Fluorescent Proteins

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Microscopy preprints – new tools and techniques in imaging

Posted by FocalPlane, on 13 June 2025

Here is a curated selection of preprints posted recently on new tools and techniques in imaging. Let us know if we are missing any recent preprints that are on your reading list! IntAct-U-ExM: Ultrastructure Expansion microscopy of actin networks via an internally-tagged actinAnubhav Dhar, Nishaant Kumar Palani Balaji, Sanjana Mullick, Sucheta Dey, Angana Ghosh, Deepak

Highlighting signalling biosensors 1.

Posted by Greg Redpath, on 16 January 2025

Why there is interest in signalling biosensors, considerations for using biosensors, and the original biosensor, GFP. Why write about signalling biosensors? In my first postdoctoral position, I was introduced to the concept of endosomal signalling- a way for receptor signalling outcomes to be changed by delivering a signal to different regions of the cell. I

Probes 101 – How to choose a fluorescent probe for your imaging experiment

Posted by Lina El Hajji, on 14 December 2024

Fluorescence microscopy is a powerful tool for the visualization of the intricate biological processes occurring within biological organisms. Just like you can make important pieces of information pop up in their notes using colorful highlighters, biologists have at hand a large toolkit of “highlighters” to make their favorite targets stand out in the complex cellular

StayGold variants explained!

Posted by Christophe Leterrier, on 19 December 2023

I figured it might be useful as a reference, so here’s a blog-ified version of a thread I posted on X about recently published monomeric variants of the StayGold fluorescent protein. Thanks to Helen Zenner of FocalPlane for the help! In 2022, the Katayama/Miyawaki labs published StayGold, a bright and stable fluorescent protein (FP) from

60 years of Fluorescent Proteins

Posted by Joachim Goedhart, on 4 October 2022

In talks about fluorescent proteins I usually include a timeline of events related to Green Fluorescent Protein (GFP). The timeline highlights some of the key moments in the history of fluorescent protein discovery and engineering. I am generally fond of timelines, since they provide a way to pay tribute to the pioneers, and other researchers

Primed conversion: the Basics

Posted by Konstantinos Kalyviotis, on 15 November 2021

It has been a bit more than 6 years since the first “primed conversion” article1 was published in May 2015 and around 3 years since I first heard about this new photoconversion technique. I recall that it was almost 1 year after I got my MSc in Greece when I contacted Dr Periklis (Laki) Pantazis

Considerations for expression of fluorescent proteins and imaging in mammalian cells

Posted by Daniel Cabezas de la Fuente, on 7 July 2021

Introduction to fluorescent proteins Fluorescent proteins have the property of absorbing light at one wavelength and emit light in a longer wavelength. These proteins were observed first in bioluminescent organisms known to humanity for centuries. We can find examples of light-emitting organisms in multiple taxa: from single cell organisms like bacteria, to vertebrates like fish.

My history with GFP (and other members of the family)

Posted by Joachim Goedhart, on 7 July 2020

I first crossed paths with green fluorescent protein (GFP) while doing an internship in 1996 at the Max Planck Institute in Göttingen. I was chemically labeling epidermal growth factor (EGF) with the fluorescent dyes Cy3 and Cy5 to study growth-factor signaling. Two post-docs were discussing the use of GFP as an alternative fluorescent tag and

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Microscopy-related articles from our journals

HCR spectral imaging: 10-plex, quantitative, high-resolution RNA and protein imaging in highly autofluorescent samples Development 2024 151: dev202307
filoVision – using deep learning and tip markers to automate filopodia analysis J Cell Sci 2024 137: jcs261274
C omputational tools for quantifying actin filament numbers, lengths, and bundling Biology Open 2024 13: bio.060267

Microscopy-related preprint highlights

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Microscopy-related articles from our journals

Microscopy-related preprint highlights

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