Microscopy preprints – applications in cell biology and more

Posted by FocalPlane, on 8 April 2022

Here is a curated selection of preprints published recently. In this post, we focus specifically on preprints using microscopy tools in different fields such as cell biology, plant biology, microbiology, and neuroscience.

Three-dimensional cilia structures from animals’ closest unicellular relatives, the Choanoflagellates

Justine M. Pinskey, Adhya Lagisetty, Long Gui, Nhan Phan, Evan Reetz, Gang Fu, Daniela Nicastro

Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assembly

Jurica Matković, Subhadip Ghosh, Mateja Ćosić, Nenad Pavin, Iva M. Tolić

Temporally extended and reversible neuronal silencing with Aion

Silvia Rodriguez-Rozada, Jonas Wietek, Federico Marcello Tenedini, Kathrin Sauter, Peter Hegemann, Peter Soba, J. Simon Wiegert

Figure extracted from Rodriguez-Rozada *et al.*

Cyclin CLB2 mRNA localization determines efficient protein synthesis to orchestrate bud growth and cell cycle progression

Evelina Tutucci, Anna Maekiniemi, Jacky L Snoep, Markus Seiler, Kelly van Rossum, David D. van Niekerk, Philipp Savakis, Kathi Zarnack, Robert H Singer

Synaptotagmin 1 mediates toxicity of botulinum neurotoxin type A

Merja Joensuu, Vanessa Lanoue, Parnayan Syed, Tristan P. Wallis, James Rae, Ailisa Blum, Rachel Gormal, Christopher Small, Shanley Sanders, Anmin Jiang, Stefan Mahrhold, Nadja Krez, Michael A. Cousin, Ruby Cooper-White, Justin J. Cooper-White, Brett A. Collins, Robert G Parton, Giuseppe Balistreri, Andreas Rummel, Frederic A Meunier

Ultrastructure Expansion Microscopy (U-ExM) in Trypanosoma cruzi: localization of tubulin isoforms and isotypes

Victoria Lucia Alonso

Tension at intercellular junctions is necessary for accurate orientation of cell division in the epithelium plane

Ana Lisica, Jonathan Fouchard, Manasi Kelkar, Tom P. J. Wyatt, Julia Duque, Anne-Betty Ndiaye, Alessandra Bonfanti, Buzz Baum, Alexandre J. Kabla, Guillaume T. Charras

A versatile pattern-forming cortical circuit based on Rho, F-actin, Ect2, and RGA-3/4

Ani Michaud, Marcin Leda, Zachary T. Swider, Songeun Kim, Jiaye He, Jennifer Landino, Jenna R. Valley, Jan Huisken, Andrew B. Goryachev, George von Dassow, William Bement

In situ architecture of the lipid transport protein VPS13C at ER-lysosomes membrane contacts

Shujun Cai, Yumei Wu, Andres Guillen-Samander, William F Hancock-Cerutti, Jun Liu, Pietro De Camilli

Ultrastructure of COPII vesicle formation characterised by correlative light and electron microscopy

Alejandro Melero, Jerome Boulanger, Wanda Kukulski, Elizabeth A. Miller

Imaging tools generated by CRISPR/Cas9 tagging reveal cytokinetic diversity in mammalian cells

Mathieu C. Husser, Imge Ozugergin, Tiziana Resta, Vincent J J. Martin, Alisa J. Piekny

A role of Lck annular lipids in the steady upkeep of active Lck in T cells

Nicla Porciello, Deborah Cipria, Giulia Masi, Anna-Lisa Lanz, Edoardo Milanetti, Alessandro Grottesi, Duncan Howie, Steve P. Cobbold, Lothar Schermelleh, Hai-Tao He, Marco D’Abramo, Nicolas Destainville, Oreste Acuto, Konstantina Nika

Figure extracted from Porciello *et al.*

A conformational switch in clathrin light chain regulates lattice structure and endocytosis at the plasma membrane of mammalian cells

Kazuki Obashi, Kem A. Sochacki, Marie-Paule Strub, Justin W. Tarasaka

Microtubules self-repair in living cells

Morgan Gazzola, Alexandre Schaeffer, Benoit Vianay, Jérémie Gaillard, Laurent Blanchoin, Manuel Théry

Disruption of the microphysiological niche alters matrix deposition and causes loss of mitochondrial Ca²⁺ control in skeletal muscle fibers

Charlotte Gineste , Sonia Youhanna, Sabine U. Vorrink, Sara Henriksson, Andrés Hernández, Arthur J. Cheng, Thomas Chaillou, Andreas Buttgereit, Dominik Schneidereit, Oliver Friedrich, Kjell Hultenby, Joseph D. Bruton, Niklas Ivarsson, Linda Sandblad, Volker M. Lauschke, Håkan Westerblad