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Highlighting signalling biosensors 1.

Posted by , on 16 January 2025

Why there is interest in signalling biosensors, considerations for using biosensors, and the original biosensor, GFP. Why write about signalling biosensors? In my first postdoctoral position, I was introduced to the concept of endosomal signalling- a way for receptor signalling outcomes to be changed by delivering a signal to different regions of the cell. I
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Probes 101 – How to choose a fluorescent probe for your imaging experiment

Posted by , on 14 December 2024

Fluorescence microscopy is a powerful tool for the visualization of the intricate biological processes occurring within biological organisms. Just like you can make important pieces of information pop up in their notes using colorful highlighters, biologists have at hand a large toolkit of “highlighters” to make their favorite targets stand out in the complex cellular
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Teaching co-localisation analysis from lecture to leisure

Posted by , on 20 October 2024

The origin There was one thing I was always skeptical about: colocalization analysis. When I started out as a BioImage Analyst, it was one of the first things I had to help users with in the BIOP at EPFL. I started learning more about it (and still continue to do so), tried with my own
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How to write a bug report

Posted by , on 3 April 2024

Imagine you try out a software tool for the analysis of your data and for some reason it is not working in the way you expected. Neither the user documentation, nor forums like image.sc or stack overflow can provide answers. Then it is likely that you either found a bug in the software tool or
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StayGold variants explained!

Posted by , on 19 December 2023

I figured it might be useful as a reference, so here’s a blog-ified version of a thread I posted on X about recently published monomeric variants of the StayGold fluorescent protein. Thanks to Helen Zenner of FocalPlane for the help! In 2022, the Katayama/Miyawaki labs published StayGold, a bright and stable fluorescent protein (FP) from
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A general strategy to develop fluorogenic polymethine dyes for bioimaging

Posted by , on 6 December 2023

by Annabell Martin and Pablo Rivera-Fuentes. Fluorescence imaging is an invaluable tool to study biological processes and further progress depends on the development of novel advanced fluorescent probes. Excitingly, using polymethines as a scaffold for fluorogenic dyes, we have opened up a lot of new avenues for bioimaging.
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Community collective: imaging the secretory pathway

Posted by , on 2 November 2023

Does anyone have a favourite fixation protocol for preserving the ER and Golgi? We unroll the advice from the community!
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Sharing Your Poster on Figshare: A Community Guide to How-To and Why

Posted by , on 26 July 2023

Join the Open Science revolution and increase the impact of your research by sharing it on Figshare. Discover the benefits of transparency, collaboration, and accessibility in science as you make your work citable, shareable, and discoverable. Our step-by-step guide will walk you through uploading your conference posters and data, ensuring your research reaches a wider
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Community collective: essential optimisation

Posted by , on 29 June 2023

Microscopy images have the ability to wow us, and the data we can extract from them can help answer our research questions, as well as leading us to pose new ones. Advances in sample preparation and microscopy technology means we have never been in a better position to use imaging in biology but is it
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Community collective: viewing z-stacks in 3D

Posted by , on 14 June 2023

Have you ever sat at your computer and wondered if you have the best representation of your imaging data? Would another software give you better (or different) results? Joy Franco (@engineering_joy) asked the science community on Twitter for their software recommendations for viewing confocal Z-stacks in 3D. Joy’s top request was being able to finely
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