Microscopy preprints – applications in biology

Posted by FocalPlane, on 4 April 2025

Here is a curated selection of preprints published recently. In this post, we share preprints that use microscopy tools to answer questions in biology.

Complementary Volume Electron Microscopy-based approaches reveal ultrastructural changes in germline intercellular bridges of D. melanogaster
Irina Kolotuev, Abigayle Williams, Caroline Kizilyaprak, Stephanie Pellegrino, Lindsay Lewellyn

Figure extracted from Kolotuev, *et al.* The image is made available under a CC-BY 4.0 International license.

Single Objective Light Sheet Microscopy allows high-resolution in vivo brain imaging of Drosophila
Francisco J. Tassara, Mariano Barella, Lourdes Simó, M. Mailén Folgueira Serrao, Micaela Rodríguez-Caron, Juan Ignacio Ispizua, Mark H. Ellisman, Horacio O. de la Iglesia, M. Fernanda Ceriani, Julián Gargiulo

Structure of the Thin Filament in Human iPSC-derived Cardiomyocytes and its Response to Heart Disease
Rahel A. Woldeyes, Masataka Nishiga, Alison S. Vander Roest, Leeya Engel, Prerna Giri, Gabrielle C. Montenegro, Alexander R. Dunn, James A. Spudich, Daniel Bernstein, Michael F. Schmid, Joseph C. Wu, Wah Chiu

Cryo-correlative light and electron tomography of dopaminergic axonal varicosities reveals non-synaptic modulation of cortico-striatal synapses
Paul Lapios, Robin Anger, Vincent Paget-Blanc, Esther Marza, Vladan Lučić, Rémi Fronzes, Etienne Herzog, David Perrais

Structures of H2A.Z-associated human chromatin remodelers SRCAP and TIP60 reveal divergent mechanisms of chromatin engagement
Giho Park, Avinash B. Patel, Carl Wu, Robert K. Louder

Primordial germ cells experience increasing physical confinement and DNA damage during migration in the mouse embryo
Katharine Goodwin, Theresa Anne Emrich, Sebastian Arnold, Katie McDole

Figure extracted from Goodwin, *et al.* The image is made available under a CC-BY 4.0 International license.

Quantification of lipid sorting during clathrin-mediated endocytosis
H. Mathilda Lennartz, Susana da Costa Nunes, Kristin Böhlig, Sascha M. Kuhn, Lior Moneta, Wan Yee Yau, Lukáš Opálka, Juan M. Iglesias-Artola, Carl D. Modes, Alf Honigmann, André Nadler

Granular component sub-phases direct ribosome biogenesis in the nucleolus
Priyanka Dogra, Mylene C. Ferrolino, Suparna Khatun, Michele Tolbert, Qi Miao, Shondra M. Pruett-Miller, Aaron Pitre, Swarnendu Tripathi, George E. Campbell, Richa Bajpai, Toler Freyaldenhoven, Eric Gibbs, Cheon-Gil Park, Richard W. Kriwacki

Real-Time Tracking of Tumor Invasion Dynamics in Organotypic Brain Slices at Subcellular Resolution
Jana B. Petr, Meng-Syuan Lin, Dina Hochuli, Andreas Hierlemann, Martin Baumgartner, Mario M. Modena

Linker histone H1 functions as a liquid-like glue to organize chromatin in living human cells
Masa A. Shimazoe, Charles Phillips, Jan Huertas, Satoru Ide, Sachiko Tamura, Stephen Farr, S. S. Ashwin, Masaki Sasai, Rosana Collepardo-Guevara, Kazuhiro Maeshima

Two-pore channel protein 2-mediated calcium release promotes angiopoietin 2 secretion by regulating Rab46-dependent Weibel-Palade body trafficking
Ryan D. Murray, Melissa Rose, Katarina T. Miteva, David J. Beech, Lynn McKeown

Imaging Cell Competition in Ex-Vivo Drosophila Adult Brains
Andrés Gutiérrez-García, Mariana Marques-Reis, Eduardo Moreno

Synaptic Ultrastructural Alterations in Human Focal Cortical Dysplasia: Insights from Volume Electron Microscopy
Gyu Hyun Kim, Na-young Seo, Seung-Ki Kim, Yang Hoon Huh, Ji Yeoun Lee, Kea Joo Lee

