Microscopy preprints – new tools and techniques in imaging

Posted by FocalPlane, on 17 May 2024

Here is a curated selection of preprints posted recently on new tools and techniques in imaging. Let us know if we are missing any recent preprints that are on your reading list!

Fast adaptive super-resolution lattice light-sheet microscopy for rapid, long-term, near-isotropic subcellular imaging
Chang Qiao, Ziwei Li, Zongfa Wang, Yuhuan Lin, Chong Liu, Siwei Zhang, Yong Liu, Yun Feng, Xinyu Wang, Xue Dong, Jiabao Guo, Tao Jiang, Qinghua Wang, Qionghai Dai, Dong Li

Curved light sheet microscopy for centimeter-scale cleared tissues imaging
Lijuan Tang, Jiayu Wang, Jiayi Ding, Junyou Sun, Xing-jun Chen, Quqing Shen, Ruiheng Song, Peng Cao, Rong Gong, Fang Xu, Woo-ping Ge, Wenzhi Sun, Hu Zhao, Jianglai Wu

Direct single-molecule detection and super-resolution imaging with a low-cost portable smartphone-based microscope
Morgane Loretan, Mariano Barella, Nathan Fuchs, Samet Kocabey, Karol Kołątaj, Fernando D. Stefani, Guillermo P. Acuna

POLCAM: Instant molecular orientation microscopy for the life sciences
Ezra Bruggeman, Oumeng Zhang, Lisa-Maria Needham, Markus Körbel, Sam Daly, Matthew Cheetham, Ruby Peters, Tingting Wu, Andrey S. Klymchenko, Simon J. Davis, Ewa K. Paluch, David Klenerman, Matthew D. Lew, Kevin O’Holleran, Steven F. Lee

Motion Blur Microscopy
Utku Goreke, Ayesha Gonzales, Brandon Shipley, Madeleine Tincher, Oshin Sharma, William Wulftange, Yuncheng Man, Ran An, Michael Hinczewski, Umut A Gurkan

Mechanically Sheared Axially Swept Light-Sheet Microscopy
Jinlong Lin, Dushyant Mehra, Zach Marin, Xiaoding Wang, Hazel M. Borges, Qionghua Shen, Seweryn Gałecki, John Haug, Kevin M. Dean

“A Low-Cost Microfluidics System for Light Microscopy Experiments”
Logan M. Harrison, Benjamin M. Brisard, Kylie D. Cashwell, Aaron L. Mulkey, Cameron A. Schmidt

Dose-Efficient Cryo-Electron Microscopy for Thick Samples using Tilt-Corrected Scanning Transmission Electron Microscopy, Demonstrated on Cells and Single Particles
Yue Yu, Katherine A. Spoth, Michael Colletta, Kayla X. Nguyen, Steven E. Zeltmann, Xiyue S. Zhang, Mohammadreza Paraan, Mykhailo Kopylov, Charlie Dubbeldam, Daniel Serwas, Hannah Siems, David A. Muller, Lena F. Kourkoutis

Maximizing photon utilization in spectroscopic single-molecule localization microscopy using symmetrically dispersed dual-wedge prisms
Wei-Hong Yeo, Benjamin Brenner, Youngseop Lee, Junghun Kweon, Cheng Sun, Hao F. Zhang

Figure extracted from Yeo, *et al.* made available under a CC-BY-NC 4.0 International license.

Extended-depth of field random illumination microscopy, EDF-RIM, provides super-resolved projective imaging
Lorry Mazzella, Thomas Mangeat, Guillaume Giroussens, Benoit Rogez, Hao Li, Justine Creff, Mehdi Saadaoui, Carla Martins, Ronan Bouzignac, Simon Labouesse, Jérome Idier, Frédéric Galland, Marc Allain, Anne Sentenac, Loïc LeGoff

Figure extracted from Mazzella, *et al.* made available under a CC-BY 4.0 International license.

Time-lapse Image Super-resolution Neural Network with Reliable Confidence Evaluation for Optical Microscopy
Chang Qiao, Shuran Liu, Yuwang Wang, Wencong Xu, Xiaohan Geng, Tao Jiang, Jingyu Zhang, Quan Meng, Hui Qiao, Dong Li, Qionghai Dai

Lightsheet microscopy integrates single-cell optical visco-elastography and fluorescence cytometry of 3D live tissues
Yuji Tomizawa, Khadija H. Wali, Manav Surti, Yasir Suhail, Kshitiz, Kazunori Hoshino

Adaptive optical third-harmonic generation microscopy for in vivo imaging of tissues
Cristina Rodríguez, Daisong Pan, Ryan G. Natan, Manuel A. Mohr, Max Miao, Xiaoke Chen, Trent R. Northen, John P. Vogel, Na Ji

Flat-field super-resolution structured illumination microscopy with joint spatial-temporal light modulation
Yong Liang, Gang Wen, Jie Zhang, Simin Li, Yao Tan, Xin Jin, Linbo Wang, Xiaohu Chen, Jing Gao, Hui Li

Pixel-wise programmability enables dynamic high-SNR cameras for high-speed microscopy
Jie Zhang, Jonathan Newman, Zeguan Wang, Yong Qian, Pedro Feliciano-Ramos, Wei Guo, Takato Honda, Zhe Sage Chen, Changyang Linghu, Ralph Etienne-Cummings, Eric Fossum, Edward Boyden, Matthew Wilson

A posterior approach to correct for focal plane offsets in lattice light sheet structured illumination microscopy
Yu Shi, Tim A. Daugird, Wesley R. Legant

Axial de-scanning using remote focusing in the detection arm of light-sheet microscopy
Hassan Dibaji, Ali Kazemi Nasaban Shotorban, Rachel M Grattan, Shayna Lucero, David J. Schodt, Keith A. Lidke, Jonathan Petruccelli, Diane S. Lidke, Sheng Liu, Tonmoy Chakraborty

Mirror-assisted light-sheet microscopy: a simple upgrade to enable bi-directional sample excitation
Asaph Zylbertal, Isaac H. Bianco

Figure extracted from Zylbertal and Bianco made available under a CC-BY 4.0 International license.

