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FocalPlane features... Pavel Tomancak

Posted by , on 7 June 2021

In case you missed ‘FocalPlane features…’ this month, here is the recording of the webinar given by Pavel Tomancak on Tuesday 1 June 2021. Light sheet microscopy has been over more than a decade producing stunning new views of the dynamics of living systems. I have been observing the rapid growth of this technology, contributed
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FocalPlane features... Jennifer Lippincott-Schwartz

Posted by , on 1 June 2021

In case you missed our first ‘FocalPlane features…’ event, here is the recording of the webinar given by Jennifer Lippincott-Schwartz on Tuesday 4 May 2021. Powerful new ways to image the internal structures and complex dynamics of cells are revolutionizing cell biology and bio-medical research. In this talk, I will focus on how emerging fluorescent
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Your microscopy news digest is here!

Posted by , on 28 May 2021

Twitter, microscopy forums, and bioimage analysis platforms such as image.sc, … It is difficult to keep up to date with all the discussions and news happening on the different online platforms. To address this, we have created a ‘news digest’ post to help you stay informed about what’s happening in the microscopy community. Here is
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What is Expansion Microscopy?

Sponsored by Andor, on 27 May 2021

How can you get the most information from expanded samples? Traditional light microscopy is limited by the diffraction of light, consequently, features less than 200 nm apart cannot be resolved. For a significant time microscopy technique development was focused towards improving imaging techniques to allow for individual molecules to be resolved. Super-resolution microscopy was developed
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Preparing your manuscript: guidelines for writing microscopy methods and figures

Posted by , on 25 May 2021

A new paper has caught your eye on Twitter or Pubmed based on the title. Next step? A quick look at the abstract. Still interesting? Let’s have a look at the figures. This is probably the most important element of the paper to attract the interest of the reader. If figures are easy to interpret
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Phototoxicity - the good, the bad and the quantified.

Posted by , on 14 May 2021

Our virtual meeting on phototoxicity was held in late January 2021, generously sponsored by the European Microscopy Society and enabled by the Royal Microscopical Society. In four hours, spread over two days, the five organisers and twenty invited participants discussed the problem of phototoxicity in live imaging, and how we can start to tackle this
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Compact and High Performance Fluorescence Microscopy

Sponsored by Etaluma, on 28 April 2021

“Disruptive technologies typically enable new markets to emerge… disruptive products are simpler and cheaper;” Clayton M. Christensen, The Innovator’s Dilemma Microscopes are the most common laboratory instrument in the world; there are more labs with a microscope in them than any other instrument.  As such, it is a field where innovation is constantly producing new
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Part V The future: The hope of smart microscopes and phantoms

Posted by , on 24 April 2021

Elisabeth Kugler 1 and Emmanuel G. Reynaud 2 Contact: kugler.elisabeth@gmail.com; emmanuel.reynaud@ucd.ie We are reaching the end of our LSFM journey. Now it is time to look into the future, riding the plane of light! So, what is next for the LSFM? 1. Four-to-two, then four, then one. The story of SPIM, and LSFM per se,
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A biologist’s checklist for calcium imaging and optogenetic analysis

Posted by , on 12 April 2021

Technological advancement constantly makes these methods more accessible, however, there are a number of understated complexities involved with these types of imaging-based experiments
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Hosting a Focal Plane Journal Club: testimonials from our speakers

Posted by , on 17 March 2021

On 13 May 2020 we hosted our first Journal Club meeting. The aim of these meetings was to select microscopy-related papers and discuss them in a relaxed atmosphere. Everyone was welcome to participate, and we gave special attention to early-career scientists (from undergraduate students to postdocs) who might not have the opportunity to participate in
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