Microscopy preprints – applications in cell and developmental biology

Posted by FocalPlane, on 11 August 2023

Here is a curated selection of preprints published recently. In this post, we share preprints that use microscopy tools in cell biology and developmental biology. The microscopy tools featured in these selected preprints include confocal, AFM, SMLM, FLIM, TEM, CLEM, FRAP, SIM, SEM,OCT, cryo-ET, Brillouin and Raman (#loveacroynyms).

Dynein and dynactin move long-range but are delivered separately to the axon tip
Alexander D Fellows, Michaela Bruntraeger, Thomas Burgold, Andrew R Bassett, Andrew P Carter

The Septin Cytoskeleton is Required for Plasma Membrane Repair
M. Isabella Prislusky, Jonathan GT Lam, Viviana Ruiz Contreras, Marilynn Ng, Madeline Chamberlain, Madalyn Fields, Xiaoli Zhang, Amal O. Amer, Stephanie Seveau

The actin binding protein profilin 1 is critical for mitochondria function
Tracy-Ann Read, Bruno A. Cisterna, Kristen Skruber, Samah Ahmadieh, Halli L. Lindamood, Josefine Vitriol, Yang Shi, Austin E.Y.T. Lefebvre, Joseph B. Black, Mitchell T. Butler, James E. Bear, Alena Cherezova, Daria V. Ilatovskaya, Neil L. Weintraub, Eric A. Vitriol

The membrane-actin linkers ezrin, radixin, and moesin are dispensable for macrophage migration and cortex mechanics
Perrine Verdys, Javier Rey Barroso, Joseph Vermeil, Martin Bergert, Thibaut Sanchez, Arnaud Métais, Thomas Mangeat, Elisabeth Bellard, Claire Bigot, Jean-Philippe Girard, Isabelle Maridonneau-Parini, Christel Vérollet, Frédéric Lagarrigue, Alba Diz-Muñoz, Julien Heuvingh, Matthieu Piel, Olivia Du Roure, Véronique Le Cabec, Sébastien Carréno, Renaud Poincloux

Mitochondrial-Derived Compartments are Multilamellar Domains that Encase Membrane Cargo and Cytosol
Zachary N. Wilson, Matt West, Alyssa M. English, Greg Odorizzi, Adam L. Hughes

Figure extracted from Wilson, *et al.* The image is made available under a CC-BY-NC 4.0 International license.

Replisome loading reduces chromatin motion independent of DNA synthesis
Maruthi K. Pabba, Christian Ritter, Vadim O. Chagin, Janis Meyer, Kerem Celikay, Jeffrey H. Stear, Dinah Loerke, Ksenia Kolobynina, Paulina Prorok, Alice Kristin Schmid, Heinrich Leonhardt, Karl Rohr, M. Cristina Cardoso

Figure extracted from Pabba, *et al.* The image is made available under a CC-BY-NC 4.0 International license.

ICAM-1 nanoclusters regulate hepatic epithelial cell polarity by leukocyte adhesion-independent control of apical actomyosin
Cristina Cacho-Navas, Carmen López-Pujante, Natalia Reglero-Real, Natalia Colás-Algora, Ana Cuervo, José Javier Conesa, Susana Barroso, Gema de Rivas, Sergio Ciordia, Alberto Paradela, Gianluca D’Agostino, Carlo Manzo, Jorge Feito, Germán Andrés, Isabel Correas, Jose María Carazo, Sussan Nourshargh, Meritxell Huch, Jaime Millán

Sorting of secretory proteins at the trans-Golgi network by TGN46
Pablo Lujan, Carla Garcia-Cabau, Yuichi Wakana, Javier Vera Lillo, Carmen Rodilla-Ramírez, Vivek Malhotra, Xavier Salvatella, Maria F. Garcia-Parajo, Felix Campelo

The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1
Bruno Carmona, Carolina Camelo, Manon Mehraz, Michel Lemullois, Mariana Lince Faria, Étienne Coyaud, H. Susana Marinho, João Gonçalves, Sofia Nolasco, Francisco Pinto, Brian Raught, Anne-Marie Tassin, France Koll, Helena Soares