Discovery of a novel flagellar filament system underpinning Leishmania adhesion to surfaces
Barrack O Owino, Ryuji Yanase, Alan O. Marron, Flavia Moreira-Leite, Sue Vaughan, Jack D Sunter

Visualizing homology search in living cells
Anoek Friskes, Melanie Snoek, Roel Oldenkamp, Bram van den Broekb.vd., Leila Nahidiazar, Lisa Koob, Amalie E. Dick, Marjolijn Mertz, Rolf Harkes, Benjamin D Rowland, Kees Jalink, Rene H Medema

Population-level morphological analysis of paired CO2- and odor-sensing olfactory neurons in D. melanogaster via volume electron microscopy
Jonathan Choy, Shadi Charara, Kalyani Cauwenberghs, Quintyn McKaughan, Keun-Young Kim, Mark H. Ellisman, Chih-Ying Su

Figure extracted from Choy, *et al.* The image is made available under a CC-BY-NC 4.0 International license.

Distinct localization patterns of actin microfilaments during early cell plate formation in plants through deep learning-based image restoration
Suzuka Kikuchi, Takumi Kotaka, Yuga Hanaki, Minako Ueda, Takumi Higaki

Mechanical fracturing of the extracellular matrix patterns the vertebrate heart
Christopher Chan Jin Jie, Daniel Santos-Oliván, Marie-Christine Ramel, Juliana Sánchez-Posada, Toby G R Andrews, Priscilla Paizakis, Emily S Noël, Alejandro Torres-Sánchez, Rashmi Priya

Figure extracted from Chan Jin Jie, *et al.* The image is made available under a CC-BY 4.0 International license.

Correlative light and electron microscopy reveals the fine circuit structure underlying evidence accumulation in larval zebrafish
Jonathan Boulanger-Weill, Florian Kämpf, Richard L. Schalek, Mariela Petkova, Sumit Kumar Vohra, Jay H. Savaliya, Yuelong Wu, Gregor F. P. Schuhknecht, Heike Naumann, Maren Eberle, Kim N. Kirchberger, Simone Rencken, Isaac H. Bianco, Daniel Baum, Filippo Del Bene, Florian Engert, Jeff W. Lichtman, Armin Bahl

Figure extracted from Boulanger-Weill, *et al.* The image is made available under a CC-BY-NC 4.0 International license.

A high-resolution subcellular map of proteins in ciliated cells
Filippa Bertilsson, Feria Hikmet, Jan N. Hansen, Mathias Uhlén, Loren Méar, Cecilia Lindskog

In-depth 3D Exploration of Autosomal Dominant Polycystic Kidney Disease Through Light Sheet Fluorescence Microscopy
Pablo Delgado-Rodriguez, Itsaso Vitoria, Gonzalo R. Ríos-Muñoz, Lídia Bardia, Nicolás Lamanna-Rama, Laura Nicolas-Saenz, Jon Sporring, María L. Soto-Montenegro, Rafael Aldabe, Julien Colombelli, Arrate Muñoz-Barrutia

Direct cell-to-cell transmission of retrotransposons
Maya Voichek, Andreas Bernhard, Maria Novatchkova, Dominik Handler, Paul Möseneder, Baptiste Rafanel, Peter Duchek, Kirsten-André Senti, Julius Brennecke

Optogenetic stimulation of Lbc GEF-mediated Rho activity dynamics promotes cell invasion
Jessica Wagner, Konstantina Feller, Nicole Schrenke, Nina Schulze, Annette Paschen, Leif Dehmelt, Perihan Nalbant

Figure extracted from Wagner, *et al.* The image is made available under a CC-BY-NC 4.0 International license.

Myelinated fiber labeling and orientation mapping of the human brain with light-sheet fluorescence microscopy
Michele Sorelli, Danila Di Meo, Samuel Bradley, Franco Cheli, Josephine Ramazzotti, Laura Perego, Christophe Destrieux, Patrick R. Hof, Francesco S. Pavone, Giacomo Mazzamuto, Irene Costantini

The Golgi Rim is a Precise Tetraplex of Golgin Proteins that Can Self-Assemble into Filamentous Bands
Maohan Su, Abhijith Radhakrishnan, You Yan, Yuan Tian, Hong Zheng, Ons M’Saad, Morven Graham, Jeff Coleman, Jean N. D. Goder, Xinran Liu, Yongdeng Zhang, Joerg Bewersdorf, James E. Rothman

Figure extracted from Su, *et al.* The image is made available under a CC-BY-NC 4.0 International license.

