Microscopy preprints – new tools and techniques in imaging

Here is a curated selection of preprints posted recently on new tools and techniques in imaging. Let us know if we are missing any preprints that are on your reading list!

CaBLAM! A high-contrast bioluminescent Ca2+ indicator derived from an engineered Oplophorus gracilirostris luciferase
Gerard G. Lambert, Emmanuel L. Crespo, Jeremy Murphy, Daniela Boassa, Selena Luong, Junru Hu, Brittany Sprecher, Maya O. Tree, Richard Orcutt, Daniel Heydari, Aidan B. Bell, Albertina Torreblanca Zanca, Ali Hakimi, Diane Lipscombe, Christopher I. Moore, Ute Hochgeschwender, Nathan C. Shaner

Three-dimensional single-cell transcriptome imaging of thick tissues
Rongxin Fang, Aaron R. Halpern, Mohammed Mostafizur Rahman, Zhengkai Huang, Zhiyun Lei, Sebastian J. Hell, Catherine Dulac, Xiaowei Zhuang

Fluorogenesis: Inducing Fluorescence in a Non-Fluorescent Protein Through Photoinduced Chromophore Transfer of a Genetically Encoded Chromophore
Yashwant Kumar, Reman Kumar Singh, Manisha Ojha, Karthik Pushpavanam

A modular chemigenetic calcium indicator enables in vivo functional imaging with near-infrared light
Helen Farrants, Yichun Shuai, William C. Lemon, Christian Monroy Hernandez, Shang Yang, Ronak Patel, Guanda Qiao, Michelle S. Frei, Jonathan B. Grimm, Timothy L. Hanson, Filip Tomaska, Glenn C. Turner, Carsen Stringer, Philipp J. Keller, Abraham G. Beyene, Yao Chen, Yajie Liang, Luke D. Lavis, Eric R. Schreiter

Magnetic control of GFP-like fluorescent proteins
Rebecca Frank Hayward, Julia R. Lazzari-Dean, Andrew G. York* and Maria Ingaramo†

Developing a Toolbox of Antibodies Validated for Array Tomography-Based Imaging of Brain Synapses
Kristina D. Micheva, Belvin Gong, Forrest Collman, Richard J. Weinberg, Stephen J. Smith, James S. Trimmer, Karl D. Murray

Imaging proteins sensitive to direct fusions using transient peptide-peptide interactions
Zoe Gidden, Curran Oi, Emily Johnston, Haresh Bhaskar, Susan Rosser, Simon G. J. Mochrie, Mathew H. Horrocks, Lynne Regan

Technical insights into fluorescence lifetime microscopy of mechanosensitive Flipper probes
Chloé Roffay, Juan Manuel García-Arcos, Pierrik Chapuis, Javier López-Andarias, Falk Schneider, Adai Colom, Caterina Tomba, Ilaria Di Meglio, Valentin Dunsing, Stefan Matile, Aurélien Roux, Vincent Mercier

Multiplexed and millimeter-scale fluorescence nanoscopy of cells and tissue sections via prism-illumination and microfluidics-enhanced DNA-PAINT
Matthew J Rames, John Kenison, Daniel Heineck, Fehmi Civitci, Malwina Szczepaniak, Ting Zheng, Julia Shangguan, Yujia Zhang, Sadik Esener, Xiaolin Nan

Integrated Fluorescence Microscopy (iFLM) for Cryo-FIB-milling and In-situ Cryo-ET
Jae Yang, Veronika Vrbovská, Tilman Franke, Bryan Sibert, Matthew Larson, Alex Hall, Alex Rigort, John Mitchels, Elizabeth R. Wright

Direct optical measurement of intra-molecular distances down to the Ångström scale
Steffen J. Sahl, Jessica Matthias, Kaushik Inamdar, Taukeer A. Khan, Michael Weber, Stefan Becker, Christian Griesinger, Johannes Broichhagen, Stefan W. Hell

Quantum-Enhanced Diamond Molecular Tension Microscopy for Quantifying Cellular Forces
Feng Xu, Shuxiang Zhang, Linjie Ma, Yong Hou, Jie Li, Andrej Denisenko, Zifu Li, Joachim Spatz, Jörg Wrachtrup, Qiang Wei, Zhiqin Chu

The Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for large cleared samples
Nikita Vladimirov, Fabian F. Voigt, Thomas Naert, Gabriela R. Araujo, Ruiyao Cai, Anna Maria Reuss, Shan Zhao, Patricia Schmid, Sven Hildebrand, Martina Schaettin, Dominik Groos, José María Mateos, Philipp Bethge, Taiyo Yamamoto, Valentino Aerne, Alard Roebroeck, Ali Ertürk, Adriano Aguzzi, Urs Ziegler, Esther Stoeckli, Laura Baudis, Soeren S. Lienkamp, Fritjof Helmchen

