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VolumeEM: An Interview with Kedar Narayan

Posted by , on 14 December 2024

I had the pleasure of sitting down with recent awardee of the Alan Agar Royal Microscopy Award for Electron Microscopy, Dr. Kedar Narayan. Dr. Narayan is very involved with the grassroots Volume EM (vEM) community, and has used his time at Frederick National Laboratory to not only benefit cancer research, but microscopy technique as a
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Featured image with Jiwon Lee

Posted by , on 6 December 2024

Our featured image, acquired by Jiwon Lee, is a 3D rendering model of Plasmodium falciparum showing the complex organisation of cellular organelles within the malaria parasite. Using a focused ion beam scanning electron microscope (FIB-SEM), a series of high resolution 2D images were acquired. The image stack was aligned and each organelle was segmented in
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Microscopy preprints - bioimage analysis

Posted by , on 29 November 2024

Here is a curated selection of preprints published recently. In this post, we focus specifically on bioimage analysis and data management.
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FocalPlane features... Imaging Cell Architecture and Dynamics with Journal of Cell Science

Posted by , on 15 November 2024

Our latest FocalPlane features… webinar was a celebration of the research in Journal of Cell Science’s Special Issue: Imaging Cell Architecture and Dynamics. Our Guest Editors Lucy Collinson and Guillaume Jacquemet hosted the webinar with talks from Md Hashim Reza, Melanie Rug and Vikas Tillu.
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Journal of Cell Science Special Issue: Imaging Cell Architecture and Dynamics

Posted by , on 5 November 2024

Journal of Cell Science’s Special Issue on Imaging Cell Architecture and Dynamics is now complete. The original research in this Issue, guest edited by Lucy Collinson and Guillaume Jacquemet, covers a diverse range of imaging methodologies including CLEM, super-resolution imaging, volume EM, high-throughput imaging, FRET, expansion microscopy, mesoscopy and immuno-SEM, as well as showcasing new
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vEM reveals the cellular composition of the Blood Nerve Barrier

Posted by , on 11 October 2024

Using Serial Block-Face-SEM6 and AT-SEM, we characterized the cellular composition and interactions along EndoBVs7. Unlike the BBB, we observed that the vasculature within the endoneurium is not completely covered. Upon closer examination of the cells in contact with EndoBVs, we identified three distinct cell types frequently interacting with the vasculature.
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Unveiling the Marvels of the First-Ever Gordon Research Conference on Volume Electron Microscopy - Capturing Subcellular Architecture from Organelles to Organisms -By Ana Correia and James Thompson

Posted by , on 24 August 2023

We delve into our captivating journey at the first Gordon Research Conference (GRC) on Volume Electron Microscopy (vEM) as early career scientists. Set against the backdrop of sunny Ventura, California, this conference brought together experts in the vEM field from all corners of the globe, creating an intellectual haven for learning, networking, and forging collaborations.
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Inputs and Outputs of vEM in a Sensory System

Posted by , on 19 May 2023

by Federica Mangione, The Francis Crick Institute, UK DOI: 10.1242/focalplane.14809 Challenge The sense of touch enables organisms to interact with their environment by perceiving physical forces and guiding complex behaviors. Touch sensing is mediated by sensory neurons that innervate the body surface of animals and, together with surrounding cells, form specialized structures known as tactile
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