Disruption of Fuz in mouse embryos generates hypoplastic hindbrain development and reduced cranial nerve ganglia
Carlo Donato Caiaffa, Yogeshwari S. Ambekar, Manmohan Singh, Ying Linda Lin, Bogdan Wlodarczyk, Salavat R. Aglyamov, Giuliano Scarcelli, Kirill V. Larin, Richard Finnell

Transcriptional clusters follow a conserved condensation-dispersal sequence during stem cell differentiation
Tim Klingberg, Irina Wachter, Agnieszka Pancholi, Yomna Gohar, Priya Kumar, Marcel Sobucki, Elisa Kämmer, Süheyla Eroğlu-Kayıkçı, Sylvia Erhardt, Carmelo Ferrai, Vasily Zaburdaev, Lennart Hilbert

The nuclei of human adult stem cells can move within the cell and generate cellular protrusions to contact other cells
Carlos Bueno, David García-Bernal, Salvador Martínez, Miguel Blanquer, José M. Moraleda

Figure extracted from Bueno, *et al.* The image is made available under a CC-BY 4.0 International license.

Three-dimensional study of spur morphogenesis in the flower of Staphisagria picta (Ranunculaceae) – from cellular level to organ scale
Pauline Delpeuch, Sophie Nadot, Katia Belcram, Antoine Plumerault, Catherine Damerval, Florian Jabbour

Optogenetic control of lysosome function
Nikolay S. Ilyinsky, Sergey M. Bukhalovich, Diana F. Bagaeva, Semen V. Nesterov, Alexey A. Alekseev, Fedor M. Tsybrov, Andrey O. Bogorodskiy, Olga V. Moiseeva, Anastasiya D. Vlasova, Kirill V. Kovalev, Anatoliy E. Mikhailov, Andrey V. Rogachev, Ernst Bamberg, Valentin Ivanovich, Valentin I. Borshchevskiy

Nanoscale analysis of human G1 and metaphase chromatin in situ
Jon Ken Chen, Tingsheng Liu, Shujun Cai, Weimei Ruan, Cai Tong Ng, Jian Shi, Uttam Surana, Lu Gan

Disrupting actin filaments enhances glucose-stimulated insulin secretion independent of the cortical actin cytoskeleton
Alexander J. Polino, Xue Wen Ng, Rebecca Rooks, David W. Piston

Figure extracted from Polino, *et al.* The image is made available under a CC-BY-NC 4.0 International license.

Super-resolution optical imaging reveals accumulation of small mitochondria in the nucleoli of mouse embryos and stem cells
Kezhou Qin, Xinyi Wu

Rho-associated kinase regulates Langerhans cell morphology and responsiveness to tissue damage
Eric Peterman, Elgene J.A. Quitevis, Camille E.A. Goo, Jeffrey P. Rasmussen

Figure extracted from Peterman, *et al.* The image is made available under a CC-BY-NC 4.0 International license.

Peripheral positioning of lysosomes supports melanoma aggressiveness
K. Jerabkova-Roda, A. Mousson, M. Peralta, R. Karali, H. Justiniano, L.M. Lisii, P. Carl, N. Asokan, I. Busnelli, A. Larnicol, O. Lefebvre, H. Lachuer, A. Pichot, T. Stemmelen, A. Molitor, A. Hirschler, F. Delalande, E. Sick, R. Carapito, C. Carapito, V. Hyenne, K. Schauer, P. Rondé, J.G. Goetz

Spatiotemporal precise optical manipulation of intracellular molecular activities
Bin Dong, Shivam Mahapatra, Matthew G. Clark, Mark Carlsen, Karsten J. Mohn, Seohee Ma, Kent Brasseale, Grace Crim, Chi Zhang

ROM1 is redundant to PRPH2 as a molecular building block of photoreceptor disc rims
Tylor R. Lewis, Mustafa S. Makia, Carson M. Castillo, Ying Hao, Muayyad R. Al-Ubaidi, Nikolai P. Skiba, Shannon M. Conley, Vadim Y. Arshavsky, Muna I. Naash

Figure extracted from Lewis, *et al.* The image is made available under a CC-BY-NC 4.0 International license.

