Microscopy preprints – applications in cell and developmental biology

Posted by FocalPlane, on 17 February 2023

Here is a curated selection of preprints published recently. In this post we have a bumper crop of preprints using microscopy tools in cell biology and developmental biology.

Anatomy of the complete mouse eye vasculature in development and pathology explored by light-sheet fluorescence microscopy
Luc Krimpenfort, Maria Garcia-Collado, Tom van Leeuwen, Filippo Locri, Anna-Liisa Luik, Antonio Queiro-Palou, Shigeaki Kanatani, Helder André, Per Uhlén, Lars Jakobsson

Figure extracted from Krimpenfort, *et al.*

Label-free single-cell live imaging reveals fast metabolic switch in T lymphocytes
Noémie Paillon, Thi Phuong Lien Ung, Stéphanie Dogniaux, Chiara Stringari, Claire Hivroz

Multiscale imaging and quantitative analysis of plasma membrane protein-cortical actin interplay
Aparajita Dasgupta, Huong-Tra Ngo, Deryl Tschoerner, Nicolas Touret, Bruno da Rocha-Azevedo, Khuloud Jaqaman

Figure extracted from Dasgupta, *et al.*

Remote-refocusing light-sheet fluorescence microscopy enables 3D imaging of electromechanical coupling of hiPSC-derived and adult cardiomyocytes in co-culture
L Dvinskikh, H Sparks, L Brito, K MacLeod, SE Harding, C Dunsby

Figure extracted from Dvinskikh, *et al.*

Kinetic modelling of sterol transport between plasma membrane and endo-lysosomes based on quantitative fluorescence and X-ray imaging data
Daniel Wüstner, Alice Dupont Juhl, Jacob Marcus Egebjerg, Stephan Werner, James McNally, Gerd Schneider

Targeted volume Correlative Light and Electron Microscopy of an environmental marine microorganism
Karel Mocaer, Giulia Mizzon, Manuel Gunkel, Aliaksandr Halavatyi, Anna Steyer, Viola Oorschot, Martin Schorb, Charlotte Le Kieffre, Daniel P. Yee, Fabien Chevalier, Benoit Gallet, Johan Decelle, Yannick Schwab, Paolo Ronchi

Specialized actin nanoscale layers control focal adhesion turnover
Reena Kumari, Katharina Ven, Megan Chastney, Johan Peranen, Jesse Arron, Leonardo Almeida-Souza, Elena Kremneva, Renaud Poincloux, Teng Leong Chew, Peter W. Gunning, Johanna Ivaska, Pekka Lappalainen

Combining organotypic tissue culture with multi-color fluorescence light-sheet microscopy (OTCxLSFM) – a novel tool to study glioma invasion/migration
Alicia Haydo, Andrej Wehle, Christel Herold-Mende, Donat Kögel, Francesco Pampaloni, Benedikt Linder

Plasmodium ARK2-EB1 axis drives the unconventional spindle dynamics, scaffold formation and chromosome segregation of sexual transmission stages
Mohammad Zeeshan, Edward Rea, Steven Abel, Kruno Vukušić, Robert Markus, Declan Brady, Antonius Eze, Ravish Raspa, Aurelia Balestra, Andrew R. Bottrill, Mathieu Brochet, David S. Guttery, Iva M. Tolić, Anthony A. Holder, Karine G. Le Roch, Eelco C. Tromer, Rita Tewari

Effect of caffeine and other xanthines on liver sinusoidal endothelial cell ultrastructure
Hong Mao, Karolina Szafranska, Larissa Kruse, Christopher Holte, Deanna L. Wolfson, Balpreet Singh Ahluwalia, Cynthia B. Whitchurch, Louise Cole, Glen P. Lockwood, Robin Diekmann, David Le Couteur, Victoria C. Cogger, Peter A.G. McCourt

ISG15-modification of the Arp2/3 complex restricts pathogen spread
Yifeng Zhang, Brittany Ripley, Wei Ouyang, Miranda Sturtz, Ellen Upton, Emma Luhmann, Madeleine Vessely, Rocio Coloma, Nathan Schwery, Scott M. Anthony, Adam Goeken, Thomas O. Moninger, John T. Harty, Aloysius Klingelhutz, Emma Lundberg, David K. Meyerholz, Balaji Manicassamy, Christopher Stipp, Susana Guerra, Lilliana Radoshevich

