Microscopy preprints – applications in biology

Posted by FocalPlane, on 22 September 2023

Here is a curated selection of preprints published recently. In this post, we share preprints that use microscopy tools to answer questions in biology.

Photoemission electron microscopy for connectomics
Kevin M. Boergens, Gregg Wildenberg, Ruiyu Li, Lola Lambert, Amin Moradi, Guido Stam, Rudolf Tromp, Sense Jan van der Molen, Sarah B. King, Narayanan Kasthuri

The activation of INF2 by Piezo1/Ca2+ is required for mesenchymal to amoeboid transition in confined environments
Neelakshi Kar, Alexa P. Caruso, Nicos Prokopiou, Jeremy S. Logue

Morphodynamics of human early brain organoid development
Akanksha Jain, Gilles Gut, Fátima Sanchis-Calleja, Ryoko Okamoto, Simon Streib, Zhisong He, Fides Zenk, Malgorzata Santel, Makiko Seimiya, René Holtackers, Sophie Martina Johanna Jansen, J. Gray Camp, Barbara Treutlein

A cryo-ET study of ciliary rootlet organization
Chris van Hoorn, Andrew P. Carter

Figure extracted from van Hoorn and Carter. The image is made available under a CC-BY 4.0 International license.

Application of fluorescence lifetimes to multi-imaging analysis in plant cells
Tsuyoshi Aoyama, Nagisa Sugimoto, Yoshikatsu Sato

Visualizing interactions of VDAC1 in live cells using a tetracysteine tag
Johannes Pilic, Furkan E. Oflaz, Benjamin Gottschalk, Yusuf C. Erdogan, Wolfgang F. Graier, Roland Malli

Figure extracted from Pilic, *et al.* The image is made available under a CC-BY-NC 4.0 International license.

Mechanosensitive recruitment of Vinculin maintains junction integrity and barrier function at epithelial tricellular junctions
Lotte van den Goor, Jolene Iseler, Katherine Koning, Ann L. Miller

Growth factor-induced desialylation for the fast control of endocytosis
Ewan MacDonald, Alison Forrester, Cesar A. Valades-Cruz, Thomas D. Madsen, Joseph H. R. Hetmanski, Estelle Dransart, Yeap Ng, Rashmi Godbole, Ananthan Akhil Shp, Ludovic Leconte, Valérie Chambon, Debarpan Ghosh, Alexis Pinet, Dhiraj Bhatia, Bérangère Lombard, Damarys Loew, Martin R. Larson, Hakon Leffler, Dirk J. Lefeber, Henrik Clausen, Patrick Caswell, Massiullah Shafaq-Zadah, Satyajit Mayor, Roberto Weigert, Christian Wunder, Ludger Johannes

Targeting monocytic Occludin impairs monocyte transmigration and HIV neuroinvasion
Diana Brychka, Nilda Vanesa Ayala-Nunez, Yonis Bare, Amandine Dupas, Emma Partiot, Vincent Mittelheisser, Vincent Lucansky, Jacky G. Goetz, Nael Osmani, Raphael Gaudin

Long-term three-dimensional high-resolution imaging of live unlabeled small intestinal organoids using low-coherence holotomography
Mahn Jae Lee, Jaehyeok Lee, Jeongmin Ha, Geon Kim, Hye-Jin Kim, Sumin Lee, Bon-Kyoung Koo, YongKeun Park

Figure extracted from Lee, *et al.* The image is made available under a CC-BY 4.0 International license.

Cortactin stabilizes actin branches by bridging activated Arp2/3 to its nucleated actin filament
Tianyang Liu, Luyan Cao, Miroslav Mladenov, Antoine Jegou, Michael Way, Carolyn A. Moores

Figure extracted from Liu, *et al.* The image is made available under a CC-BY 4.0 International license.

