Vote for the cover – #JCSImagingSI

1. Myo5b KO organoids – Amy Engevik

Intestinal organoids represent an in vitro system that closely recapitulates in vivo cell biology and physiology. These intestinal organoids were generated from mice lacking Myosin 5b which results in cellular abnormalities including apical membrane proteins being retained subapically. This micrograph was acquired on a Zeiss Axiocam microscope.

2. Neuronal Space Invaders – Ciarán Butler-Hallissey

These rat hippocampal neurons are labelled to show microtubules in blue and MAP2 in orange. The image was acquired on spinning disk confocal microscope.

3. Neuronal galaxy – Mari Angeles Juanes

Mature neurons were fixed and stained with MAP2 antibody (neurite marker, cyan), Alexa Fluor 488-phalloidin to visualize F-actin, and DAPI (nuclei, yellow). The image shows the connections between the actin and microtubule cytoskeletons. The images was acquired at 20x on a widefield Leica DMi8 microscope, merged and colour-coded in Fiji.

4. Breaking Symmetry – Antara Chakraborty

WT-MEF labelled with phalloidin (actin – green), acetylated tubulin antibody (magenta) and DAPI (DNA – blue). The image was captured using Laser scanning confocal microscope 780. Deconvolution was done on Huygens Professional version 16.10 and the image was reconstructed using ImageJ.

5. Cell Cytoskeleton – Joe McKellar

This image depicts an African Green Monkey cell stained for actin (white), microtubules (gold) and DNA (orange). The image was taken with an LSM 980 confocal microscope with Airyscan 2 module in Airyscan mode using a 63x lens. Airyscan processing was done on ZEN Blue and post-processing was done on Fiji.

6. Rising tide – Christophe Leterrier

COS-7 cell labeled for actin (phalloidin, gray), ER (RTN4, yellow) and clathrin (cyan) imaged by 3D-SIM, processed with Fiji.

7. Dynamic atrial architecture – Aaron Scott

The myocardial actin networks of the adult zebrafish atrial chamber, bulging with blood cells. The heart was fixed and stained to show nuclei (hot purple) and F-actin networks (grey), and then mounted in agarose before imaging. The image was acquired using Leica SP8 AOBS confocal laser scanning microscope and reconstructed in ImageJ.

8. Mouse Podocyte – Takayuki Miyaki

3D ultrastructural reconstruction of a single mouse podocyte. Podocytes are specialized epithelial cells for blood filtration in the kidney glomeruli. This reconstruction, performed using Amira, is based on complete serial sectional images of a whole glomerulus obtained using field-emission electron microscopy (FE-SEM). The podocyte’s luminal cell membrane (purple) is partially translucent, indicating the localization of the organelle (yellow, centrosome; red, lysosomes; green, mitochondria; dark blue, nucleus; light blue, Golgi apparatuses). The yellow membrane is the basal cell membrane.

9. Intricate Interactions: The Dance of Microtubules and Actin – Wen Lu

The image displays the intricate internal cytoskeletal structure of a nurse cell within a Drosophila ovary. Microtubules were labelled with EMTB-3XGFP (yellow), and actin filaments were stained with Rhodamine-conjugated phalloidin (magenta). The image is a maximum intensity projection of a Z-stack image, acquired on DeepSIM by CrestOptics, and it was processed in Fiji.

10. Pebbles – Ioakeim (Makis) Ampartzidis

In this image, the actin cytoskeleton of neuroepithelial cells is shown in green, and the membranes of some randomly selected cells are highlighted in yellow using synthetic fluorescent labelling dyes. The apical membranes of some cells were pseudo-coloured based on their size, ranging from light blue (small size) to orange (large apical area). The image was taken with the ZEISS LSM880 upright microscope with Airyscan imaging and processed in ImageJ.