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Displaying posts in the category: Education

Etch A Cell - segmenting electron microscopy data with the power of the crowd

Posted by , on 16 July 2020

Recent years have seen remarkable developments in imaging techniques and technologies, producing increasingly rich datasets that require huge amounts of costly technological infrastructure, computational power and researcher effort to process. Techniques such as light-sheet microscopy and volume electron microscopy routinely generate terabytes worth of data overnight. With a single data acquisition producing more images than

Primers on Microscopy for Biologists - Resolution

Posted by , on 15 July 2020

Formal definitions of resolution refer to imaginary objects such as infinitely small sources of light. I will avoid those and instead try to provide a pragmatic explanation. Practically, the spatial resolution is the size of the smallest structure that can be distinguished in the light coming from a specimen. All sensors and components of the

The cell biologist's guide to super-resolution microscopy

Posted by , on 1 July 2020

Super-resolution microscopy has allowed to resolve cellular structures down to the nanoscale. While extremely beneficial for most cell biologists, the advent of this technology has made it somewhat difficult for cell biologists and microscopists alike to select the techniques best suited for their experiments. Guillaume Jacquemet, Alexandre Carisey, Hellyh Hamidi and two members of our

Lessons learned from an open-hardware project in microscopy: The mesoSPIM initiative

Posted by , on 1 July 2020

The mesoscale selective plane illumination microscope (mesoSPIM) is a highly versatile open-source light-sheet microscope for imaging large (cm-sized) cleared tissue samples. Using the documentation and software available via mesospim.org and on Github, interested labs can set up their own microscope.  Currently, ten mesoSPIM instruments are in operation around the globe and several more are under