Extracellular filaments revealed by affinity capture cryo-electron tomography of lymphocytes
Leeya Engel, Magda Zaoralova, Alexander R. Dunn, Stefan Oliver

Cytoskeletal adaptation following long-term dysregulation of actomyosin in neuronal processes
Bruno A. Cisterna, Kristen Skruber, Makenzie L. Jane, Caleb I. Camesi, Ivan D. Nguyen, Peyton V. Warp, Joseph B. Black, Mitchell T. Butler, James E. Bear, Tracy-Ann Read, Eric A. Vitriol

Development of an undergraduate cell biology laboratory to assess pigmentation and cell size in a zebrafish model of uveal melanoma
Andrea M. Henle

Piezo1 mechanosensing regulates integrin-dependent chemotactic migration in human T cells
Chinky Shiu Chen Liu, Tithi Mandal, Parijat Biswas, Md. Asmaul Hoque, Purbita Bandopadhyay, Bishnu Prasad Sinha, Jafar Sarif, Ranit D’Rozario, Deepak Kumar Sinha, Bidisha Sinha, Dipyaman Ganguly

Endogenous BAX and BAK form mosaic rings of variable size and composition on apoptotic mitochondria
Sarah Vanessa Schweighofer, Daniel C. Jans, Jan Keller-Findeisen, Anne Folmeg, Mark Bates, Stefan Jakobs

Figure extracted from Schweighofer, *et al.* The image is made available under a CC-BY-NC 4.0 International license.

High-resolution imaging of presynaptic ER networks in Atlastin mutants
Mónica C. Quiñones-Frías, Dina M. Ocken, Avital Rodal

Toxoplasma gondii actin filaments are tuned for rapid disassembly and turnover
Kelli L. Hvorecny, Thomas E. Sladewski, Enrique M. De La Cruz, Justin M. Kollman, Aoife T. Heaslip

Epithelial cell chirality emerges through the dynamic concentric pattern of actomyosin cytoskeleton
Takaki Yamamoto, Tomoki Ishibashi, Yuko Mimori-Kiyosue, Sylvain Hiver, Naoko Tokushige, Mitsusuke Tarama, Masatoshi Takeichi, Tatsuo Shibata

Heterogeneity of synaptic connectivity in the fly visual system
Jacqueline Cornean, Sebastian Molina-Obando, Burak Gür, Annika Bast, Giordano Ramos-Traslosheros, Jonas Chojetzki, Lena Lörsch, Maria Ioannidou, Rachita Taneja, Christopher Schnaitmann, Marion Silies

Probing Molecular Diversity and Ultrastructure of Brain Cells with Fluorescent Aptamers
Xiaotang Lu, Yuelong Wu, Peter H. Li, Tao Fang, Richard L. Schalek, Yaxin Su, Jeffery D. Carter, Shashi Gupta, Viren Jain, Nebojsa Janjic, Jeff W. Lichtman

Tau fibrils induce nanoscale membrane damage and nucleate cytosolic tau at lysosomes
Kevin Rose, Tyler Jepson, Sankalp Shukla, Alex Maya-Romero, Martin Kampmann, Ke Xu, James H. Hurley

Figure extracted from Rose, *et al.* The image is made available under a CC-BY-NC 4.0 International license.

Topological damping in an ultrafast giant cell
Ray Chang, Manu Prakash

Mitochondrial protein FKBP8 captures PDZD8 to form mitochondria-ER contacts
Koki Nakamura, Saeko Aoyama-Ishiwatari, Takahiro Nagao, Mohammadreza Paaran, Christopher J. Obara, Yui Sakurai-Saito, Jake Johnston, Yudan Du, Shogo Suga, Masafumi Tsuboi, Makoto Nakakido, Kouhei Tsumoto, Yusuke Kishi, Yukiko Gotoh, Chulhwan Kwak, Hyun-Woo Rhee, Jeong Kon Seo, Hidetaka Kosako, Clint Potter, Bridget Carragher, Jennifer Lippincott-Schwartz, Franck Polleux, Yusuke Hirabayashi

Mutation in SLC45A2 leads to loss of melanin in parrot feathers
Shatadru Ghosh Roy, Anna Bakhrat, Moty Abdu, Sandra Afonso, Paulo Pereira, Miguel Carneiro, Uri Abdu

The nuclei of human adult stem cells can move within the cell and generate cellular protrusions to contact other cells
Carlos Bueno, David Garcia-Bernal, Salvador Martinez, Miguel Blanquer, Jose M Moraleda