Hep3D: A 3D single-cell digital atlas of the liver to study spatio-temporal tissue architecture
Dilan Martínez, Valentina Maldonado, Cristian Pérez, Rodrigo Yañez, Valeria Candia, Yannis Kalaidzidis, Marino Zerial, Hernán Morales-Navarrete, Fabián Segovia-Miranda

Figure extracted from Martínez, *et al.*

ATG9A facilitates the biogenesis of influenza A virus liquid condensates near the ER by dissociating recycling vesicles from microtubules
Sílvia Vale-Costa, Temitope Akghibe Etibor, Daniela Brás, Ana Laura Sousa, Maria João Amorim

Figure extracted from Vale-Costa, *et al.*

Mechanical forces at the kidney filtration barrier govern spatial orientation of podocyte processes on capillaries
David Unnersjö-Jess, Amer Ramdedovic, Linus Butt, Ingo Plagmann, Martin Höhne, Agnes Hackl, Hans Blom, Bernhard Schermer, Thomas Benzing

Figure extracted from Unnersjö-Jess, *et al.*

The junctions connecting the endoplasmic reticulum to the nuclear envelope are constricted and remodelled during the cell cycle
Helena Bragulat-Teixidor, Keisuke Ishihara, Gréta Martina Szücs, Shotaro Otsuka

Figure extracted from Bragulat-Teixidor, *et al.*

VAP-mediated membrane tethering mechanisms implicate ER-PM contact function in pH homeostasis
Kar Ling Hoh, Baicong Mu, Tingyi See, Amanda Yunn Ee Ng, Annabel Qi En Ng, Dan Zhang

Figure extracted from Ling Hoh, *et al.*

Filopodial protrusion driven by density-dependent Ena-TOCA-1 interactions
Thomas C. A. Blake, Helen M. Fox, Vasja Urbančič, Adam Wolowczyk, Edward S. Allgeyer, Julia Mason, Jennifer L. Gallop

Mechanically induced topological transition of spectrin regulates its distribution in the mammalian cortex
Andrea Ghisleni, Mayte Bonilla-Quintana, Michele Crestani, Atsushi Fukuzawa, Padmini Rangamani, Nils Gauthier

Evidence for intraflagellar transport in butterfly spermatocyte cilia
Marco Gottardo, Maria Giovanna Riparbelli, Giuliano Callaini, Timothy L. Megraw

Figure extracted from Gottardo, *et al.*

Evolution of the ribbon-like organization of the Golgi apparatus in animal cells
Giovanna Benvenuto, Serena Leone, Emanuele Astoricchio, Sophia Bormke, Sanja Jasek, Enrico D’Aniello, Maike Kittelmann, Kent McDonald, Volker Hartenstein, Valentina Baena, Hector Escriva, Stephanie Bertrand, Bernd Schierwater, Pawel Burkhardt, Inaki Ruiz-Trillo, Gaspar Jekely, Jack Ullrich-Luter, Carsten Luter, Salvatore D’Aniello, Maria Ina Arnone, Francesco Ferraro

Figure extracted from Benvenuto, *et al.*

Age-dependent structural reorganization of utricular ribbon synapses
Susann Michanski, Timo Henneck, Mohona Mukhopadhyay, Anna M. Steyer, Paola Agüi Gonzalez, Katharina Grewe, Peter Ilgen, Mehmet Gültas, Eugenio F. Fornasiero, Stefan Jakobs, Wiebke Möbius, Christian Vogl, Tina Pangršič, Silvio O. Rizzoli, Carolin Wichmann

Self-organizing actin networks drive sequential endocytic protein recruitment and vesicle release on synthetic lipid bilayers
Emily H. Stoops, Michael A. Ferrin, Danielle M. Jorgens, David G. Drubin

Endogenous Tenocyte Activation Underlies the Regenerative Capacity of Adult Zebrafish Tendon
Stephanie L. Tsai, Steffany Villasenor, Rishita Shah, Jenna L. Galloway

Intrinsic microtubule destabilization of multiciliated choroid plexus epithelial cells during postnatal lifetime
Kim Hoa Ho, Valentina Scarpetta, Chiara Salio, Elisa D’Este, Martin Meschkat, Christian A. Wurm, Matthias Kneussel, Carsten Janke, Maria M. Magiera, Marco Sassoè-Pognetto, Monika S. Brill, Annarita Patrizi

Ectopic activation of the polar body extrusion pathway triggers cell fragmentation in preimplantation embryos
Diane Pelzer, Ludmilla de Plater, Peta Bradbury, Adrien Eichmuller, Anne Bourdais, Guillaume Halet, Jean-Léon Maître