Microscopy preprints – applications in cell biology and more

Posted by FocalPlane, on 14 January 2022

Here is a curated selection of preprints published recently. In this post, we focus specifically on preprints using microscopy tools in different fields such as cell biology, plant biology, microbiology, and neuroscience.

Nanoscale details of mitochondrial fission revealed by cryo-electron tomography. Shrawan Kumar Mageswaran, Danielle Ann Grotjahn, Xiangrui Zeng, Benjamin Asher Barad, Michaela A Medina, My Hanh Hoang, Megan J Dobro, Yi-Wei Chang, Min Xu, Wei Yuan Yang, Grant J Jensen

Figure panels extracted from Kumar *et al.*

Islet primary cilia motility controls insulin secretion. Jung Hoon Cho, Zipeng A Li, Lifei Zhu, Brian Muegge, Henry Roseman, Toby Utterback, Louis Woodhams, Philip V Bayly, Jing Hughes

Figure panels extracted from Cho *et al.*

Entangled architecture of rough endoplasmic reticulum (RER) and vacuoles enables topological damping in cytoplasm of an ultra-fast giant cell. Ray Chang, Manu Prakash

Figure panels extracted from Chang *et al.*

Precise measurement of nanoscopic septin ring structures in deep learning-assisted quantitative superresolution microscopy. Amin Zehtabian, Paul Markus Müller, Maximilian Goisser, Leon Obendorf, Lea Jänisch, Nadja Hümpfer, Jakob Rentsch, Helge Ewers

Figure extracted from Zehtabian *et al.*

Free diffusion of PI(4,5)P2 in the plasma membrane in the presence of high density effector protein complexes. Jonathan Pacheco, Anna C Cassidy, James P Zewe, Rachel C Wills, Gerald R Hammond

Microtubule Lattice Spacing Governs Cohesive Envelope Formation of Tau Family Proteins. Valerie Siahaan, Ruensern Tan, Tereza Humhalova, Lenka Libusova, Samuel E. Lacey, Tracy Tan, Mariah Dacy, Kassandra M. Ori-McKenney, Richard J. McKenney, Marcus Braun, Zdenek Lansky

Control of Leaf Vein Patterning by Regulated Plasmodesma Aperture. Nguyen Manh Linh, Enrico Scarpella

Deep-learning microscopy image reconstruction with quality control reveals second-scale rearrangements in RNA polymerase II clusters. Hamideh Hajiabadi, Irina Mamontova, Roshan Prizak, Agnieszka Pancholi, Anne Koziolek, Lennart Hilbert

Figure extracted from Hajiabadi *et al.*

Dynamics of CTCF and cohesin mediated chromatin looping revealed by live-cell imaging. Michele Gabriele, Hugo B Brandão, Simon Grosse-Holz, Asmita Jha, Gina M Dailey, Claudia Cattoglio, Tsung-Han S Hsieh, Leonid Mirny, Christoph Zechner, Anders Sejr Hansen

Unraveling the kinetochore nanostructure in Schizosaccharomyces pombe using multi-color single-molecule localization microscopy. David Virant, Ilijana Vojnovic, Jannik Winkelmeier, Marc Endesfelder, Bartosz Turkowyd, David Lando, Ulrike Endesfelder

Nanoscale structural organization and stoichiometry of the budding yeast kinetochore. Konstanty Cieslinski, Yu-Le Wu, Lisa Nechyporenko, Sarah Janice Hörner, Duccio Conti, Jonas Ries

Figure extracted from Cieslinski *et al.*

Intra-colony channel morphology in Escherichia coli biofilms is governed by nutrient availability and substrate stiffness. Beatrice Bottura, Liam M Rooney, Paul A Hoskisson, Gail McConnell

Signalling centre vortices coordinate collective behaviour in social amoebae. Hugh Z Ford, Angelika Manhart, Jonathan R Chubb

Neuronal circuits integrating visual motion information in Drosophila. Kazunori Shinomiya, Aljoscha Nern, Ian Meinertzhagen, Stephen M Plaza, Michael B Reiser

Figure extracted from Shinomiya *et al.*

Nanoscale architecture and coordination of actin cores within the sealing zone of human osteoclasts. Marion Portes, Thomas Mangeat, Natacha Escalier, Brigitte Raynaud-Messina, Christophe Thibault, Isabelle Maridonneau-Parini, Christel Vérollet, Renaud Poincloux

Mechanics of human embryo compaction. Julie Firmin, Nicolas Ecker, Diane Rivet Danon, Virginie Barraud Lange, Herve Turlier, Catherine Patrat, Jean-Leon Maitre