Membrane remodeling properties of the Parkinson’s disease protein LRRK2
Xinbo Wang, Javier Espadas, Yumei Wu, Shujun Cai, Jinghua Ge, Lin Shao, Aurélien Roux, Pietro De Camilli

Near-infrared imaging of phytochrome-derived autofluorescence in plant nuclei
Akira Yoshinari, Reika Isoda, Noriyoshi Yagi, Yoshikatsu Sato, Jelmer J Lindeboom, David W Ehrhardt, Wolf B Frommer, Masayoshi Nakamura

Naive primary neutrophils play a dual role in the tumor microenvironment
Kehinde Adebayo Babatunde, Rupsa Datta, Jose M Ayuso, Anna Huttenlocher, Melissa C. Skala, David J Beebe, Sheena C Kerr

Extracellular vesicles, syntaxin 2 and SNAP23 collectively play a role in the receptive uterine microenvironment
Sadaf N Kalam, Samson Dowland, Louise Cole, Laura Lindsay, Christopher R Murphy

Cryo-EM structures of Tau filaments from the brains of mice transgenic for human mutant P301S Tau
Manuel Schweighauser, Alexey G. Murzin, Jennifer Macdonald, Isabelle L. Lavenir, R. Anthony Crowther, Sjors H.W. Scheres, Michel Goedert

Figure extracted from Schweighauser, *et al.* The image is made available under a CC-BY 4.0 International license.

Basal spot junctions of epithelial tissues respond to morphogenetic forces and regulate Hippo signaling
Benjamin Kroeger, Samuel A. Manning, Yoshana Fonseka, Viola Oorschot, Simon A. Crawford, Georg Ramm, Kieran F. Harvey

A Novel Imaging Method (FIM-ID) Reveals that Myofibrillogenesis Plays a Major Role in the Mechanically Induced Growth of Skeletal Muscle
Kent W. Jorgenson, Jamie E. Hibbert, Ramy K. A. Sayed, Anthony N. Lange, Joshua S. Godwin, Paulo H. C. Mesquita, Bradley A. Ruple, Mason C. McIntosh, Andreas N. Kavazis, Michael D. Roberts, Troy A. Hornberger

Vimentin supports cell polarization by enhancing centrosome function and microtubule acetylation
Renita Saldanha, Minh Tri Ho Thanh, Nikhila Krishnan, Heidi Hehnly, Alison Patteson

Enhanced functionalities of immune cells separated by microfludic lattice: assessment based on holotomography
Mahn Jae Lee, Byungyeon Kim, Dohyeon Lee, Geon Kim, Yoonjae Jung, Hee Sik Shin, Sungyong Choi, YongKeun Park

Ultrastructural localization of calcium homeostasis modulator 1 in the taste buds of mouse circumvallate papillae
Rio Ikuta, Yuu Kakinohana, Shun Hamada

A Light sheet fluorescence microscopy and machine learning-based approach to investigate drug and biomarker distribution in whole organs and tumors
Niyanta Kumar, Petr Hrobař, Martin Vagenknecht, Jindrich Soukup, Nadia Patterson, Peter Bloomingdale, Tomoko Freshwater, Sophia Bardehle, Roman Peter, Ruban Mangadu, Cinthia V. Pastuskovas, Chiswili Y. Chabu, Mark T. Cancilla

Mapping human natural killer cell development in pediatric tonsil by imaging mass cytometry and high-resolution microscopy
Everardo Hegewisch-Solloa, Janine E. Melsen, Hiranmayi Ravichandran, André F. Rendeiro, Aharon G. Freud, Bethany Mundy-Bosse, Johannes C. Melms, Shira E. Eisman, Benjamin Izar, Eli Grunstein, Thomas J. Connors, Olivier Elemento, Amir Horowitz, Emily M. Mace

Longitudinal Awake Imaging of Deep Mouse Brain Microvasculature with Super-resolution Ultrasound Localization Microscopy
Yike Wang, Matthew R. Lowerison, Qi You, Bing-Ze Lin, Daniel A. Llano, Pengfei Song