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New look for FocalPlane

Posted by , on 13 November 2023

We are delighted to launch a new look for FocalPlane. Every day this week, we’ll be releasing a new banner image and highlighting the scientist that acquired it. Today, we start with an image from Rebecca Simkin. Rebecca won our 2023 image competition, and you can read our featured image post with Rebecca here: https://focalplane.biologists.com/2023/09/01/and-the-winner-is/
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Featured image with Ioakeim (Makis) Ampartzidis

Posted by , on 27 October 2023

Our featured image, ‘Dandelion in spring’ from Ioakeim (Makis) Ampartzidis, shows neuroepithelial-like cells cultured on glass beads. The cell nuclei are coloured in magenta, and the cytoskeleton is stained in black, labelling F-actin. The actively proliferating cells are highlighted with Ki-67 antigen in green. We caught up with Makis to find out more about his
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Featured image with Victoria Alonso

Posted by , on 13 October 2023

Our featured image is a Trypanosoma cruzi epimastigote, prepared for expansion confocal microscopy with pan-proteome staining with NHS-ester Atto564 by Victoria Alonso. The image is a z-stack acquired with a Zeiss LSM 880 and was edited with ImageJ Fiji, plugin: Z-stack depth Colorcode.
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Featured image with Peggy Paschke

Posted by , on 29 September 2023

Our featured image is called Dictyostelium Trees on Fire and was acquired by Peggy Paschke. The image shows Dictyostelium discoideum cells stably expressing the cAMP sensor Flamindo2. Cells were imaged as 5×5 tile scan for 5 hours with a Nikon AXR (488 nm excitation) using a 4x air objective. Shown is a cropped part of
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And the winner is...

Posted by , on 1 September 2023

We are delighted to announce that the winner of the 2023 FocalPlane image competition is Rebecca Simkin. Rebecca’s image, ‘Neuromuscular junctions’, depicts four NMJs in a lumbrical muscle, located in the hind paw of a wildtype mouse. A wholemount immunostaining was performed using 2H3 and SV2 antibodies to visualise lower motor neurons in green (specifically
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Featured image with Beste Senem Değirmenci

Posted by , on 18 August 2023

Our featured image, ‘Bubbles of The Cell’, shows enlarged endosomal vesicles in HeLa S3 cells upon inhibition of PI(3,5)P2 activity. PI(3,5)P2 is a crucial phosphoinositide involved in the regulation of endosomal trafficking. Depletion of PI(3,5)P2 disrupts the transition from early to late endosomes, leading to the enlargement of endosomal vesicles. Imaged with Leica DMI8 SP8
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Featured image with Outi Paloheimo

Posted by , on 4 August 2023

Our featured image is laser scanning confocal microscope image showing cell division of Madin-Darby canine kidney cells with nuclei in grey and filamentous actin in orange. The image was acquired with a Zeiss LSM 780 microscope with a 63x/1.40 oil immersion objective, 405 nm diode laser and 561 nm diode-pumped solid-state laser. Deconvolution was performed
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Featured image with David McGrath

Posted by , on 21 July 2023

Our featured image is of mature mouse brown adipocytes (WT-1 cell line) under normal conditions from David McGrath. Channel 1 (green LUT) shows lipid droplets stained with BODIPY 493/503 and channel 2 (fire LUT) shows mitochondria stained with TMRE. The image was obtained using a Visitron SD-TIRF confocal microscope (60x Apo TIRF (corr.) Oil/ NA: 1.49) at the UKE Microscopy Imaging Facility
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Featured image with Ramiro Tomasina

Posted by , on 12 July 2023

Our featured image shows an ultrastructure expansion microscopy image of a procyclic Trypanosoma brucei labelled with anti-acetylated tubulin in magenta, DAPI in cyan and a flagellar marker in yellow. The image is a z-projection of  maximum intensity. The image was acquired in the Trypanosoma cell biology unit at the Institut Pasteur of Paris (headed by Philippe Bastin),
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Featured image with Simon Desiderio

Posted by , on 21 April 2023

Our featured image shows the developing dorsal root ganglia of an E12.5 mouse embryo from Simon Desiderio.
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