Microscopy preprints – new tools and techniques in imaging

Posted by FocalPlane, on 23 February 2024

Here is a curated selection of preprints posted recently on new tools and techniques in imaging. Let us know if we are missing any recent preprints that are on your reading list!

Instant square lattice structured illumination microscopy: an optimal strategy towards photon-saving and real-time super-resolution observation
Tianyu Zhao, Zhaojun Wang, Manming Shu, Jingxiang Zhang, Yansheng Liang, Shaowei Wang, Ming Lei

3D orientation super-resolution spatial-frequency-shift microscopy
Xiaowei Liu, Mingwei Tang, Ning Zhou, Chenlei Pang, Zhong Wen, Xu Liu, Qing Yang

OpenSIM: open source microscope add-on for structured illumination microscopy
Mélanie T.M Hannebelle, Esther Raeth, Samuel M. Leitao, Tomáš Lukeš, Jakub Pospíšil, Chiara Toniolo, Olivier F. Venzin, Antonius Chrisnandy, Prabhu Prasad Swain, Nathan Ronceray, Matthias P. Lütolf, Andrew C. Oates, Guy M. Hagen, Theo Lasser, Aleksandra Radenovic, John D. McKinney, Georg E. Fantner

Spectrally Resolved Localization Microscopy with Ultrahigh-Throughput
James Ethan Batey, Geun Wan Kim, Meek Yang, Darby Claire Heffer, Elric Dion Pott, Hannah Giang, Bin Dong

Gentle rhodamines for live-cell fluorescence microscopy
Tianyan Liu, Julian Kompa, Jing Ling, Nicolas Lardon, Yuan Zhang, Jingting Chen, Luc Reymond, Peng Chen, Mai Tran, Zhongtian Yang, Haolin Zhang, Yitong Liu, Stefan Pitsch, Peng Zou, Lu Wang, Kai Johnsson, Zhixing Chen

Figure extracted from Liu, *et al.* made available under a CC-BY-NC 4.0 International license.

navigate: an open-source platform for smart light-sheet microscopy
Zach Marin, Xiaoding Wang, Dax W. Collison, Conor McFadden, Jinlong Lin, Hazel Borges, Bingying Chen, Dushyant Mehra, Qionghua Shen, Seweryn Galecki, Stephan Daetwyler, Reto Fiolka, Kevin M. Dean

Root Expansion Microscopy (ROOT-ExM): A streamlined super resolution method for plants
Magali S GRISON, Guillaume Maucort, Amandine Dumazel, Dorian Champelovier, Yohann BOUTTE, Monica Fernandez-Monreal, Emmanuelle Bayer

Super-resolution expansion microscopy in plant roots
Michelle Gallei, Sven Truckenbrodt, Caroline Kreuzinger, Syamala Inumella, Vitali Vistunou, Christoph Sommer, Mojtaba R. Tavakoli, Nathalie Agudelo-Dueñas, Jakob Vorlaufer, Wiebke Jahr, Marek Randuch, Alexander Johnson, Eva Benková, Jiří Friml, Johann G. Danzl

Figure extracted from Gallei, *et al.* made available under a CC-BY-NC 4.0 International license.

Far-red chemigenetic biosensors for multi-dimensional and super-resolved kinase activity imaging
Michelle S. Frei, Samantha A. Sanchez, Longwei Liu, Falk Schneider, Zichen Wang, Hiroyuki Hakozaki, Yajuan Li, Anne C. Lyons, Theresa V. Rohm, Jerrold M. Olefsky, Lingyan Shi, Johannes Schöneberg, Scott E. Fraser, Sohum Mehta, Yingxiao Wang, Jin Zhang

Super-Photostable Organic Dye for Long-Term Live-cell Single Protein Imaging
Sung Ho Ryu, Do-Hyeon Kim, Minh Triet Hong, Sun Hyeok Lee, Sina Jazani, Seongjae Jang, Jongcheol Seo, Taekjip Ha,Young-Tae Chang

Figure extracted from Ryu, *et al.* made available under a CC-BY 4.0 International license.

Squid: Simplifying Quantitative Imaging Platform Development and Deployment
Hongquan Li, Deepak Krishnamurthy, Ethan Li, Pranav Vyas, Nibha Akireddy, Chew Chai, Manu Prakash

Diffraction minima resolve point scatterers at tiny fractions (1/80) of the wavelength
Thomas A. Hensel, Jan O. Wirth, Stefan W. Hell

EasyGrid: A versatile platform for automated cryo-EM sample preparation and quality control
Olivier Gemin, Victor Armijo, Michael Hons, Caroline Bissardon, Romain Linares, Matthew W. Bowler, Georg Wolff, Kirill Kovalev, Anastasiia Babenko, Veijo T. Salo, Sarah Schneider, Christopher Rossi, Léa Lecomte, Thibault Deckers, Kévin Lauzier, Robert Janocha, Franck Felisaz, Jérémy Sinoir, Wojciech Galej, Julia Mahamid, Christoph W. Müller, Sebastian Eustermann, Simone Mattei, Florent Cipriani, Gergely Papp

Figure extracted from Gemin, *et al.* made available under a CC-BY 4.0 International license.