Super-resolution microscopy of mitochondrial mRNAs
Stefan Stoldt, Frederike Maass, Michael Weber, Sven Dennerlein, Peter Ilgen, Jutta Gärtner, Aysenur Canfes, Sarah V. Schweighofer, Daniel C. Jans, Peter Rehling, Stefan Jakobs

Transcriptional coregulation in cis around a contact insulation site revealed by single-molecule microscopy
Maciej A. Kerlin, Ilham Aboulfath-Ladid, Julia Roensch, Chloé Jaubert, Aude Battistella, Kyra J. E. Borgman, Antoine Coulon

Label-Free Quantification of Apoptosis and Necrosis Using Stimulated Raman Scattering Microscopy
Shivam Mahapatra, Shreya B. Shivpuje, Helen C. Campbell, Boyong Wan, Justin Lomont, B. Dong, Seohee Ma, Karsten J. Mohn, Chi Zhang

Amyloid beta is released by lysosomal exocytosis from hiPSC-derived neurons
Adrianna R Tsang, Manoj Reddy Medapati, Claudia Seah, Jordan M Krupa, Stephen H. Pasternak

Cell-type specific autophagy in root hair forming cells is essential for salt stress tolerance in Arabidopsis thaliana
Jierui Zhao, Christian Löfke, Kai Ching Yeung, Yixuan Chen, Yasin Dagdas

Optical recordings of synaptic vesicle fusion reveal diffusional dispersion
Jurgen Klingauf, Nataliya Glyvuk, Junxiu Duan, Sai Krishnan, Yaroslav Tsytsyura, Ulrike Keller, Lakshmi Penothil Sunil, Georgii Nosov, Jana Hueve, Philipp Selenschik, Carsten Reissner, Markus Missler, Martin Kahms

Lipid packing frustration in the outer leaflet of the plasma membrane prevents scission of caveolae
E. Larsson, A. Kabedev, H. Pace, J. Lindwall, B. Fouzia, R.G. Parton, C.A.S. Bergström, I. Parmryd, M. Bally, R. Lundmark

Microtubule-dependent cell polarity regulates skin-resident macrophage phagocytosis and directed cell migration
Eric Peterman, Andrew Murphy, Ian A. Swinburne, Sean G. Megason, Jeffrey P. Rasmussen

Figure extracted from Peterman, *et al.* The image is made available under a CC-BY-NC 4.0 International license.

A novel membrane contact site in vestibular hair cells
Nicolas Brouilly, Nathalie Pujol, Fabrice Richard, Chantal Cazevieille, Jennifer Stone, Ruth Anne Eatock, Rémy Pujol

Directed insulin secretion from beta cells occurs at cortical sites devoid of microtubules at the edges of ELKS/LL5β patches
Margret Fye, Pranoy Sangowdar, Anissa Jayathilake, Pi’ilani Noguchi, Guoqiang Gu, Irina Kaverina

Kinesin-3 KIF14 Regulates Intraflagellar Transport Dynamics in Primary Cilia
Erika Mikulenková, Petra Pejšková, Roman Podhájecký, Luděk Štěpánek, Martina Huranová, Vladimír Varga, Zdeněk Lanský, Lukáš Čajánek

Measurements of Contractility in Actin Convergence Zone and KIF11-Inhibited Mitotic Spindles
Alexandre Matov, Andrea Bacconi

Alpha-synuclein modulates the positioning of endolysosomes in melanoma cells
Nirjhar M. Aloy, Maria Ericsson, Brandon Hartman, Stephan N. Witt

Flagellar pocket collar biogenesis: Cytoskeletal organization and novel structures in a unicellular parasite
Zelená Marie, Casas Elina, Lambert Chloé, Landrein Nicolas, Dacheux Denis, I. Abesamis Kim, Dong Gang, Varga Vladimir, Derrick. R. Robinson, Bonhivers Mélanie