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Upcoming webinar: A Deep Dive into Biomolecular Condensates Using Single-Molecule FRET

Sponsored by PicoQuant, on 22 November 2023

In a brand-new paper, Ashish Joshi and colleagues from the team of Samrat Mukhopadhyay at IISER Mohali take a close look at the molecular events during phase separation of the FUS protein with single-molecule fluorescence methods. If you want to learn more about these techniques and what they revealed about the complex behavior of the
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Featured image with Nick Gatford

Posted by , on 16 November 2023

Our featured image shows a single human dopaminergic neuron generated from a human stem cell, acquired via super-resolution Airyscan confocal microscopy at the University of Oxford Micron facility by Nick Gatford. We caught up with Nick to find out about his research and what he is excited about in microscopy.
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Featured image with Oona Paavolainen

Posted by , on 15 November 2023

Our featured image, acquired by Oona Paavolainen, shows a primary mammary gland organoid, in a collagen-I matrix, formed from a single primary epithelial cell isolated from material obtained from a breast reduction surgery. The image was taken with a spinning disk confocal microscope (3i CSU-W1 Spinning disk) using a 20x Zeiss Plan-Apochromat (NA=0.8) objective. The
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Featured image with Till Stephan

Posted by , on 14 November 2023

Our featured image, acquired by Till Stephan, shows a live-cell stimulated emission depletion (STED) microscopy recording of a HeLa cell in which mitochondria (orange-magenta) and microtubules (cyan) are labelled. The super-resolution image reveals the densely stacked mitochondrial cristae, coordinated invaginations of the mitochondrial inner membrane.
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New look for FocalPlane

Posted by , on 13 November 2023

We are delighted to launch a new look for FocalPlane. Every day this week, we’ll be releasing a new banner image and highlighting the scientist that acquired it. Today, we start with an image from Rebecca Simkin. Rebecca won our 2023 image competition, and you can read our featured image post with Rebecca here: https://focalplane.biologists.com/2023/09/01/and-the-winner-is/
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New partnership with MicroscopyDB

Posted by , on 30 October 2023

We are delighted to announce our new partnership with MicroscopyDB – a global repository of online microscopy resources. This new partnership means that the listings on our job and events boards will be pulled in from the MicroscopyDB database, ensuring that FocalPlane readers can keep up to date with event and job listings across the
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Featured image with Ioakeim (Makis) Ampartzidis

Posted by , on 27 October 2023

Our featured image, ‘Dandelion in spring’ from Ioakeim (Makis) Ampartzidis, shows neuroepithelial-like cells cultured on glass beads. The cell nuclei are coloured in magenta, and the cytoskeleton is stained in black, labelling F-actin. The actively proliferating cells are highlighted with Ki-67 antigen in green. We caught up with Makis to find out more about his
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Featured image with Victoria Alonso

Posted by , on 13 October 2023

Our featured image is a Trypanosoma cruzi epimastigote, prepared for expansion confocal microscopy with pan-proteome staining with NHS-ester Atto564 by Victoria Alonso. The image is a z-stack acquired with a Zeiss LSM 880 and was edited with ImageJ Fiji, plugin: Z-stack depth Colorcode.
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Becoming ‘Greener’ with Light Microscopy

Sponsored by CoolLED, on 11 October 2023

Working in a laboratory, you can’t help but notice how everyday tasks can impact the environment – from reliance on single-use plastics to energy-hungry equipment. While this might seem unavoidable, sustainable options are becoming widely available. In fact, many technologies and initiatives now exist to support the scientific community on the journey towards sustainable laboratories.  
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Featured image with Peggy Paschke

Posted by , on 29 September 2023

Our featured image is called Dictyostelium Trees on Fire and was acquired by Peggy Paschke. The image shows Dictyostelium discoideum cells stably expressing the cAMP sensor Flamindo2. Cells were imaged as 5×5 tile scan for 5 hours with a Nikon AXR (488 nm excitation) using a 4x air objective. Shown is a cropped part of
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