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New insights into corneal development from vEM

Posted by , on 17 June 2025

by Rob Young*, Kiranjit Bains*, Phil Lewis* and Andrew Quantock* *Biophysics Research Group, School of Optometry & Vision Sciences, Cardiff University, UK Challenge The unique transparency of corneal stromal tissue, essential for vision, is dependent upon the remarkable order of component collagen fibrils, which exhibit highly uniform diameter, spacing and orientation. However, processes involved in
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International Microscopy Facility Survey

Posted by , on 9 November 2024

Some of you may have seen this already (thanks if you have already filled this in). I have been running an International Microscopy Facility Survey over the last few months. It is based on the excellent BioImaging UK survey that was published a few years ago, but with an extended focus to include Optical Microscopy,
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vEM reveals the cellular composition of the Blood Nerve Barrier

Posted by , on 11 October 2024

Using Serial Block-Face-SEM6 and AT-SEM, we characterized the cellular composition and interactions along EndoBVs7. Unlike the BBB, we observed that the vasculature within the endoneurium is not completely covered. Upon closer examination of the cells in contact with EndoBVs, we identified three distinct cell types frequently interacting with the vasculature.
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And the winner is…

Posted by , on 7 June 2024

In an exciting turn of events, we are delighted to announce that the winner of the FocalPlane/elmi (European Light Microscopy Initiative) is the electron micrograph, ‘Glorious Lymphocyte’ from Rossana Melo! The image shows a leukocyte (lymphocyte) trapped among aggregates of red blood cells in a small lung vessel of a patient with asthma. The image
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FocalPlane features... microscopy-based outreach

Posted by , on 11 March 2024

The first webinar of our new series of FocalPlane features... focussed on microscopy-based outreach. We hosted fantastic talks from Paola Moreno-Roman from Foldscope Instruments and Martin Jones from The Francis Crick Institute and we share the recordings here.
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An interview on the Traversing European Coastlines (TREC) project with Yannick Schwab

Posted by , on 5 March 2024

Traversing European Coastlines (TREC) is a flagship project in the planetary biology theme from European Molecular Biology Laboratory (EMBL). A consortium of several research organisations under the lead of EMBL has embarked on an expedition to sample the biodiversity in the coastlines of Europe in 2023 and 2024. We caught up with Yannick Schwab, Team
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Imaging with the Instituto Gulbenkian de Ciência’s Electron Microscopy Facility

Posted by , on 14 December 2023

In our ‘Imaging with…’ blog post, we meet the staff of the Electron Microscopy (EM) facility at the Instituto Gulbenkian de Ciência, Lisbon, Portugal.
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An interview with Kildare Miranda

Posted by , on 10 May 2022

Kildare Miranda is the director of the Advanced Microscopy Unit and vice-director of the National Centre for Structural Biology and Bioimaging (CENABIO), Professor of Biophysics and Cell Biology and Head of the Cell Biology and Parasitology Department of the Biophysics Institute at the Federal University of Rio de Janeiro, and holds several CNPq fellowships. He
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An interview with Moara Lemos

Posted by , on 3 May 2022

Moara Lemos is currently a permanent researcher at Institut Pasteur in France, where she has helped establish cryo-correlative light and electron microscopy and cellular cryo-tomography for different biological systems. She began her career (BSc and MSc) at the Federal University of Juiz de For a, working on trypanosomes of wild animals. She later did her
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How volumeEM (vEM) can help map neuronal circuits

Posted by , on 2 March 2022

by Nadine Randel, University of Cambridge, UK DOI:10.5281/zenodo.6320414 Challenge The nervous system processes sensory information and generates different behavioural outputs accordingly. Synaptic resolution wiring diagrams form a basis for our understanding of how the brain works. Such a connectome provides the synaptic connectivity between all neurons and allows to identify all potential neuronal pathways which
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