A Versatile and Open Source One- and Two-Photon Light-Sheet Microscope Design
Thomas Panier, Geoffrey Migault, Antoine Hubert, Hugo Trentesaux, Benoît Beaudou, Georges Debrégeas, Volker Bormuth

Pixelation with concentration-encoded effective photons for molecular optical sectioning microscopy
Geng Wang, Rishyashring R. Iyer, Janet E. Sorrells, Edita Aksamitiene, Eric J. Chaney, Carlos A. Renteria, Jaena Park, Jindou Shi, Yi Sun, Stephen A. Boppart, Haohua Tu

Simultaneous Spectral Differentiation of Multiple Fluorophores in Super-resolution Imaging Using a Glass Phase Plate
Sanduni I. Fernando, Jason T. Martineau, Robert J. Hobson, Thien N. Vu, Brian Baker, Brian D. Mueller, Rajesh Menon, Erik M. Jorgensen, Jordan M. Gerton

ATUM-Tomo: A multi-scale approach to cellular ultrastructure by combined volume scanning electron microscopy and electron tomography
Georg Kislinger, Gunar Fabig, Antonia Wehn, Lucia Rodriguez, Hanyi Jiang, Cornelia Niemann, Andrey S. Klymchenko, Nikolaus Plesnila, Thomas Misgeld, Thomas Müller-Reichert, Igor Khalin, Martina Schifferer

Deep intravital brain tumor imaging enabled by tailored three-photon microscopy and analysis
Marc Cicero Schubert, Stella Judith Soyka, Amr Tamimi, Emanuel Maus, Robert Denninger, Niklas Wissmann, Ekin Reyhan, Svenja Kristin Tetzlaff, Carlo Beretta, Michael Drumm, Julian Schroers, Alicia Steffens, Jordain Walshon, Kathleen McCortney, Sabine Heiland, Anna Golebiewska, Felix Tobias Kurz, Wolfgang Wick, Frank Winkler, Anna Kreshuk, Thomas Kuner, Craig Horbinski, Robert Prevedel, Varun Venkataramani

UVA hyperspectral light-sheet microscopy for volumetric metabolic imaging: application to pre-implantation embryo development
J. Morizet, D. Chow, P. Wijesinghe, E. Schartner, G. Dwapanyin, N. Dubost, G. D. Bruce, E. Anckaert, K. Dunning, K. Dholakia

Expansion-assisted selective plane illumination microscopy for nanoscale imaging of centimeter-scale tissues
Adam Glaser, Jayaram Chandrashekar, Joshua Vasquez, Cameron Arshadi, Naveen Ouellette, Xiaoyun Jiang, Judith Baka, Gabor Kovacs, Micah Woodard, Sharmishtaa Seshamani, Kevin Cao, Nathan Clack, Andrew Recknagel, Anna Grim, Pooja Balaram, Emily Turschak, Alan Liddell, John Rohde, Ayana Hellevik, Kevin Takasaki, Lindsey Erion Barner, Molly Logsdon, Chris Chronopoulos, Saskia de Vries, Jonathan Ting, Steve Perlmutter, Brian Kalmbach, Nikolai Dembrow, R. Clay Reid, David Feng, Karel Svoboda

Real-time targeted illumination in widefield microscopy achieves confocal quality neuronal images
Yao L. Wang, Jia Fan, Samuel H. Chung

Pixel-wise programmability enables dynamic high-SNR cameras for high-speed microscopy
Jie Zhang, Jonathan Newman, Zeguan Wang, Yong Qian, Wei Guo, Zhe Sage Chen, Changyang Linghu, Ralph Etienne-Cummings, Eric Fossum, Edward Boyden, Matthew Wilson

Scalable projected Light Sheet Microscopy for high-resolution imaging of living and cleared samples
Yannan Chen, Shradha Chauhan, Cheng Gong, Hannah Dayton, Cong Xu, Estanislao Daniel De La Cruz, Malika S. Datta, Kam W. Leong, Lars E.P. Dietrich, Raju Tomer

Large-scale deep tissue voltage imaging with targeted illumination confocal microscopy
Sheng Xiao, William J. Cunningham, Krishnakanth Kondabolu, Eric Lowet, Maria V. Moya, Rebecca Mount, Cara Ravasio, Michael N. Economo, Xue Han, Jerome Mertz

Selective plane activation structured illumination microscopy
Kenta Temma, Ryosuke Oketani, Toshiki Kubo, Kazuki Bando, Shunsuke Maeda, Kazunori Sugiura, Tomoki Matsuda, Rainer Heintzmann, Tatsuya Kaminishi, Koki Fukuda, Maho Hamasaki, Takeharu Nagai, Katsumasa Fujita

Correlative superresolution microscopy with deep UV reactivation
Kirti Prakash

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