Autophagy preferentially degrades non-fibrillar polyQ aggregates
Dorothy Y. Zhao, Felix JB Bauerlein, Itika Saha, Franz-Ulrich Hartl, Wolfgang Baumeister, Florian Wilfling

Cofilin-Driven Nuclear Deformation Drives Dendritic Cell Migration through the Extracellular Matrix
Harry Warner, Giulia Franciosa, Guus van der Borg, Felix Faas, Claire Koenig, Rinse de Boer, René Classens, Sjors Maassen, Maksim Baranov, Shweta Mahajan, Deepti Dabral, Britt Coenen, Frans Bianchi, Niek van Hilten, Herre Jelger Risselada, Wouter H. Roos, Jesper Olsen, Laia Querol Cano, Geert van den Bogaart

Actin-Driven Nanotopography Enhances Integrin Molecular Clutch in Developing Tissue
Tianchi Chen, Cecilia Huertas Fernández-Espartero, Abigail Illand, Ching-Ting Tsai, Yang Yang, Benjamin Klapholz, Pierre Jouchet, Mélanie Fabre, Olivier Rossier, Bianxiao Cui, Sandrine Lévêque-Fort, Nicholas H. Brown, Grégory Giannone

Hierarchical Morphogenesis of Swallowtail Butterfly Wing Scale Nanostructures
Kwi Shan Seah, Vinodkumar Saranathan

PIKfyve is required for phagosomal Rab7 acquisition and the delivery and fusion of early macropinosomes to phagosomes
James H. Vines, Catherine M. Buckley, Jason S. King

Role of trafficking protein particle complex 2 in medaka development
Francesca Zappa, Daniela Intartaglia, Andrea M. Guarino, Rossella De Cegli, Cathal Wilson, Francesco G. Salierno, Elena Polishchuk, Nicolina Cristina Sorrentino, Ivan Conte, Maria Antonietta De Matteis

iFlpMosaics: A method for the ratiometric induction and high-throughput comparative analysis of mutant and wildtype cells
Irene Garcia-Gonzalez, Stefano Gambera, Alvaro Regano, Susana F. Rocha, Lourdes Garcia-Ortega, Mariya Lytvyn, Maria S. Sanchez-Muñoz, Aroa Garcia-Cabero, Ivana Zagorac, Wen Luo, Macarena Fernández-Chacón, Verónica Casquero-Garcia, Federica Francesca Lunella, Rui Benedito

Raman micro-spectroscopy reveals the spatial distribution of fumarate in cells and tissues
Marlous Kamp, Jakub Surmacki, Marc Segarra Mondejar, Tim Young, Karolina Chrabaszcz, Fadwa Joud, Vincent Zecchini, Alyson Speed, Christian Frezza, Sarah E Bohndiek

Figure extracted from Kamp, *et al.* The image is made available under a CC-BY 4.0 International license.

VAP-mediated membrane tethering mechanisms implicate ER-PM contact function in pH homeostasis
Kar Ling Hoh, Baicong Mu, Tingyi See, Amanda Yunn Ee Ng, Annabel Qi En Ng, Dan Zhang

Aberrant tissue stiffness impairs neural tube development in Mthfd1l mutant mouse embryos
Yogeshwari S. Ambekar, Carlo Donato Caiaffa, Bogdan Wlodarczyk, Manmohan Singh, Alexander W. Schill, John Steele, Salavat R. Aglyamov, Giuliano Scarcelli, Richard H. Finnell, Kirill V. Larin

Extracellular filaments revealed by affinity capture cryo-electron tomography of lymphocytes
Leeya Engel, Magda Zaoralova, Alexander R Dunn, Stefan L Oliver

Continuous endosomes form functional subdomains and orchestrate rapid membrane trafficking in trypanosomes
Fabian Link, Alyssa Borges, Oliver Karo, Marvin Jungblut, Thomas Müller, Elisabeth Meyer-Natus, Timothy Krüger, Stefan Sachs, Nicola G. Jones, Mary Morphew, Markus Sauer, Christian Stigloher, J. Richard McIntosh, Markus Engstler