Cryosectioning-enabled super-resolution microscopy for studying nuclear architecture at the single protein level
Johannes Stein, Maria Ericsson, Michel Nofal, Lorenzo Magni, Sarah Aufmkolk, Ryan B. McMillan, Laura Breimann, Conor P. Herlihy, S. Dean Lee, Andréa Willemin, Jens Wohlmann, Laura Arguedas-Jimenez, Peng Yin, Ana Pombo, George M. Church, Chao-ting Wu

Yellow and oxidation-resistant derivatives of a monomeric superfolder GFP
Fernando M. Valbuena, Adam H. Krahn, Sherzod A. Tokamov, Annie C. Greene, Richard G. Fehon, Benjamin S. Glick

Super-resolved protein imaging using bifunctional light-up aptamers
Franziska Grün, Niklas van den Bergh, Maja Klevanski, Mrigank S. Verma, Bastian Bühler, G. Ulrich Nienhaus, Thomas Kuner, Andres Jäschke, Murat Sunbul

Standardised Measurements for Monitoring and Comparing Multiphoton Microscope Systems
Robert M. Lees, Isaac H. Bianco, Robert A.A. Campbell, Natalia Orlova, Darcy S. Peterka, Bruno Pichler, Spencer LaVere Smith, Dimitri Yatsenko, Che-Hang Yu, Adam M. Packer

Deep super-resolution imaging of thick tissue using structured illumination with adaptive optics
Jingyu Wang, Danail Stoychev, Mick Phillips, David Miguel Susano Pinto, Richard M. Parton, Nick Hall, Josh Titlow, Ana Rita Faria, Matthew Wincott, Dalia Gala, Andreas Gerondopoulos, Niloufer Irani, Ian Dobbie, Lothar Schermelleh, Martin Booth, Ilan Davis

Doubling the resolution of fluorescence-lifetime single-molecule localization microscopy with image scanning microscopy
Niels Radmacher, Oleksii Nevskyi, José Ignacio Gallea, Jan Christoph Thiele, Ingo Gregor, Silvio O. Rizzoli, Jörg Enderlein

ESPressoscope: a small and powerful platform for in situ microscopy
Ethan Li, Vittorio Saggiomo, Wei Ouyang, Manu Prakash, Benedict Diederich

Figure extracted from Li, *et al.* made available under a CC-BY 4.0 International license.

Depth-dependent scaling of axial distances in light microscopy
S.V. Loginov, D.B. Boltje, M.N.F. Hensgens, J.P. Hoogenboom, E.B. Van Der Wee

A practical guide to time-resolved fluorescence microscopy and spectroscopy
Benjamin S. Clark, Irene Silvernail, Kenya Gordon, Andi N. Morgan, Lewis A. Rolband, Jose F. Castaneda, Sharonda J. LeBlanc

High-speed two-photon microscopy with adaptive line-excitation
Yunyang Li, Shu Guo, Ben Mattison, Weijian Yang

Bio-orthogonal Glycan Imaging of Culture Cells and Whole Animal C. elegans with Expansion Microscopy
Joe Chin-Hun Kuo, Marshall J. Colville, Michelle R. Sorkin, Jacky Lok Ka Kuo, Ling Ting Huang, Dana N. Thornlow, Gwendolyn M. Beacham, Gunther Hollopeter, Matthew P. DeLisa, Christopher A. Alabi, Matthew J. Paszek

Using splines for point spread function calibration at non-uniform depths in localization microscopy
Serafim Korovin, Dylan Kalisvaart, Shih-Te Hung, Jelmer Cnossen, Coen de Visser, Carlas S. Smith

Bridging the resolution-sectioning gap in stimulated emission depletion microscopy: theory and experiment
Michael Belsley, Joana Soares-de-Oliveira, António J. Pereira

Astigmatism-based focus stabilisation with universal objective lens compatibility, extended operating range and nanometre precision
Amir Rahmani, Tabitha Cox, Akhila Thamaravelil Abhimanue Achary, Aleks Ponjavic

An open-source, high resolution, automated fluorescence microscope
Ando C. Zehrer, Ana Martin-Villalba, Benedict Diederich, Helge Ewers

Photo-Uncaging Triggers on Self-Blinking to Control Single-Molecule Fluorescence Kinetics for Super-Resolution Imaging
Ying Zheng, Zhiwei Ye, Xue Zhang, Yi Xiao

Trackoscope: A Low-Cost, Open, Autonomous Tracking Microscope for Long-Term Observations of Microscale Organisms
Priya Soneji, Elio J. Challita, M. Saad Bhamla

Simultaneous multicolor fluorescence imaging using duplication-based PSF splitting
Robin Van den Eynde, Fabian Hertel, Sergey Abakumov, Bartosz Krajnik, Siewert Hugelier, Alexander Auer, Joschka Hellmeier, Thomas Schlichthaerle, Rachel M. Grattan, Diane S. Lidke, Ralf Jungmann, Marcel Leutenegger, Wim Vandenberg, Peter Dedecker