A unique Tandem RAman Microscopy (TRAM) integrating spontaneous and coherent Raman scattering offers integrated data analysis to improve optical biosensing
K. Brzozowski, A. Pieczara, W. Korona, A. Nowakowska, B. Orzechowska, J. Firlej, A. Wislocka-Orlowska, M. Baranska

High Refractive Index Imaging Buffer for Dual Color 3D SMLM Imaging of Thick Samples
Lulu Zhou, Wei Shi, Shuang Fu, Mengfan Li, Jianwei Chen, Ke Fang, Yiming Li

Photochromic reversion enables long-term tracking of single molecules in living plants
Michelle von Arx, Kaltra Xhelilaj, Philip Schulz, Sven zur Oven-Krockhaus, Julien Gronnier

Accelerated protein retention expansion microscopy using microwave radiation
Meghan R. Bullard, Juan Carlos Martinez Cervantes, Norisha B. Quaicoe, Amanda Jin, Danya A. Adams, Jessica M. Lin, Elena Iliadis, Tess M. Seidler, Isaac Cervantes-Sandoval, Hai-yan He

Surface passivation and functionalization for interferometric scattering microscopy
Jenny Sülzle, Laila Elfeky, Suliana Manley

Genetically encoded reporters of actin filament organization in living cells and tissues
Carla Silva Martins, François Iv, Shashi Kumar Suman, Thomas C. Panagiotou, Clara Sidor, María Ruso-López, Camille N. Plancke, Shizue Omi, Maxime Gomes, Alexander Llewellyn, Sourish Reddy Bandi, Laurie Ramond, Federica Arbizzani, Caio Vaz Rimoli, Frank Schnorrer, François Robin, Andrew Wilde, Loïc LeGoff, Jean-Denis Pedelacq, Stéphanie Cabantous, Sergio A. Rincon, Cristel Chandre, Sophie Brasselet, Manos Mavrakis

Figure extracted from Silva Martins, *et al.* made available under a CC-BY 4.0 International license.

A gentle palette of plasma membrane dyes
Jing Ling, Yitong Liu, Yunzhe Fu, Shuzhang Liu, Ling Ding, Lulu Huang, Peng Xi, Zhixing Chen

Figure extracted from Ling, *et al.* made available under a CC-BY 4.0 International license.

Multiplexed in vivo imaging with fluorescence lifetime modulating tags
Lina El Hajji, France Lam, Maria Avtodeeva, Hela Benaissa, Christine Rampon, Michel Volovitch, Sophie Vriz, Arnaud Gautier

A tunable and versatile chemogenetic near infrared fluorescent reporter
Lina El Hajji, Benjamin Bunel, Octave Joliot, Chenge Li, Alison G. Tebo, Christine Rampon, Michel Volovitch, Evelyne Fischer, Nicolas Pietrancosta, Franck Perez, Xavier Morin, Sophie Vriz, Arnaud Gautier

Impact of photobleaching of fluorescent proteins on FRET measurements under two-photon excitation
Dhruba P. Adhikari, Michael R. Stoneman, Valerica Raicu

Lanthanide Cathodophores for Multicolor Electron Microscopy
Sohaib Abdul Rehman, Jeremy B. Conway, Amy Nichols, Edward R. Soucy, Amanda Dee, Kristal Stevens, Simon Merminod, Isabella MacNaughton, Abigail Curtis, Maxim B. Prigozhin

Traction Force Microscopy with DNA FluoroCubes
Armina Mortazavi, Jianfei Jiang, Philip Laric, Dominic A. Helmerich, Rick Seifert, Markus Sauer, Benedikt Sabass

Figure extracted from Mortazavi, *et al.* made available under a CC-BY 4.0 International license.

Novel Genetically Encoded Probes for Functional Imaging of Cell Signaling by Polarization Microscopy
Paul Sebastian Miclea, Vendula Nagy Markova, Alina Sakhi, Alexey Bondar, Josef Lazar

Coumarin as a general switching auxiliary to prepare photochromic and spontaneously blinking fluorophores
Fadi M. Jradi, Brian P. English, Timothy A. Brown, Jesse Aaron, Satya Khuon, James A. Galbraith, Catherine G. Galbraith, Luke D. Lavis

Exploring transient states of PAmKate to enable improved cryogenic single-molecule imaging
Davis Perez, Dara P. Dowlatshahi, Christopher A. Azaldegui, Peter D. Dahlberg, W. E. Moerner

m, Brian P. English, Qinsi Zheng, Sandeep Dave, Fariha Rahman, Liangqi Xie, Peng Dong, Ariana N. Tkachuk, Timothy A. Brown, Robert H. Singer, Zhe Liu, Catherine G. Galbraith, Siegfried M. Musser, Wesley R. Legant, Luke D. Lavis