans Blom, Erdinc Sezgin

Figure extracted from Ragaller, *et al.* made available under a CC-BY-NC 4.0 International license.

StayGold Photostability under Different Illumination Modes
Masahiko Hirano, Yasuo Yonemaru, Satoshi Shimozono, Mayu Sugiyama, Ryoko Ando, Yasushi Okada, Takahiro Fujiwara, Atsushi Miyawaki

Figure extracted from Hirano, *et al.* made available under a CC-BY 4.0 International license..

Engineering luminopsins with improved coupling efficiencies
Ashley Slaviero, Nipun Gorantla, Jacob Simkins, Emmanuel L Crespo, Ebenezer C Ikefuama, Maya O Tree, Mansi Prakash, Andreas Björefeldt, Lauren M Barnett, Gerard G Lambert, Diane Lipscombe, Christopher I Moore, Nathan C Shaner, Ute Hochgeschwender

Fast and robust 3D MINFLUX excitation with a variable phase plate
Takahiro Deguchi, Jonas Ries

Figure extracted from Deguchi and Ries made available under a CC-BY 4.0 International license.

Automated 3D multi-color single-molecule localization microscopy
Rory M. Power, Aline Tschanz, Timo Zimmermann, Jonas Ries

Figure extracted from Power, *et al.* made available under a CC-BY 4.0 International license.

Full-aperture extended-depth oblique plane microscopy through dynamic remote focusing
Paolo Pozzi, Vipin Balan, Alessia Candeo, Alessia Brix, Anna Silvia Pistocchi, Cosimo D’Andrea, Gianluca Valentini, Andrea Bassi

Figure extracted from Pozzi, *et al.* made available under a CC-BY 4.0 International license.

Fast and light efficient remote focusing for volumetric voltage imaging
Urs L. Böhm, Benjamin Judkewitz

Raman microscopy of cryofixed biological specimens for high-resolution and high-sensitivity chemical imaging
Kenta Mizushima, Yasuaki Kumamoto, Shoko Tamura, Masahito Yamanaka, Kentaro Mochizuki, Menglu Li, Syusuke Egoshi, Kosuke Dodo, Yoshinori Harada, Nicholas I. Smith, Mikiko Sodeoka, Hideo Tanaka, Katsumasa Fujita

Proximity Labeling Expansion Microscopy (PL-ExM) resolves structure of the interactome
Sohyeon Park, Xiaorong Wang, Xiangpeng Li, Xiao Huang, Katie C. Fong, Clinton Yu, Arthur A. Tran, Lorenzo Scipioni, Zhipeng Dai, Lan Huang, Xiaoyu Shi

Hyperspectral Oblique Plane Microscopy Enables Spontaneous, Label-Free Imaging of Biological Dynamic Processes in Live Animals
Ke Guo, Konstantinos Kalyviotis, Periklis Pantazis, Christopher J Rowlands

Extended-depth of field random illumination microscopy, EDF-RIM, provides super-resolved projective imaging
Lorry Mazzella, Thomas Mangeat, Guillaume Giroussens, Benoit Rogez, Hao Li, Justine Creff, Mehdi Saadaoui, Carla Martins, Simon Labouesse, Jérome Idier, Frédéric Galland, Marc Allain, Anne Sentenac, Loïc LeGoff

GAUSS-EM: Guided accumulation of ultrathin serial sections with a static magnetic field for volume electron microscopy
Kara A Fulton, Paul V Watkins, Kevin L Briggman

An open-source, high resolution, automated fluorescence microscope
Ando C. Zehrer, Ana Martin-Villalba, Benedict Diederich, Helge Ewers

Universal inverse modelling of point spread functions for SMLM localization and microscope characterization
Sheng Liu, Jianwei Chen, Jonas Hellgoth, Lucas-Raphael Müller, Boris Ferdman, Christian Karras, Dafei Xiao, Keith A. Lidke, Rainer Heintzmann, Yoav Shechtman, Yiming Li, Jonas Ries

Figure extracted from Liu, *et al.* made available under a CC-BY 4.0 International license.

A Large Field-of-View Two- and Three-Photon Microscope for High-Resolution Deep Tissue Imaging
Aaron T. Mok, Tianyu Wang, Shitong Zhao, Kristine E. Kolkman, Danni Wu, Dimitre G Ouzounov, Changwoo Seo, Chunyan Wu, Joseph R. Fetcho, Chris Xu

Fast and universal single molecule localization using multi-dimensional point spread functions
Mengfan Li, Wei Shi, Sheng Liu, Shuang Fu, Yue Fei, Lulu Zhou, Yiming Li

Deep Learning-based Optical Aberration Estimation Enables Offline Digital Adaptive Optics and Super-resolution Imaging
Chang Qiao, Haoyu Chen, Run Wang, Tao Jiang, Yuwang Wang, Dong Li

Developing a Toolbox of Antibodies Validated for Array Tomography-Based Imaging of Brain Synapses
Kristina D. Micheva, Belvin Gong, Forrest Collman, Richard J. Weinberg, Stephen J. Smith, James S. Trimmer, Karl D. Murray

pHusion: A robust and versatile toolset for automated detection and analysis of exocytosis
Ellen C. O’Shaughnessy, Mable Lam, Samantha E. Ryken, Theresa Wiesner, Kimberly Lukasik, Bradley J Zuchero, Christophe Leterrier, David Adalsteinsson, Stephanie L. Gupton